{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/21253"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/21253","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Identification and regulation of genes involved in carbon dioxide fixation in Rhodobacter sphaeroides","abstract":"A region of DNA from the genome of the photoheterotrophic bacterium, Rhodobacter sphaeroides, was shown to contain several genes involved in CO$\\sb2$ fixation, namely fructose 1,6-bisphosphatase (fbpA), phosphoribulokinase (prkA), a 37-kDa polypeptide (designed CFX A) with a putative regulatory role (cfxA), and Form I ribulose 1,5-bisphosphate carboxylase/oxygenase (rbcL,S).","abstract_html":"A region of DNA from the genome of the photoheterotrophic bacterium, Rhodobacter sphaeroides, was shown to contain several genes involved in CO$\\sb2$ fixation, namely fructose 1,6-bisphosphatase (fbpA), phosphoribulokinase (prkA), a 37-kDa polypeptide (designed CFX A) with a putative regulatory role (cfxA), and Form I ribulose 1,5-bisphosphate carboxylase/oxygenase (rbcL,S).","abstract_has_math":true,"creators":["Hallenbeck, Paul Leon"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":["Kaplan, Samuel"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T13:03:05Z","date_published":"2011-05-07T13:03:05Z","updated_at":"2026-07-22T22:25:17Z","subjects":["Biology, Molecular","Biology, Genetics","Biology, Microbiology"],"languages":["eng"],"rights":["Copyright 1989 Hallenbeck, Paul Leon"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9010872","(UMI)AAI9010872"],"render_values":[{"text":"AAI9010872","href":null,"code":true},{"text":"(UMI)AAI9010872","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/21253","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kaplan, Samuel"]},{"key":"dc:creator","label":"Author","values":["Hallenbeck, Paul Leon"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T13:03:05Z","10000-01-01","1989"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Molecular","Biology, Genetics","Biology, Microbiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1989 Hallenbeck, Paul Leon"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9010872","(UMI)AAI9010872","http://hdl.handle.net/2142/21253"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["A region of DNA from the genome of the photoheterotrophic bacterium, Rhodobacter sphaeroides, was shown to contain several genes involved in CO$\\sb2$ fixation, namely fructose 1,6-bisphosphatase (fbpA), phosphoribulokinase (prkA), a 37-kDa polypeptide (designed CFX A) with a putative regulatory role (cfxA), and Form I ribulose 1,5-bisphosphate carboxylase/oxygenase (rbcL,S).","The prkA gene was localized and identified by expression of the prkA gene product in vivo and in vitro. The PRK activity expressed in Escherichia coli was characterized, the product of the reaction identified chemically, and the prkA gene product expressed was identical to the gene product produced in Rb. sphaeroides.","The fbpA and rbcL,S genes were identified and localized by comparing the amino acid sequences deduced from the DNA sequence analysis with the amino acid sequence of known Fbp and RbcL proteins. These genes are all transcribed in the same direction and are tandomly arranged.","A second region of the genome contained a non-identical but similar duplicate set of these genes, namely, fbpB, prkB, cfxB and rbcR but had 3 kb of DNA between the 3$\\sp\\prime$ end of prkB and the 5$\\sp\\prime$ end of cfxB, a portion of which codes for glyceraldehyde 3-phosphate dehydrogenase (gapB).","Strains were constructed which contained null mutations in cfxA and/or cfxB. Each mutation eliminated downstream rbc expression. Studies utilizing these strains demonstrated that each form of ribulose 1,5-bisphosphate carboxylase/oxygenase plays an essential role in maintaining the cellular redox balance during photoheterotrophic growth at differing CO$\\sb2$ concentrations.","Strains were also constructed which contained null mutations in prkA and/or prkB. Studies utilizing these strains demonstrated that CO$\\sb2$ fixation was critical for photoheterotrophic growth but could be replaced by the alternative reduction of dimethylsulfoxide. Thus, CO$\\sb2$ fixation was identified as an essential mediator in maintaining cellular redox balance critical for photoheterotrophic growth. The product of phosphoribulokinase, ribulose 1,5-bisphosphate, is suggested to be one factor involved in the control of expression of ribulose 1,5-bisphosphate carboxylase/oxygenase. Each form of phosphoribulokinase and ribulose 1,5-bisphosphate carboxylase/oxygenase were shown to have distinct roles in CO$\\sb2$ fixation, and the existence and location of a region encoding a repressor of prkA expression and an activator of rbcR expression (cfxR) was postulated.","Made available in DSpace on 2011-05-07T13:03:05Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9010872.pdf: 7886418 bytes, checksum: 2314a7482e22d0ee5fad115319c40bc0 (MD5) Previous issue date: 1989","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:49:31Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:22:32-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Identification and regulation of genes involved in carbon dioxide fixation in Rhodobacter sphaeroides"]}]}],"canonical_facts":{"dc:contributor":["Kaplan, Samuel"],"dc:creator":["Hallenbeck, Paul Leon"],"dc:date":["2011-05-07T13:03:05Z","10000-01-01","1989"],"dc:description":["A region of DNA from the genome of the photoheterotrophic bacterium, Rhodobacter sphaeroides, was shown to contain several genes involved in CO$\\sb2$ fixation, namely fructose 1,6-bisphosphatase (fbpA), phosphoribulokinase (prkA), a 37-kDa polypeptide (designed CFX A) with a putative regulatory role (cfxA), and Form I ribulose 1,5-bisphosphate carboxylase/oxygenase (rbcL,S).","The prkA gene was localized and identified by expression of the prkA gene product in vivo and in vitro. The PRK activity expressed in Escherichia coli was characterized, the product of the reaction identified chemically, and the prkA gene product expressed was identical to the gene product produced in Rb. sphaeroides.","The fbpA and rbcL,S genes were identified and localized by comparing the amino acid sequences deduced from the DNA sequence analysis with the amino acid sequence of known Fbp and RbcL proteins. These genes are all transcribed in the same direction and are tandomly arranged.","A second region of the genome contained a non-identical but similar duplicate set of these genes, namely, fbpB, prkB, cfxB and rbcR but had 3 kb of DNA between the 3$\\sp\\prime$ end of prkB and the 5$\\sp\\prime$ end of cfxB, a portion of which codes for glyceraldehyde 3-phosphate dehydrogenase (gapB).","Strains were constructed which contained null mutations in cfxA and/or cfxB. Each mutation eliminated downstream rbc expression. Studies utilizing these strains demonstrated that each form of ribulose 1,5-bisphosphate carboxylase/oxygenase plays an essential role in maintaining the cellular redox balance during photoheterotrophic growth at differing CO$\\sb2$ concentrations.","Strains were also constructed which contained null mutations in prkA and/or prkB. Studies utilizing these strains demonstrated that CO$\\sb2$ fixation was critical for photoheterotrophic growth but could be replaced by the alternative reduction of dimethylsulfoxide. Thus, CO$\\sb2$ fixation was identified as an essential mediator in maintaining cellular redox balance critical for photoheterotrophic growth. The product of phosphoribulokinase, ribulose 1,5-bisphosphate, is suggested to be one factor involved in the control of expression of ribulose 1,5-bisphosphate carboxylase/oxygenase. Each form of phosphoribulokinase and ribulose 1,5-bisphosphate carboxylase/oxygenase were shown to have distinct roles in CO$\\sb2$ fixation, and the existence and location of a region encoding a repressor of prkA expression and an activator of rbcR expression (cfxR) was postulated.","Made available in DSpace on 2011-05-07T13:03:05Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9010872.pdf: 7886418 bytes, checksum: 2314a7482e22d0ee5fad115319c40bc0 (MD5) Previous issue date: 1989","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:49:31Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:22:32-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9010872","(UMI)AAI9010872","http://hdl.handle.net/2142/21253"],"dc:language":["eng"],"dc:rights":["Copyright 1989 Hallenbeck, Paul Leon"],"dc:subject":["Biology, Molecular","Biology, Genetics","Biology, Microbiology"],"dc:title":["Identification and regulation of genes involved in carbon dioxide fixation in Rhodobacter sphaeroides"],"dc:type":["text"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:17Z"}