{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/21113"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/21113","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Molecular genetic analysis of puf andpuc operon expression in Rhodobacter sphaeroides 2.4.1","abstract":"The deletion of the puf intercistronic terminator resulted in both the loss of the smallest 0.5-kilobase (kb) puf transcript in addition to an approximate 10-fold increase in transcriptional read-through of the mutated region to more distal pufL gene, suggesting that the proximal intercistronic stem-loop functions as a transcription terminator.","abstract_html":"The deletion of the puf intercistronic terminator resulted in both the loss of the smallest 0.5-kilobase (kb) puf transcript in addition to an approximate 10-fold increase in transcriptional read-through of the mutated region to more distal pufL gene, suggesting that the proximal intercistronic stem-loop functions as a transcription terminator.","abstract_has_math":false,"creators":["Lee, Jeong Kug"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":["Gardner, Jeffrey F."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T12:58:46Z","date_published":"2011-05-07T12:58:46Z","updated_at":"2026-07-22T22:25:17Z","subjects":["Biology, Molecular","Biology, Microbiology"],"languages":["eng"],"rights":["Copyright 1992 Lee, Jeong Kug"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9215846","(UMI)AAI9215846"],"render_values":[{"text":"AAI9215846","href":null,"code":true},{"text":"(UMI)AAI9215846","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/21113","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Gardner, Jeffrey F."]},{"key":"dc:creator","label":"Author","values":["Lee, Jeong Kug"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T12:58:46Z","10000-01-01","1992"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Molecular","Biology, Microbiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1992 Lee, Jeong Kug"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9215846","(UMI)AAI9215846","http://hdl.handle.net/2142/21113"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The deletion of the puf intercistronic terminator resulted in both the loss of the smallest 0.5-kilobase (kb) puf transcript in addition to an approximate 10-fold increase in transcriptional read-through of the mutated region to more distal pufL gene, suggesting that the proximal intercistronic stem-loop functions as a transcription terminator.","The puc operon encodes 2.3- and 0.5-kb transcripts. The 2.3-kb transcript extends from the same 5$\\sp\\prime$ end (117 nucleotides from the start of pucB) as that of the 0.5-kb transcript to approximately 1.8 kb downstream of the pucBA genes, and encodes gene product(s) involved in post-translational expression of the pucBA gene products involved in the formation of B800-850 complex.","A 629-base pair DNA region upstream of the 5$\\sp\\prime$-ends of the two puc-specific transcripts encompasses two functionally separable cis-acting domains: the upstream regulatory region (URS, $-$629 to $-$150) responsible for oxygen and light control of puc operon transcription, and the more proximal downstream regulatory region (DRS, $-$150 to $-$1) containing putative IHF/FNR-binding sites ($-$129 $-$105), putative promoter(s) ($-$92 and $-$57), and operator(s) ($-$52 $-$35 and $-$27 $-$10) involved in oxygen control of puc operon transcription. In addition, data reveal a direct interaction between the URS ($-$629 $-$150) and the DRS ($-$150 $-$1). A trans-acting factor involved in O$\\sb2$ control of puc operon transcription acted upon the puc URS, while a second trans-acting factor(s) acted upon both the puc URS and DRS. The former trans-acting factor was genetically mapped to a 2.2-kb DNA fragment located within the carotenoid gene cluster, while the genetic map of the second trans-acting factor was localized to a 7.0-kb DNA fragment containing the puhA gene and its flanking DNA (6.3 kb).","Made available in DSpace on 2011-05-07T12:58:46Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9215846.pdf: 8265007 bytes, checksum: b6ae4601ec7d5e2cefeb1614596dacef (MD5) Previous issue date: 1992","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:48:34Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:22:00-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Molecular genetic analysis of puf andpuc operon expression in Rhodobacter sphaeroides 2.4.1"]}]}],"canonical_facts":{"dc:contributor":["Gardner, Jeffrey F."],"dc:creator":["Lee, Jeong Kug"],"dc:date":["2011-05-07T12:58:46Z","10000-01-01","1992"],"dc:description":["The deletion of the puf intercistronic terminator resulted in both the loss of the smallest 0.5-kilobase (kb) puf transcript in addition to an approximate 10-fold increase in transcriptional read-through of the mutated region to more distal pufL gene, suggesting that the proximal intercistronic stem-loop functions as a transcription terminator.","The puc operon encodes 2.3- and 0.5-kb transcripts. The 2.3-kb transcript extends from the same 5$\\sp\\prime$ end (117 nucleotides from the start of pucB) as that of the 0.5-kb transcript to approximately 1.8 kb downstream of the pucBA genes, and encodes gene product(s) involved in post-translational expression of the pucBA gene products involved in the formation of B800-850 complex.","A 629-base pair DNA region upstream of the 5$\\sp\\prime$-ends of the two puc-specific transcripts encompasses two functionally separable cis-acting domains: the upstream regulatory region (URS, $-$629 to $-$150) responsible for oxygen and light control of puc operon transcription, and the more proximal downstream regulatory region (DRS, $-$150 to $-$1) containing putative IHF/FNR-binding sites ($-$129 $-$105), putative promoter(s) ($-$92 and $-$57), and operator(s) ($-$52 $-$35 and $-$27 $-$10) involved in oxygen control of puc operon transcription. In addition, data reveal a direct interaction between the URS ($-$629 $-$150) and the DRS ($-$150 $-$1). A trans-acting factor involved in O$\\sb2$ control of puc operon transcription acted upon the puc URS, while a second trans-acting factor(s) acted upon both the puc URS and DRS. The former trans-acting factor was genetically mapped to a 2.2-kb DNA fragment located within the carotenoid gene cluster, while the genetic map of the second trans-acting factor was localized to a 7.0-kb DNA fragment containing the puhA gene and its flanking DNA (6.3 kb).","Made available in DSpace on 2011-05-07T12:58:46Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9215846.pdf: 8265007 bytes, checksum: b6ae4601ec7d5e2cefeb1614596dacef (MD5) Previous issue date: 1992","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:48:34Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:22:00-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9215846","(UMI)AAI9215846","http://hdl.handle.net/2142/21113"],"dc:language":["eng"],"dc:rights":["Copyright 1992 Lee, Jeong Kug"],"dc:subject":["Biology, Molecular","Biology, Microbiology"],"dc:title":["Molecular genetic analysis of puf andpuc operon expression in Rhodobacter sphaeroides 2.4.1"],"dc:type":["text"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:17Z"}