{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/20541"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/20541","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Solution structure and dynamics of myoglobin and its mutants","abstract":"This thesis discusses the solution structure and function of sperm whale myoglobin. The solution structure of sperm whale myoglobin, with atomic resolution is obtained by using nuclear magnetic resonance spectroscopy (NMR).","abstract_html":"This thesis discusses the solution structure and function of sperm whale myoglobin. The solution structure of sperm whale myoglobin, with atomic resolution is obtained by using nuclear magnetic resonance spectroscopy (NMR).","abstract_has_math":false,"creators":["Chiu, Mark Lawrence"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":["Sligar, Stephen G."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T12:42:13Z","date_published":"2011-05-07T12:42:13Z","updated_at":"2026-07-22T22:25:16Z","subjects":["Chemistry, Biochemistry"],"languages":["eng"],"rights":["Copyright 1992 Chiu, Mark Lawrence"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9215792","(UMI)AAI9215792"],"render_values":[{"text":"AAI9215792","href":null,"code":true},{"text":"(UMI)AAI9215792","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/20541","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Sligar, Stephen G."]},{"key":"dc:creator","label":"Author","values":["Chiu, Mark Lawrence"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T12:42:13Z","10000-01-01","1992"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Biochemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1992 Chiu, Mark Lawrence"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9215792","(UMI)AAI9215792","http://hdl.handle.net/2142/20541"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["This thesis discusses the solution structure and function of sperm whale myoglobin. The solution structure of sperm whale myoglobin, with atomic resolution is obtained by using nuclear magnetic resonance spectroscopy (NMR).","In solution, the protein samples a wider array of conformational substates. The carbon monoxide ligand has served as a model system for resolving the difference between equilibrium fluctuations and deterministic functionally important motions. By using C$\\sp{17}$O, one can study the interactions of the heme pocket residues and the heme ligand. An explanation for some of myoglobin's conformational substates lies in the possibility that the heme bound ligand can be bent or oriented differently with respect to the heme plane. From Oxygen-17 NMR studies, another explanation for the source of myoglobin's conformational heterogeneity emerges. This thesis presents an argument for the ability of the histidine 64, which has van der Waals contact with the heme-bound carbon monoxide, to tautomerize and flip with respect to its beta and gamma carbon axis. The different states and orientations of this histidine 64 can explain the earlier discussions of conformational heterogeneity.","Since the heme pocket of crystalline myoglobin appears to be solvent inaccessible to small ligands, some form of protein fluctuation must take place in order for ligands to enter and exit the heme pocket. By determining the hydrogen exchange rates of the backbone amide protons, a map of the flexibility of the protein is obtained. By correcting for local primary structure effects, the amide proton exchange rates also provide guides to the potential mechanism of ligand accessibility.","Site-directed mutagenesis on sperm whale myoglobin has permitted the alteration of the heme iron coordination scheme. The His64Tyr Mb mutant has a six-coordinate heme iron with four heme pyrroles, proximal histidine, and distal tyrosine as ligands. Since the pK of the binding of the tyrosine to the heme iron is at 5.0, there is a possibility that the tyrosine group is binding the heme iron as a phenol group. X-ray absorption at the near edge, optical, and electron spin resonance spectroscopies have been used to show that the tyrosine may bind to the heme iron as a phenol group. (Abstract shortened with permission of author.)","Made available in DSpace on 2011-05-07T12:42:13Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9215792.pdf: 12202782 bytes, checksum: bb1dc6f46184dd7372981b9774ea7546 (MD5) Previous issue date: 1992","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:44:35Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:19:38-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Solution structure and dynamics of myoglobin and its mutants"]}]}],"canonical_facts":{"dc:contributor":["Sligar, Stephen G."],"dc:creator":["Chiu, Mark Lawrence"],"dc:date":["2011-05-07T12:42:13Z","10000-01-01","1992"],"dc:description":["This thesis discusses the solution structure and function of sperm whale myoglobin. The solution structure of sperm whale myoglobin, with atomic resolution is obtained by using nuclear magnetic resonance spectroscopy (NMR).","In solution, the protein samples a wider array of conformational substates. The carbon monoxide ligand has served as a model system for resolving the difference between equilibrium fluctuations and deterministic functionally important motions. By using C$\\sp{17}$O, one can study the interactions of the heme pocket residues and the heme ligand. An explanation for some of myoglobin's conformational substates lies in the possibility that the heme bound ligand can be bent or oriented differently with respect to the heme plane. From Oxygen-17 NMR studies, another explanation for the source of myoglobin's conformational heterogeneity emerges. This thesis presents an argument for the ability of the histidine 64, which has van der Waals contact with the heme-bound carbon monoxide, to tautomerize and flip with respect to its beta and gamma carbon axis. The different states and orientations of this histidine 64 can explain the earlier discussions of conformational heterogeneity.","Since the heme pocket of crystalline myoglobin appears to be solvent inaccessible to small ligands, some form of protein fluctuation must take place in order for ligands to enter and exit the heme pocket. By determining the hydrogen exchange rates of the backbone amide protons, a map of the flexibility of the protein is obtained. By correcting for local primary structure effects, the amide proton exchange rates also provide guides to the potential mechanism of ligand accessibility.","Site-directed mutagenesis on sperm whale myoglobin has permitted the alteration of the heme iron coordination scheme. The His64Tyr Mb mutant has a six-coordinate heme iron with four heme pyrroles, proximal histidine, and distal tyrosine as ligands. Since the pK of the binding of the tyrosine to the heme iron is at 5.0, there is a possibility that the tyrosine group is binding the heme iron as a phenol group. X-ray absorption at the near edge, optical, and electron spin resonance spectroscopies have been used to show that the tyrosine may bind to the heme iron as a phenol group. (Abstract shortened with permission of author.)","Made available in DSpace on 2011-05-07T12:42:13Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9215792.pdf: 12202782 bytes, checksum: bb1dc6f46184dd7372981b9774ea7546 (MD5) Previous issue date: 1992","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:44:35Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:19:38-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9215792","(UMI)AAI9215792","http://hdl.handle.net/2142/20541"],"dc:language":["eng"],"dc:rights":["Copyright 1992 Chiu, Mark Lawrence"],"dc:subject":["Chemistry, Biochemistry"],"dc:title":["Solution structure and dynamics of myoglobin and its mutants"],"dc:type":["text"],"thesis:degree_discipline":["Biochemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:16Z"}