Hepatic Apo E mRNA levels in BALB/c and C57BL/6 mice fed different levels and types of oil
type: CreativeWork
description: Described here is the first known application of differential display to isolate genes regulated by level of dietary lipids in female BALB/c mice. Differences were revealed with all five primer pairs tested. Nine fragments were isolated, cloned, partially sequenced and compared to the Genbank data base. Sequence analyses identified Apolipoprotein E, P450-15-$\alpha$-hydroxylase (Cyp 2a4) and seven unique genes. Apolipoprotein E is found on most lipoproteins and binds primarily to the LDL and Apo E receptors in the liver, thereby clearing lipoproteins from the plasma. Cyp 2a4 hydroxylates testosterone, progesterone and androstenedione at the 15-$\alpha$ position for excretion. Northern analyses revealed that Cyp 2a4 was not regulated by our dietary treatments. Four feeding studies examined hepatic mRNA for Apo E in BALB/c mice fed diets varying in level and source of fat. All diets contained the same ratio of calories to other nutrients except carbohydrates and fat. Northern analyses of Apo E mRNA abundance in mice fed diets containing 0, 5, 10, 15 or 20% corn oil for 3 weeks confirmed that increasing dietary corn oil decreased abundance of Apo E in mice fed 20% corn oil compared to 5% (p $<$ 0.01). A third study investigated the hepatic mRNA level of Apo E in BALB/c and C57BL/6 mice fed semipurified diets containing 3 or 20% corn oil. Although differences were not statistically significant, C57BL/6 mice fed 20% corn oil showed increased expression of Apo E while BALB/c fed 20% showed decreased expression. A fourth study examined the relationship between hepatic Apo E mRNA levels and dietary fat level, serum lipid responses, stage of estrus and fast/refeeding. Mice were fed 3, 10 or 20% corn oil diets for 3 weeks. Northern analyses revealed that hepatic expression of Apo E mRNA was lower in mice fed 20% oil compared with 3% and 10% (p $<$ 0.0001) and was higher postprandially (p $<$ 0.0001). Serum triglycerides decreased with fasting and increased postprandially (p $…
abstract: Described here is the first known application of differential display to isolate genes regulated by level of dietary lipids in female BALB/c mice. Differences were revealed with all five primer pairs tested. Nine fragments were isolated, cloned, partially sequenced and compared to the Genbank data base. Sequence analyses identified Apolipoprotein E, P450-15-$\alpha$-hydroxylase (Cyp 2a4) and seven unique genes. Apolipoprotein E is found on most lipoproteins and binds primarily to the LDL and Apo E receptors in the liver, thereby clearing lipoproteins from the plasma. Cyp 2a4 hydroxylates testosterone, progesterone and androstenedione at the 15-$\alpha$ position for excretion. Northern analyses revealed that Cyp 2a4 was not regulated by our dietary treatments. Four feeding studies examined hepatic mRNA for Apo E in BALB/c mice fed diets varying in level and source of fat. All diets contained the same ratio of calories to other nutrients except carbohydrates and fat. Northern analyses of Apo E mRNA abundance in mice fed diets containing 0, 5, 10, 15 or 20% corn oil for 3 weeks confirmed that increasing dietary corn oil decreased abundance of Apo E in mice fed 20% corn oil compared to 5% (p $<$ 0.01). A third study investigated the hepatic mRNA level of Apo E in BALB/c and C57BL/6 mice fed semipurified diets containing 3 or 20% corn oil. Although differences were not statistically significant, C57BL/6 mice fed 20% corn oil showed increased expression of Apo E while BALB/c fed 20% showed decreased expression. A fourth study examined the relationship between hepatic Apo E mRNA levels and dietary fat level, serum lipid responses, stage of estrus and fast/refeeding. Mice were fed 3, 10 or 20% corn oil diets for 3 weeks. Northern analyses revealed that hepatic expression of Apo E mRNA was lower in mice fed 20% oil compared with 3% and 10% (p $<$ 0.0001) and was higher postprandially (p $<$ 0.0001). Serum triglycerides decreased with fasting and increased postprandially (p $…
author: Paisley, Elizabeth Ann
language: eng
keywords: Biology, Animal Physiology, Health Sciences, Nutrition
date published: 2011-05-07T12:51:45Z
date modified: 2026-07-22T22:25:16Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/20872