{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/20904"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/20904","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Response of large intestinal mucosa in rats to dietary protein and fat","abstract":"Epidemiological studies show that colon cancer incidence is correlated with meat consumption, and high fecal pH. Reported here are studies which examine the hypothesis that ammonia is produced in greater quantities in the large intestine of rats when high protein diets are fed and that increased rates of cell proliferation of the colon mucosa are associated with the diets high in protein and fat. The objectives of this research were: (1) to determine the pH and ammonia concentrations of the contents in the cecum and different segments of colon in rats fed defined semi-purified diets and (2) to determine if the pH and ammonia concentrations of the cecal and colon contents are associated with differences in mucosal cell proliferation of the cecum and colon.","abstract_html":"Epidemiological studies show that colon cancer incidence is correlated with meat consumption, and high fecal pH. Reported here are studies which examine the hypothesis that ammonia is produced in greater quantities in the large intestine of rats when high protein diets are fed and that increased rates of cell proliferation of the colon mucosa are associated with the diets high in protein and fat. The objectives of this research were: (1) to determine the pH and ammonia concentrations of the contents in the cecum and different segments of colon in rats fed defined semi-purified diets and (2) to determine if the pH and ammonia concentrations of the cecal and colon contents are associated with differences in mucosal cell proliferation of the cecum and colon.","abstract_has_math":false,"creators":["Lin, Hsi-chiang"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Nutritional Sciences","degree_department":null,"school":null,"contributors":["Baker, David H."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T12:52:39Z","date_published":"2011-05-07T12:52:39Z","updated_at":"2026-07-22T22:25:16Z","subjects":["Agriculture, Food Science and Technology","Biology, Animal Physiology","Agriculture, Animal Culture and Nutrition"],"languages":["eng"],"rights":["Copyright 1990 Lin, Hsi-chiang"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9021719","(UMI)AAI9021719"],"render_values":[{"text":"AAI9021719","href":null,"code":true},{"text":"(UMI)AAI9021719","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/20904","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Baker, David H."]},{"key":"dc:creator","label":"Author","values":["Lin, Hsi-chiang"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T12:52:39Z","10000-01-01","1990"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Nutritional Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Agriculture, Food Science and Technology","Biology, Animal Physiology","Agriculture, Animal Culture and Nutrition"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1990 Lin, Hsi-chiang"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9021719","(UMI)AAI9021719","http://hdl.handle.net/2142/20904"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Epidemiological studies show that colon cancer incidence is correlated with meat consumption, and high fecal pH. Reported here are studies which examine the hypothesis that ammonia is produced in greater quantities in the large intestine of rats when high protein diets are fed and that increased rates of cell proliferation of the colon mucosa are associated with the diets high in protein and fat. The objectives of this research were: (1) to determine the pH and ammonia concentrations of the contents in the cecum and different segments of colon in rats fed defined semi-purified diets and (2) to determine if the pH and ammonia concentrations of the cecal and colon contents are associated with differences in mucosal cell proliferation of the cecum and colon.","Results of these studies showed the following: (1) Regional differences in dietary effects on intraluminal pH, ammonia concentration, water content and mucosal cell proliferation in the large intestine of rats. (2) High dietary protein significantly increased ammonia concentration along the large intestine, in vivo. (3) High dietary fat calories increased luminal pH in the colon of rats, in vivo. (4) Perfusion with ammonia containing solutions increased losses of mucus from the mucosal cells, increased DNA and carbohydrate losses into the perfusates, and produced damage to the mucosal epithelial cells. (5) Higher cell proliferative activities as measured by thymidine incorporation and ornithine decarboxylase (ODC) activity of mucosal cells in the distal colon were observed in rats consuming diets high in protein and fat calories.","It is concluded that ammonia arising from high protein intakes causes damage to mucosal cells which is followed by enhanced cell proliferation. Increased ODC activity and thymidine incorporation in the distal colon of rats fed high fat and protein diets is likely to result from high concentrations of unionized ammonia. Reassessment of the role of diet in colon cancer etiology should consider luminal pH, which influences ionization and concentration of the compounds in the colonic lumen which come into contact with mucosal cells.","Made available in DSpace on 2011-05-07T12:52:39Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9021719.pdf: 5023579 bytes, checksum: 92dd5d40aaa0366d444754b34af777ca (MD5) Previous issue date: 1990","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:47:05Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:21:13-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Response of large intestinal mucosa in rats to dietary protein and fat"]}]}],"canonical_facts":{"dc:contributor":["Baker, David H."],"dc:creator":["Lin, Hsi-chiang"],"dc:date":["2011-05-07T12:52:39Z","10000-01-01","1990"],"dc:description":["Epidemiological studies show that colon cancer incidence is correlated with meat consumption, and high fecal pH. Reported here are studies which examine the hypothesis that ammonia is produced in greater quantities in the large intestine of rats when high protein diets are fed and that increased rates of cell proliferation of the colon mucosa are associated with the diets high in protein and fat. The objectives of this research were: (1) to determine the pH and ammonia concentrations of the contents in the cecum and different segments of colon in rats fed defined semi-purified diets and (2) to determine if the pH and ammonia concentrations of the cecal and colon contents are associated with differences in mucosal cell proliferation of the cecum and colon.","Results of these studies showed the following: (1) Regional differences in dietary effects on intraluminal pH, ammonia concentration, water content and mucosal cell proliferation in the large intestine of rats. (2) High dietary protein significantly increased ammonia concentration along the large intestine, in vivo. (3) High dietary fat calories increased luminal pH in the colon of rats, in vivo. (4) Perfusion with ammonia containing solutions increased losses of mucus from the mucosal cells, increased DNA and carbohydrate losses into the perfusates, and produced damage to the mucosal epithelial cells. (5) Higher cell proliferative activities as measured by thymidine incorporation and ornithine decarboxylase (ODC) activity of mucosal cells in the distal colon were observed in rats consuming diets high in protein and fat calories.","It is concluded that ammonia arising from high protein intakes causes damage to mucosal cells which is followed by enhanced cell proliferation. Increased ODC activity and thymidine incorporation in the distal colon of rats fed high fat and protein diets is likely to result from high concentrations of unionized ammonia. Reassessment of the role of diet in colon cancer etiology should consider luminal pH, which influences ionization and concentration of the compounds in the colonic lumen which come into contact with mucosal cells.","Made available in DSpace on 2011-05-07T12:52:39Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9021719.pdf: 5023579 bytes, checksum: 92dd5d40aaa0366d444754b34af777ca (MD5) Previous issue date: 1990","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:47:05Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:21:13-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9021719","(UMI)AAI9021719","http://hdl.handle.net/2142/20904"],"dc:language":["eng"],"dc:rights":["Copyright 1990 Lin, Hsi-chiang"],"dc:subject":["Agriculture, Food Science and Technology","Biology, Animal Physiology","Agriculture, Animal Culture and Nutrition"],"dc:title":["Response of large intestinal mucosa in rats to dietary protein and fat"],"dc:type":["text"],"thesis:degree_discipline":["Nutritional Sciences"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:16Z"}