{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/21148"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/21148","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Use of RFLP markers in maize as an aid in selection during inbreeding","abstract":"Molecular markers can be used to detect alleles in donor genetic material for improvement of existing cultivars or hybrids. Restriction fragment length polymorphisms (RFLPs) were used as markers to detect favorable quantitative trait alleles in the maize (Zea mays L.) population BS11(FR)C7 which were not in the hybrid 'FRB73 x FRMo17'. Thirty-four RFLP loci representing 16 chromosome arms were used to determine marker genotypes for 220 (BS11(FR)C7 x FRMo17) F2 individuals. Statistical associations between marker loci and trait expression in F2 x FRB73 progeny were found for grain yield, stalk and root lodging, plant and ear height, maturity, and seven grain yield component traits. Many RFLP loci were associated with expression of multiple traits. RFLP alleles from BS11(FR)C7 were favorable over those from FRMo17 for some loci in all but one trait. Digenic epistasis and environmental effects also appeared to influence marker-trait associations.","abstract_html":"Molecular markers can be used to detect alleles in donor genetic material for improvement of existing cultivars or hybrids. Restriction fragment length polymorphisms (RFLPs) were used as markers to detect favorable quantitative trait alleles in the maize (Zea mays L.) population BS11(FR)C7 which were not in the hybrid 'FRB73 x FRMo17'. Thirty-four RFLP loci representing 16 chromosome arms were used to determine marker genotypes for 220 (BS11(FR)C7 x FRMo17) F2 individuals. Statistical associations between marker loci and trait expression in F2 x FRB73 progeny were found for grain yield, stalk and root lodging, plant and ear height, maturity, and seven grain yield component traits. Many RFLP loci were associated with expression of multiple traits. RFLP alleles from BS11(FR)C7 were favorable over those from FRMo17 for some loci in all but one trait. Digenic epistasis and environmental effects also appeared to influence marker-trait associations.","abstract_has_math":false,"creators":["Zehr, Brent Eugene"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Crop Sciences","degree_department":null,"school":null,"contributors":["Dudley, John W."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T12:59:47Z","date_published":"2011-05-07T12:59:47Z","updated_at":"2026-07-22T22:25:17Z","subjects":["Agriculture, Agronomy","Biology, Biostatistics","Biology, Genetics"],"languages":["eng"],"rights":["Copyright 1990 Zehr, Brent Eugene"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9114479","(UMI)AAI9114479"],"render_values":[{"text":"AAI9114479","href":null,"code":true},{"text":"(UMI)AAI9114479","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/21148","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Dudley, John W."]},{"key":"dc:creator","label":"Author","values":["Zehr, Brent Eugene"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T12:59:47Z","10000-01-01","1990"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Crop Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Agriculture, Agronomy","Biology, Biostatistics","Biology, Genetics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1990 Zehr, Brent Eugene"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9114479","(UMI)AAI9114479","http://hdl.handle.net/2142/21148"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Molecular markers can be used to detect alleles in donor genetic material for improvement of existing cultivars or hybrids. Restriction fragment length polymorphisms (RFLPs) were used as markers to detect favorable quantitative trait alleles in the maize (Zea mays L.) population BS11(FR)C7 which were not in the hybrid 'FRB73 x FRMo17'. Thirty-four RFLP loci representing 16 chromosome arms were used to determine marker genotypes for 220 (BS11(FR)C7 x FRMo17) F2 individuals. Statistical associations between marker loci and trait expression in F2 x FRB73 progeny were found for grain yield, stalk and root lodging, plant and ear height, maturity, and seven grain yield component traits. Many RFLP loci were associated with expression of multiple traits. RFLP alleles from BS11(FR)C7 were favorable over those from FRMo17 for some loci in all but one trait. Digenic epistasis and environmental effects also appeared to influence marker-trait associations.","If marker loci are to be useful in selection for quantitative traits, a method of weighting information from different loci is needed. Associations between RFLP loci and grain yield were used to develop indices for selection among F2 individuals, and within heterogeneous 54 families derived from F2 selections. Each index involved a matrix of genotypic weights within RFLP loci and a vector of weights among loci. The optimum RFLP index for selection among F2 individuals consisted of a matrix giving equal weight to homozygous and heterozygous favorable RFLP genotypes, and a vector of relative locus weights according to probability of association with grain yield. Indices using subsets of loci were as effective as indices based on fifteen loci for increasing the average frequency of favorable RFLP alleles over all loci in both F2 and S4 selections. Loci with low index weight had greater influence when RFLP selections were within S4 families. These results indicate that relatively few RFLP markers are needed to screen segregating populations for associated loci affecting expression of complex traits. However, RFLP selection at higher levels of inbreeding would require the use of more markers.","Made available in DSpace on 2011-05-07T12:59:47Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9114479.pdf: 6281366 bytes, checksum: 92cb40883a3a6ed885dd9651ff43799c (MD5) Previous issue date: 1990","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:48:48Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:22:08-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Use of RFLP markers in maize as an aid in selection during inbreeding"]}]}],"canonical_facts":{"dc:contributor":["Dudley, John W."],"dc:creator":["Zehr, Brent Eugene"],"dc:date":["2011-05-07T12:59:47Z","10000-01-01","1990"],"dc:description":["Molecular markers can be used to detect alleles in donor genetic material for improvement of existing cultivars or hybrids. Restriction fragment length polymorphisms (RFLPs) were used as markers to detect favorable quantitative trait alleles in the maize (Zea mays L.) population BS11(FR)C7 which were not in the hybrid 'FRB73 x FRMo17'. Thirty-four RFLP loci representing 16 chromosome arms were used to determine marker genotypes for 220 (BS11(FR)C7 x FRMo17) F2 individuals. Statistical associations between marker loci and trait expression in F2 x FRB73 progeny were found for grain yield, stalk and root lodging, plant and ear height, maturity, and seven grain yield component traits. Many RFLP loci were associated with expression of multiple traits. RFLP alleles from BS11(FR)C7 were favorable over those from FRMo17 for some loci in all but one trait. Digenic epistasis and environmental effects also appeared to influence marker-trait associations.","If marker loci are to be useful in selection for quantitative traits, a method of weighting information from different loci is needed. Associations between RFLP loci and grain yield were used to develop indices for selection among F2 individuals, and within heterogeneous 54 families derived from F2 selections. Each index involved a matrix of genotypic weights within RFLP loci and a vector of weights among loci. The optimum RFLP index for selection among F2 individuals consisted of a matrix giving equal weight to homozygous and heterozygous favorable RFLP genotypes, and a vector of relative locus weights according to probability of association with grain yield. Indices using subsets of loci were as effective as indices based on fifteen loci for increasing the average frequency of favorable RFLP alleles over all loci in both F2 and S4 selections. Loci with low index weight had greater influence when RFLP selections were within S4 families. These results indicate that relatively few RFLP markers are needed to screen segregating populations for associated loci affecting expression of complex traits. However, RFLP selection at higher levels of inbreeding would require the use of more markers.","Made available in DSpace on 2011-05-07T12:59:47Z (GMT). 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