{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/20655"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/20655","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Characterization of genes involved in tabtoxinine-beta-lactam and lysine biosynthesis by Pseudomonas syringae pv. tabaci strain PTBR2.024","abstract":"U of I Only","abstract_html":"U of I Only","abstract_has_math":false,"creators":["Liu, Lixia"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Crop Sciences","degree_department":null,"school":null,"contributors":["Shaw, Paul D."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T12:45:30Z","date_published":"2011-05-07T12:45:30Z","updated_at":"2026-07-22T22:25:16Z","subjects":["Biology, Molecular","Biology, Microbiology"],"languages":["eng"],"rights":["Copyright 1996 Liu, Lixia"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["9780591199246","AAI9712359","(UMI)AAI9712359"],"render_values":[{"text":"9780591199246","href":null,"code":true},{"text":"AAI9712359","href":null,"code":true},{"text":"(UMI)AAI9712359","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/20655","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Shaw, Paul D."]},{"key":"dc:creator","label":"Author","values":["Liu, Lixia"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T12:45:30Z","10000-01-01","1996"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Crop Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Molecular","Biology, Microbiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1996 Liu, Lixia"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["9780591199246","AAI9712359","(UMI)AAI9712359","http://hdl.handle.net/2142/20655"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["U of I Only","The relationship between the biosynthesis of tabtoxinine-$\\beta$-lactam $\\rm(T\\beta L)$ and lysine by Pseudomonas syringae pv. tabaci strain BR2.024 (PTBR2.024) was investigated. A Tn5 mutant of PTBR2.024 showing diaminopimelate auxotrophy and $\\rm T\\beta L$-deficiency was isolated. Complementation results indicate that a region containing single open reading frame (ORF) is able to complement both mutant phenotypes. The deduced amino acid sequence of the ORF has a significant sequence homology to known dapB gene products, a lysine biosynthetic enzyme of bacteria. Furthermore, the ORF is able to complement an E. coli dapB mutant. The ORF was designated dapB. These results suggest that L-2,3,4,5-tetrahydrodipicolinate is a common intermediate in lysine and $\\rm T\\beta L$ biosynthesis.","A gene with a predicted product having an amino acid sequence homologous to known dapD gene products, another lysine biosynthetic enzyme in bacteria, was also identified. Complementation tests, in vitro transcription/translation analysis and enzymatic assays indicate that the dapD-like gene is able to encode a product with THDPA N-succinyltransferase (THDPA-ST) activity in E. coli. However, no THDPA-ST activity was detected in PTBR2.024 and a mutation in the dapD-like gene has no effect on lysine biosynthesis of strain PTBR2.024. These results indicate that the dapD-like gene is not involved in lysine biosynthesis in PTBR2.024. The mutation, however, caused a 50 percent reduction in $\\rm T\\beta L$ production. This suggests that the dapD-like gene may be involved in $\\rm T\\beta L$ biosynthesis. The dapD-like gene was named tabB.","Enzymatic assay results indicate that PTBR2.024 uses an acetyl pathway for lysine biosynthesis. This is the first report of a Gram-negative bacterium utilizing that pathway.","An gene, ORF$\\rm\\sb{L}$, able to complement the dapD mutant $\\beta$274 also was isolated from the PTBR2.024 genomic library. A mutant with an insertion in ORF$\\rm\\sb{L}$ produced as much T$\\beta$L as parent strain. However, the mutant had reduced growth rate in minimal medium. The growth rate was restored by addition of lysine in minimal medium. Complementation analyses indicate that the ORF$\\rm\\sb{L}$ mutant phenotype was due to a polar effect. This suggests that the ORF$\\rm\\sb{L}$ may not be involved in either lysine or tabtoxin biosynthesis.","Made available in DSpace on 2011-05-07T12:45:30Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9712359.pdf: 4202813 bytes, checksum: b7e83edefe978ec62f5941a48824423b (MD5) Previous issue date: 1996","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:45:22Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:20:05-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission"]},{"key":"dc:title","label":"Title","values":["Characterization of genes involved in tabtoxinine-beta-lactam and lysine biosynthesis by Pseudomonas syringae pv. tabaci strain PTBR2.024"]}]}],"canonical_facts":{"dc:contributor":["Shaw, Paul D."],"dc:creator":["Liu, Lixia"],"dc:date":["2011-05-07T12:45:30Z","10000-01-01","1996"],"dc:description":["U of I Only","The relationship between the biosynthesis of tabtoxinine-$\\beta$-lactam $\\rm(T\\beta L)$ and lysine by Pseudomonas syringae pv. tabaci strain BR2.024 (PTBR2.024) was investigated. A Tn5 mutant of PTBR2.024 showing diaminopimelate auxotrophy and $\\rm T\\beta L$-deficiency was isolated. Complementation results indicate that a region containing single open reading frame (ORF) is able to complement both mutant phenotypes. The deduced amino acid sequence of the ORF has a significant sequence homology to known dapB gene products, a lysine biosynthetic enzyme of bacteria. Furthermore, the ORF is able to complement an E. coli dapB mutant. The ORF was designated dapB. These results suggest that L-2,3,4,5-tetrahydrodipicolinate is a common intermediate in lysine and $\\rm T\\beta L$ biosynthesis.","A gene with a predicted product having an amino acid sequence homologous to known dapD gene products, another lysine biosynthetic enzyme in bacteria, was also identified. Complementation tests, in vitro transcription/translation analysis and enzymatic assays indicate that the dapD-like gene is able to encode a product with THDPA N-succinyltransferase (THDPA-ST) activity in E. coli. However, no THDPA-ST activity was detected in PTBR2.024 and a mutation in the dapD-like gene has no effect on lysine biosynthesis of strain PTBR2.024. These results indicate that the dapD-like gene is not involved in lysine biosynthesis in PTBR2.024. The mutation, however, caused a 50 percent reduction in $\\rm T\\beta L$ production. This suggests that the dapD-like gene may be involved in $\\rm T\\beta L$ biosynthesis. The dapD-like gene was named tabB.","Enzymatic assay results indicate that PTBR2.024 uses an acetyl pathway for lysine biosynthesis. This is the first report of a Gram-negative bacterium utilizing that pathway.","An gene, ORF$\\rm\\sb{L}$, able to complement the dapD mutant $\\beta$274 also was isolated from the PTBR2.024 genomic library. A mutant with an insertion in ORF$\\rm\\sb{L}$ produced as much T$\\beta$L as parent strain. However, the mutant had reduced growth rate in minimal medium. The growth rate was restored by addition of lysine in minimal medium. Complementation analyses indicate that the ORF$\\rm\\sb{L}$ mutant phenotype was due to a polar effect. This suggests that the ORF$\\rm\\sb{L}$ may not be involved in either lysine or tabtoxin biosynthesis.","Made available in DSpace on 2011-05-07T12:45:30Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9712359.pdf: 4202813 bytes, checksum: b7e83edefe978ec62f5941a48824423b (MD5) Previous issue date: 1996","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:45:22Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:20:05-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission"],"dc:identifier":["9780591199246","AAI9712359","(UMI)AAI9712359","http://hdl.handle.net/2142/20655"],"dc:language":["eng"],"dc:rights":["Copyright 1996 Liu, Lixia"],"dc:subject":["Biology, Molecular","Biology, Microbiology"],"dc:title":["Characterization of genes involved in tabtoxinine-beta-lactam and lysine biosynthesis by Pseudomonas syringae pv. tabaci strain PTBR2.024"],"dc:type":["text"],"thesis:degree_discipline":["Crop Sciences"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:16Z"}