Biochemical and genetic analysis of nucleic acid-protein interactions
type: CreativeWork
description: The integration host factor (IHF) of Escherichia coli is a small, sequence-specific DNA-binding protein. The specific and nonspecific DNA binding constants were determined by gel mobility shift assays. The binding constant of IHF for the H$\sp\prime$ site in $\lambda$ attP is 6.8 $\times$ 10$\sp8$ M$\sp{-1}$ and the nonspecific binding constant is 5.8 $\times$ 10$\sp5$ M$\sp{-1}.$ Therefore, the selectivity of IHF binding is about a thousand-fold higher for a specific site over random sequences. To study the molecular determinants specifying IHF binding, a series of 41 oligonucleotides were synthesized containing adenine analogues that modified the surfaces of the major and minor grooves of DNA. The binding constants determined for these analogue-containing H$\sp\prime$ sites revealed that the specific interaction of IHF with its H$\sp\prime$ site involves interactions with both minor and major grooves of the DNA.
abstract: The integration host factor (IHF) of Escherichia coli is a small, sequence-specific DNA-binding protein. The specific and nonspecific DNA binding constants were determined by gel mobility shift assays. The binding constant of IHF for the H$\sp\prime$ site in $\lambda$ attP is 6.8 $\times$ 10$\sp8$ M$\sp{-1}$ and the nonspecific binding constant is 5.8 $\times$ 10$\sp5$ M$\sp{-1}.$ Therefore, the selectivity of IHF binding is about a thousand-fold higher for a specific site over random sequences. To study the molecular determinants specifying IHF binding, a series of 41 oligonucleotides were synthesized containing adenine analogues that modified the surfaces of the major and minor grooves of DNA. The binding constants determined for these analogue-containing H$\sp\prime$ sites revealed that the specific interaction of IHF with its H$\sp\prime$ site involves interactions with both minor and major grooves of the DNA.
author: Wang, Siqun
language: eng
keywords: Biology, Molecular, Biology, Genetics, Biology, Microbiology
date published: 2011-05-07T12:58:08Z
date modified: 2026-07-22T22:25:17Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/21092