{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/20916"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/20916","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Calcium-dependent changes of apical membrane sodium channel density and open probability in frog skin","abstract":"Blocker-induced noise analysis of Na$\\sp{+}$ channels at apical membranes of intact and isolated epithelia of frog skin was carried out to investigate whether treatment of tissues with ionomycin, quinine, or acidification of basolateral solution shared similar mechanisms of inhibition of Na$\\sp{+}$ absorption. Experiments were done using staircase protocols (SC) that allowed evaluation of the dependence on blocker concentration of the macroscopic rates of Na$\\sp{+}$ transport I$\\sbsp{\\rm Na}{\\rm B}$, single channel Na$\\sp{+}$ current (i$\\sbsp{\\rm Na}{\\rm B}$), on and off blocker rate coefficients as well as determination of channel densities in open and blocked states and the Na$\\sp{+}$ channel open probability in the absence of blocker ($\\beta\\sp\\prime$). In addition to SC experiments, time course (TC) or paired time course (PTC) experiments allowed an assessment of the rates of change of the blocker rate coefficients and the changes of I$\\sbsp{\\rm Na}{\\rm B}$, i$\\sbsp{\\rm Na}{\\rm B}$, and the channel densities. Ionomycin, quinine, and acidification of the basolateral solution, despite their differing methods of increase of cytosolic Ca$\\sp{++}$ activity, caused in common inhibition of total channel density (N$\\sb{\\rm T}$) and stimulation of open channel density (N$\\sb{\\rm o}$) by way of increase of open channel probability ($\\beta\\sp\\prime$). Time course experiments revealed that inhibition of N$\\sb{\\rm T}$ and stimulation of $\\beta\\sp\\prime$ were delayed by several minutes, with relatively large changes of N$\\sb{\\rm T}$ and $\\beta\\sp\\prime$ occurring over 30 to 60 minutes. These observations support the view that Ca$\\sp{++}$-related mechanisms of regulation of N$\\sb{\\rm T}$ and $\\beta\\sp\\prime$ are slow with respect to reported rates of increase of intracellular Ca$\\sp{++}$ and are thus incompatible with a direct effect of Ca$\\sp{++}$ on the epithelial Na$\\sp{+}$ channel.","abstract_html":"Blocker-induced noise analysis of Na$\\sp{+}$ channels at apical membranes of intact and isolated epithelia of frog skin was carried out to investigate whether treatment of tissues with ionomycin, quinine, or acidification of basolateral solution shared similar mechanisms of inhibition of Na$\\sp{+}$ absorption. Experiments were done using staircase protocols (SC) that allowed evaluation of the dependence on blocker concentration of the macroscopic rates of Na$\\sp{+}$ transport I$\\sbsp{\\rm Na}{\\rm B}$, single channel Na$\\sp{+}$ current (i$\\sbsp{\\rm Na}{\\rm B}$), on and off blocker rate coefficients as well as determination of channel densities in open and blocked states and the Na$\\sp{+}$ channel open probability in the absence of blocker (<span class=\"etd-inline-math\">&beta;\\sp\\prime</span>). In addition to SC experiments, time course (TC) or paired time course (PTC) experiments allowed an assessment of the rates of change of the blocker rate coefficients and the changes of I$\\sbsp{\\rm Na}{\\rm B}$, i$\\sbsp{\\rm Na}{\\rm B}$, and the channel densities. Ionomycin, quinine, and acidification of the basolateral solution, despite their differing methods of increase of cytosolic Ca$\\sp{++}$ activity, caused in common inhibition of total channel density (N$\\sb{\\rm T}$) and stimulation of open channel density (N$\\sb{\\rm o}$) by way of increase of open channel probability (<span class=\"etd-inline-math\">&beta;\\sp\\prime</span>). Time course experiments revealed that inhibition of N$\\sb{\\rm T}$ and stimulation of <span class=\"etd-inline-math\">&beta;\\sp\\prime</span> were delayed by several minutes, with relatively large changes of N$\\sb{\\rm T}$ and <span class=\"etd-inline-math\">&beta;\\sp\\prime</span> occurring over 30 to 60 minutes. These observations support the view that Ca$\\sp{++}$-related mechanisms of regulation of N$\\sb{\\rm T}$ and <span class=\"etd-inline-math\">&beta;\\sp\\prime</span> are slow with respect to reported rates of increase of intracellular Ca$\\sp{++}$ and are thus incompatible with a direct effect of Ca$\\sp{++}$ on the epithelial Na$\\sp{+}$ channel.","abstract_has_math":true,"creators":["Kizer, Neil Lavern"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Physiology","degree_department":null,"school":null,"contributors":["Helman, Sandy I."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T12:53:01Z","date_published":"2011-05-07T12:53:01Z","updated_at":"2026-07-22T22:25:16Z","subjects":["Biology, Animal Physiology"],"languages":["eng"],"rights":["Copyright 1990 Kizer, Neil Lavern"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9114299","(UMI)AAI9114299"],"render_values":[{"text":"AAI9114299","href":null,"code":true},{"text":"(UMI)AAI9114299","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/20916","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Helman, Sandy I."]},{"key":"dc:creator","label":"Author","values":["Kizer, Neil Lavern"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T12:53:01Z","10000-01-01","1990"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Physiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Animal Physiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1990 Kizer, Neil Lavern"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9114299","(UMI)AAI9114299","http://hdl.handle.net/2142/20916"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Blocker-induced noise analysis of Na$\\sp{+}$ channels at apical membranes of intact and isolated epithelia of frog skin was carried out to investigate whether treatment of tissues with ionomycin, quinine, or acidification of basolateral solution shared similar mechanisms of inhibition of Na$\\sp{+}$ absorption. Experiments were done using staircase protocols (SC) that allowed evaluation of the dependence on blocker concentration of the macroscopic rates of Na$\\sp{+}$ transport I$\\sbsp{\\rm Na}{\\rm B}$, single channel Na$\\sp{+}$ current (i$\\sbsp{\\rm Na}{\\rm B}$), on and off blocker rate coefficients as well as determination of channel densities in open and blocked states and the Na$\\sp{+}$ channel open probability in the absence of blocker ($\\beta\\sp\\prime$). In addition to SC experiments, time course (TC) or paired time course (PTC) experiments allowed an assessment of the rates of change of the blocker rate coefficients and the changes of I$\\sbsp{\\rm Na}{\\rm B}$, i$\\sbsp{\\rm Na}{\\rm B}$, and the channel densities. Ionomycin, quinine, and acidification of the basolateral solution, despite their differing methods of increase of cytosolic Ca$\\sp{++}$ activity, caused in common inhibition of total channel density (N$\\sb{\\rm T}$) and stimulation of open channel density (N$\\sb{\\rm o}$) by way of increase of open channel probability ($\\beta\\sp\\prime$). Time course experiments revealed that inhibition of N$\\sb{\\rm T}$ and stimulation of $\\beta\\sp\\prime$ were delayed by several minutes, with relatively large changes of N$\\sb{\\rm T}$ and $\\beta\\sp\\prime$ occurring over 30 to 60 minutes. These observations support the view that Ca$\\sp{++}$-related mechanisms of regulation of N$\\sb{\\rm T}$ and $\\beta\\sp\\prime$ are slow with respect to reported rates of increase of intracellular Ca$\\sp{++}$ and are thus incompatible with a direct effect of Ca$\\sp{++}$ on the epithelial Na$\\sp{+}$ channel.","Made available in DSpace on 2011-05-07T12:53:01Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9114299.pdf: 2721308 bytes, checksum: b90a1f746a26245569e0b197cfdcdc02 (MD5) Previous issue date: 1990","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:47:10Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:21:18-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Calcium-dependent changes of apical membrane sodium channel density and open probability in frog skin"]}]}],"canonical_facts":{"dc:contributor":["Helman, Sandy I."],"dc:creator":["Kizer, Neil Lavern"],"dc:date":["2011-05-07T12:53:01Z","10000-01-01","1990"],"dc:description":["Blocker-induced noise analysis of Na$\\sp{+}$ channels at apical membranes of intact and isolated epithelia of frog skin was carried out to investigate whether treatment of tissues with ionomycin, quinine, or acidification of basolateral solution shared similar mechanisms of inhibition of Na$\\sp{+}$ absorption. Experiments were done using staircase protocols (SC) that allowed evaluation of the dependence on blocker concentration of the macroscopic rates of Na$\\sp{+}$ transport I$\\sbsp{\\rm Na}{\\rm B}$, single channel Na$\\sp{+}$ current (i$\\sbsp{\\rm Na}{\\rm B}$), on and off blocker rate coefficients as well as determination of channel densities in open and blocked states and the Na$\\sp{+}$ channel open probability in the absence of blocker ($\\beta\\sp\\prime$). In addition to SC experiments, time course (TC) or paired time course (PTC) experiments allowed an assessment of the rates of change of the blocker rate coefficients and the changes of I$\\sbsp{\\rm Na}{\\rm B}$, i$\\sbsp{\\rm Na}{\\rm B}$, and the channel densities. Ionomycin, quinine, and acidification of the basolateral solution, despite their differing methods of increase of cytosolic Ca$\\sp{++}$ activity, caused in common inhibition of total channel density (N$\\sb{\\rm T}$) and stimulation of open channel density (N$\\sb{\\rm o}$) by way of increase of open channel probability ($\\beta\\sp\\prime$). Time course experiments revealed that inhibition of N$\\sb{\\rm T}$ and stimulation of $\\beta\\sp\\prime$ were delayed by several minutes, with relatively large changes of N$\\sb{\\rm T}$ and $\\beta\\sp\\prime$ occurring over 30 to 60 minutes. These observations support the view that Ca$\\sp{++}$-related mechanisms of regulation of N$\\sb{\\rm T}$ and $\\beta\\sp\\prime$ are slow with respect to reported rates of increase of intracellular Ca$\\sp{++}$ and are thus incompatible with a direct effect of Ca$\\sp{++}$ on the epithelial Na$\\sp{+}$ channel.","Made available in DSpace on 2011-05-07T12:53:01Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9114299.pdf: 2721308 bytes, checksum: b90a1f746a26245569e0b197cfdcdc02 (MD5) Previous issue date: 1990","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:47:10Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:21:18-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9114299","(UMI)AAI9114299","http://hdl.handle.net/2142/20916"],"dc:language":["eng"],"dc:rights":["Copyright 1990 Kizer, Neil Lavern"],"dc:subject":["Biology, Animal Physiology"],"dc:title":["Calcium-dependent changes of apical membrane sodium channel density and open probability in frog skin"],"dc:type":["text"],"thesis:degree_discipline":["Physiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:16Z"}