{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/20589"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/20589","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Macrophage heterogeneity generated by differential gene expression","abstract":"Restriction data tranferred 2014-07-01T11:19:49-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","abstract_html":"Restriction data tranferred 2014-07-01T11:19:49-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","abstract_has_math":false,"creators":["Yang, Sung-Don"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Animal Sciences","degree_department":null,"school":null,"contributors":["Gaskins, H. Rex"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T12:43:34Z","date_published":"2011-05-07T12:43:34Z","updated_at":"2026-07-22T22:25:16Z","subjects":["Biology, Molecular","Biology, Cell"],"languages":["eng"],"rights":["Copyright 1995 Yang, Sung-Don"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9543779","(UMI)AAI9543779"],"render_values":[{"text":"AAI9543779","href":null,"code":true},{"text":"(UMI)AAI9543779","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/20589","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Gaskins, H. Rex"]},{"key":"dc:creator","label":"Author","values":["Yang, Sung-Don"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T12:43:34Z","10000-01-01","1995"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Animal Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Molecular","Biology, Cell"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1995 Yang, Sung-Don"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9543779","(UMI)AAI9543779","http://hdl.handle.net/2142/20589"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Restriction data tranferred 2014-07-01T11:19:49-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only","Macrophages (mos) exist as functionally heterogeneous populations. Mos derived from bone marrow precursor cells using either colony-stimulating factor-1 (CSF-1) or granulocyte/macrophage colony-stimulating factor (GM-CSF) have been studied to determine the distinct functional phenotypes both constitutively and after stimulation. In the present study, mo heterogeneity was examined by a subtraction cDNA library to identify genes differentially expressed by distinct mo populations constitutively and after activation by IFN-$\\gamma.$ Cultures of bone marrow-derived mos were treated with 50 U/ml IFN-$\\gamma$ for 40, 70 and 140 min to induce or enhance expression of early responsive genes regulated by IFN-$\\gamma,$ and these stimulated mos were pooled. Poly(A)$\\sp+$ RNA was prepared from both unactivated and IFN-$\\gamma$-stimulated mos, and cDNA libraries were constructed in ZAP-II. Genes commonly expressed by both untreated and activated mo populations were removed by subtraction using biotin-avidin precipitation of hybrid complexes. Further selection was performed using differential screening of cDNAs prepared from mRNA from unactivated mos, followed by colony hybridization to remove sister clones.","Of 31 clones randomly selected from the subtraction library, five clones showed intrinsic differential expression between GM-CSF- and CSF-1-derived mos, and another five clones revealed induced or enhanced expression by IFN-$\\gamma$ in GM-CSF-derived mos. In CSF-1-derived mos, these ten cDNA clones were either not expressed (GM2B1 and the small RNA species of GM3E1) or constitutively expressed (GM3A2, large mRNA species of GM3E1, GM4A12, GM2A12 and GM2B8). Four clones (GM3D7, GM1A2, GM1B4 and GM1F2) even showed reduced expression following IFN-$\\gamma$ stimulation in CSF-1-derived mos. These cDNAs, except GM2B1 and GM3A2, were not significantly homologous with known DNA sequences, implying that they represent novel cDNAs differentially expressed by distinct mo populations before or after treatment with IFN-$\\gamma.$ These data imply that the functional heterogeneity of bone marrow-derived mos (BMDMs) may be due to differential expression of as yet unidentified genes. Additional analysis of these cDNAs and their deduced amino acid sequences are discussed on the basis of the possible implication of these cDNAs in mo heterogeneity induced by different hematopoietic stimuli.","Made available in DSpace on 2011-05-07T12:43:34Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9543779.pdf: 6102074 bytes, checksum: cc7320ff52267e5b2c61131a181224cb (MD5) Previous issue date: 1995","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:44:54Z Item is restricted indefinitely."]},{"key":"dc:title","label":"Title","values":["Macrophage heterogeneity generated by differential gene expression"]}]}],"canonical_facts":{"dc:contributor":["Gaskins, H. Rex"],"dc:creator":["Yang, Sung-Don"],"dc:date":["2011-05-07T12:43:34Z","10000-01-01","1995"],"dc:description":["Restriction data tranferred 2014-07-01T11:19:49-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only","Macrophages (mos) exist as functionally heterogeneous populations. Mos derived from bone marrow precursor cells using either colony-stimulating factor-1 (CSF-1) or granulocyte/macrophage colony-stimulating factor (GM-CSF) have been studied to determine the distinct functional phenotypes both constitutively and after stimulation. In the present study, mo heterogeneity was examined by a subtraction cDNA library to identify genes differentially expressed by distinct mo populations constitutively and after activation by IFN-$\\gamma.$ Cultures of bone marrow-derived mos were treated with 50 U/ml IFN-$\\gamma$ for 40, 70 and 140 min to induce or enhance expression of early responsive genes regulated by IFN-$\\gamma,$ and these stimulated mos were pooled. Poly(A)$\\sp+$ RNA was prepared from both unactivated and IFN-$\\gamma$-stimulated mos, and cDNA libraries were constructed in ZAP-II. Genes commonly expressed by both untreated and activated mo populations were removed by subtraction using biotin-avidin precipitation of hybrid complexes. Further selection was performed using differential screening of cDNAs prepared from mRNA from unactivated mos, followed by colony hybridization to remove sister clones.","Of 31 clones randomly selected from the subtraction library, five clones showed intrinsic differential expression between GM-CSF- and CSF-1-derived mos, and another five clones revealed induced or enhanced expression by IFN-$\\gamma$ in GM-CSF-derived mos. In CSF-1-derived mos, these ten cDNA clones were either not expressed (GM2B1 and the small RNA species of GM3E1) or constitutively expressed (GM3A2, large mRNA species of GM3E1, GM4A12, GM2A12 and GM2B8). Four clones (GM3D7, GM1A2, GM1B4 and GM1F2) even showed reduced expression following IFN-$\\gamma$ stimulation in CSF-1-derived mos. These cDNAs, except GM2B1 and GM3A2, were not significantly homologous with known DNA sequences, implying that they represent novel cDNAs differentially expressed by distinct mo populations before or after treatment with IFN-$\\gamma.$ These data imply that the functional heterogeneity of bone marrow-derived mos (BMDMs) may be due to differential expression of as yet unidentified genes. Additional analysis of these cDNAs and their deduced amino acid sequences are discussed on the basis of the possible implication of these cDNAs in mo heterogeneity induced by different hematopoietic stimuli.","Made available in DSpace on 2011-05-07T12:43:34Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9543779.pdf: 6102074 bytes, checksum: cc7320ff52267e5b2c61131a181224cb (MD5) Previous issue date: 1995","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:44:54Z Item is restricted indefinitely."],"dc:identifier":["AAI9543779","(UMI)AAI9543779","http://hdl.handle.net/2142/20589"],"dc:language":["eng"],"dc:rights":["Copyright 1995 Yang, Sung-Don"],"dc:subject":["Biology, Molecular","Biology, Cell"],"dc:title":["Macrophage heterogeneity generated by differential gene expression"],"dc:type":["text"],"thesis:degree_discipline":["Animal Sciences"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:16Z"}