{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/21306"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/21306","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Development of novel in vitro systems for natural anthocyanin production","abstract":"A reliable in vitro system for natural anthocyanin production in Ajuga pyramidalis 'Metallica Crispa' was developed. We found that 2,4-D was necessary to induce callus culture. WPM salts supplemented with sucrose 30%, IAA 2.26 $\\mu$M, zeatin 3.49 $\\mu$M, and pH 5.7 (adjusted prior autoclaving) was optimal for growth and anthocyanin production in callus cultures. In cell culture suspensions, the medium composition was similar to callus cultures, but sucrose concentration was changed to 50%. Suspension cultures were capable of producing anthocyanins at 41-42 mg $\\cdot$ 100 g FW as compared to 7-8 mg in callus cultures and 10-12 mg in pigmented leaf tissue in vivo. The physical microenvironment including temperature at $25\\sp\\circ$C, and irradiance at a PPF of 150 $\\mu$mol m$\\sp{-2}$ s$\\sp{-1}$ provided by cool-white fluorescent lamps was optimal for the system. Callus cultures of Begonia, Ocimum, and Tradescantia were established for the system. Anthocyanin expression was observed in callus cultures of Begonia and Ocimum in the dark. However, fast growing and friable cultures were not obtained for these two crops. We did obtain friable callus from Tradescantia, but the cultures grew very slowly and never expressed anthocyanin. Anthocyanin from Ajuga suspension cultures was more stable than in vivo leaf extracts. Radiation stability of in vitro anthocyanin from Begonia was not different from in vivo. Stability of anthocyanin from both sources was low compared to Ajuga and Tradescantia. In vivo anthocyanin from Tradescantia was more stable than in vitro. PAL activity was observed in colorless callus cultures of Tradescantia. This result suggested that the lack of anthocyanin expression in the cultures might relate to other enzymes in the anthocyanin biosynthetic pathway.","abstract_html":"A reliable in vitro system for natural anthocyanin production in Ajuga pyramidalis &#x27;Metallica Crispa&#x27; was developed. We found that 2,4-D was necessary to induce callus culture. WPM salts supplemented with sucrose 30%, IAA 2.26 <span class=\"etd-inline-math\">&mu;</span>M, zeatin 3.49 <span class=\"etd-inline-math\">&mu;</span>M, and pH 5.7 (adjusted prior autoclaving) was optimal for growth and anthocyanin production in callus cultures. In cell culture suspensions, the medium composition was similar to callus cultures, but sucrose concentration was changed to 50%. Suspension cultures were capable of producing anthocyanins at 41-42 mg $\\cdot$ 100 g FW as compared to 7-8 mg in callus cultures and 10-12 mg in pigmented leaf tissue in vivo. The physical microenvironment including temperature at $25\\sp\\circ$C, and irradiance at a PPF of 150 <span class=\"etd-inline-math\">&mu;</span>mol m$\\sp{-2}$ s$\\sp{-1}$ provided by cool-white fluorescent lamps was optimal for the system. Callus cultures of Begonia, Ocimum, and Tradescantia were established for the system. Anthocyanin expression was observed in callus cultures of Begonia and Ocimum in the dark. However, fast growing and friable cultures were not obtained for these two crops. We did obtain friable callus from Tradescantia, but the cultures grew very slowly and never expressed anthocyanin. Anthocyanin from Ajuga suspension cultures was more stable than in vivo leaf extracts. Radiation stability of in vitro anthocyanin from Begonia was not different from in vivo. Stability of anthocyanin from both sources was low compared to Ajuga and Tradescantia. In vivo anthocyanin from Tradescantia was more stable than in vitro. PAL activity was observed in colorless callus cultures of Tradescantia. This result suggested that the lack of anthocyanin expression in the cultures might relate to other enzymes in the anthocyanin biosynthetic pathway.","abstract_has_math":true,"creators":["Juthangkoon, Suthin"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Crop Sciences","degree_department":null,"school":null,"contributors":["Smith, Mary Ann Lila"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T13:04:47Z","date_published":"2011-05-07T13:04:47Z","updated_at":"2026-07-22T22:25:17Z","subjects":["Agriculture, Food Science and Technology","Agriculture, Plant Culture","Biology, Plant Physiology"],"languages":["eng"],"rights":["Copyright 1995 Juthangkoon, Suthin"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9624378","(UMI)AAI9624378"],"render_values":[{"text":"AAI9624378","href":null,"code":true},{"text":"(UMI)AAI9624378","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/21306","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Smith, Mary Ann Lila"]},{"key":"dc:creator","label":"Author","values":["Juthangkoon, Suthin"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T13:04:47Z","10000-01-01","1995"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Crop Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Agriculture, Food Science and Technology","Agriculture, Plant Culture","Biology, Plant Physiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1995 Juthangkoon, Suthin"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9624378","(UMI)AAI9624378","http://hdl.handle.net/2142/21306"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["A reliable in vitro system for natural anthocyanin production in Ajuga pyramidalis 'Metallica Crispa' was developed. We found that 2,4-D was necessary to induce callus culture. WPM salts supplemented with sucrose 30%, IAA 2.26 $\\mu$M, zeatin 3.49 $\\mu$M, and pH 5.7 (adjusted prior autoclaving) was optimal for growth and anthocyanin production in callus cultures. In cell culture suspensions, the medium composition was similar to callus cultures, but sucrose concentration was changed to 50%. Suspension cultures were capable of producing anthocyanins at 41-42 mg $\\cdot$ 100 g FW as compared to 7-8 mg in callus cultures and 10-12 mg in pigmented leaf tissue in vivo. The physical microenvironment including temperature at $25\\sp\\circ$C, and irradiance at a PPF of 150 $\\mu$mol m$\\sp{-2}$ s$\\sp{-1}$ provided by cool-white fluorescent lamps was optimal for the system. Callus cultures of Begonia, Ocimum, and Tradescantia were established for the system. Anthocyanin expression was observed in callus cultures of Begonia and Ocimum in the dark. However, fast growing and friable cultures were not obtained for these two crops. We did obtain friable callus from Tradescantia, but the cultures grew very slowly and never expressed anthocyanin. Anthocyanin from Ajuga suspension cultures was more stable than in vivo leaf extracts. Radiation stability of in vitro anthocyanin from Begonia was not different from in vivo. Stability of anthocyanin from both sources was low compared to Ajuga and Tradescantia. In vivo anthocyanin from Tradescantia was more stable than in vitro. PAL activity was observed in colorless callus cultures of Tradescantia. This result suggested that the lack of anthocyanin expression in the cultures might relate to other enzymes in the anthocyanin biosynthetic pathway.","Made available in DSpace on 2011-05-07T13:04:47Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9624378.pdf: 3840013 bytes, checksum: 6822fd0b22b1822072aa28108a9c1963 (MD5) Previous issue date: 1995","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:49:53Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:22:44-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Development of novel in vitro systems for natural anthocyanin production"]}]}],"canonical_facts":{"dc:contributor":["Smith, Mary Ann Lila"],"dc:creator":["Juthangkoon, Suthin"],"dc:date":["2011-05-07T13:04:47Z","10000-01-01","1995"],"dc:description":["A reliable in vitro system for natural anthocyanin production in Ajuga pyramidalis 'Metallica Crispa' was developed. We found that 2,4-D was necessary to induce callus culture. WPM salts supplemented with sucrose 30%, IAA 2.26 $\\mu$M, zeatin 3.49 $\\mu$M, and pH 5.7 (adjusted prior autoclaving) was optimal for growth and anthocyanin production in callus cultures. In cell culture suspensions, the medium composition was similar to callus cultures, but sucrose concentration was changed to 50%. Suspension cultures were capable of producing anthocyanins at 41-42 mg $\\cdot$ 100 g FW as compared to 7-8 mg in callus cultures and 10-12 mg in pigmented leaf tissue in vivo. The physical microenvironment including temperature at $25\\sp\\circ$C, and irradiance at a PPF of 150 $\\mu$mol m$\\sp{-2}$ s$\\sp{-1}$ provided by cool-white fluorescent lamps was optimal for the system. Callus cultures of Begonia, Ocimum, and Tradescantia were established for the system. Anthocyanin expression was observed in callus cultures of Begonia and Ocimum in the dark. However, fast growing and friable cultures were not obtained for these two crops. We did obtain friable callus from Tradescantia, but the cultures grew very slowly and never expressed anthocyanin. Anthocyanin from Ajuga suspension cultures was more stable than in vivo leaf extracts. Radiation stability of in vitro anthocyanin from Begonia was not different from in vivo. Stability of anthocyanin from both sources was low compared to Ajuga and Tradescantia. In vivo anthocyanin from Tradescantia was more stable than in vitro. PAL activity was observed in colorless callus cultures of Tradescantia. This result suggested that the lack of anthocyanin expression in the cultures might relate to other enzymes in the anthocyanin biosynthetic pathway.","Made available in DSpace on 2011-05-07T13:04:47Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9624378.pdf: 3840013 bytes, checksum: 6822fd0b22b1822072aa28108a9c1963 (MD5) Previous issue date: 1995","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:49:53Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:22:44-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9624378","(UMI)AAI9624378","http://hdl.handle.net/2142/21306"],"dc:language":["eng"],"dc:rights":["Copyright 1995 Juthangkoon, Suthin"],"dc:subject":["Agriculture, Food Science and Technology","Agriculture, Plant Culture","Biology, Plant Physiology"],"dc:title":["Development of novel in vitro systems for natural anthocyanin production"],"dc:type":["text"],"thesis:degree_discipline":["Crop Sciences"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:17Z"}