{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/20534"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/20534","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"The macrophage activating properties of growth hormone in vitro and in vivo: Comparison to interferon-gamma (IF N-gamma)","abstract":"\"Macrophages (M$\\Phi$) elicited by injections of inflammatory agents or obtained from animals infected with intracellular parasites are primed so that they respond to phagocytosis of opsonized particles such as opsonized-zymosan (Op-zym) with a marked increase in the respiratory burst, NADPH oxidase system. This capacity to increase the release of reactive oxygen metabolites, including superoxide anion (O$\\sb2\\sp-$), plays an important role in the enhanced microbicidal capacity of activated M$\\Phi$. To date, Interferon-$\\gamma$ (IFN-$\\gamma$), a classically defined macrophage activating factor (MAF), has been the most completely characterized substance both in vitro and in vivo known to be produced by mammalian cells that augments the production of reactive oxygen metabolites, including O$\\sb2\\sp-$, by M$\\Phi$. Other MAF-like molecules such as Interleukin-4 and Granulocyte/Macrophage Colony Stimulating Factor are thought to exist. Growth hormone (GH) is synthesized by the adenohypophysis, and it has also been shown to be produced by activated lymphoid cells. Therefore, the role of GH in regulating M$\\Phi$ activation has been addressed in this dissertation. Data are presented which provide the first evidence that GH augments the production of O$\\sb2\\sp-$ by mononuclear phagocytes in vitro and in vivo. When either native or recombinant GH were administered to hypophysectomized rats in vivo, activation of peritoneal M$\\Phi$, as measured by O$\\sb2\\sp-$ release, was equivalent to that of M$\\Phi$ from rats primed with IFN-$\\gamma$. Because M$\\Phi$ are capable of exerting antimicrobial activities toward intracellular pathogens through an enhanced respiratory burst in vitro and in vivo, data are presented which indicate that rats treated with IFN-$\\gamma$ or GH have enhanced resistance to a Salmonella typhimurium infection over a 14-day period and this resistance was due in-part to the generation of reactive oxygen intermediates. Since it is important to know whether GH augments other M$\\Phi$ functional activities, other studies address the ability of IFN-$\\gamma$ or GH administered to hypophysectomized rats in vivo to up-regulate the cytokine Tumor Necrosis Factor-$\\alpha$. The biological relevance of this observation is demonstrated by showing that in vivo priming of endotoxin induced hemorrhagic necrosis of murine tumors in mice can be mediated by IFN-$\\gamma$ and GH. In summary, the data presented in these experiments suggest that GH plays an important \"\"cytokine-like\"\" role in regulating M$\\Phi$ functional activities in vitro and in vivo.\"","abstract_html":"&quot;Macrophages (M$\\Phi$) elicited by injections of inflammatory agents or obtained from animals infected with intracellular parasites are primed so that they respond to phagocytosis of opsonized particles such as opsonized-zymosan (Op-zym) with a marked increase in the respiratory burst, NADPH oxidase system. This capacity to increase the release of reactive oxygen metabolites, including superoxide anion (O$\\sb2\\sp-$), plays an important role in the enhanced microbicidal capacity of activated M$\\Phi$. To date, Interferon-<span class=\"etd-inline-math\">&gamma;</span> (IFN-<span class=\"etd-inline-math\">&gamma;</span>), a classically defined macrophage activating factor (MAF), has been the most completely characterized substance both in vitro and in vivo known to be produced by mammalian cells that augments the production of reactive oxygen metabolites, including O$\\sb2\\sp-$, by M$\\Phi$. Other MAF-like molecules such as Interleukin-4 and Granulocyte/Macrophage Colony Stimulating Factor are thought to exist. Growth hormone (GH) is synthesized by the adenohypophysis, and it has also been shown to be produced by activated lymphoid cells. Therefore, the role of GH in regulating M$\\Phi$ activation has been addressed in this dissertation. Data are presented which provide the first evidence that GH augments the production of O$\\sb2\\sp-$ by mononuclear phagocytes in vitro and in vivo. When either native or recombinant GH were administered to hypophysectomized rats in vivo, activation of peritoneal M$\\Phi$, as measured by O$\\sb2\\sp-$ release, was equivalent to that of M$\\Phi$ from rats primed with IFN-<span class=\"etd-inline-math\">&gamma;</span>. Because M$\\Phi$ are capable of exerting antimicrobial activities toward intracellular pathogens through an enhanced respiratory burst in vitro and in vivo, data are presented which indicate that rats treated with IFN-<span class=\"etd-inline-math\">&gamma;</span> or GH have enhanced resistance to a Salmonella typhimurium infection over a 14-day period and this resistance was due in-part to the generation of reactive oxygen intermediates. Since it is important to know whether GH augments other M$\\Phi$ functional activities, other studies address the ability of IFN-<span class=\"etd-inline-math\">&gamma;</span> or GH administered to hypophysectomized rats in vivo to up-regulate the cytokine Tumor Necrosis Factor-<span class=\"etd-inline-math\">&alpha;</span>. The biological relevance of this observation is demonstrated by showing that in vivo priming of endotoxin induced hemorrhagic necrosis of murine tumors in mice can be mediated by IFN-<span class=\"etd-inline-math\">&gamma;</span> and GH. In summary, the data presented in these experiments suggest that GH plays an important &quot;&quot;cytokine-like&quot;&quot; role in regulating M$\\Phi$ functional activities in vitro and in vivo.&quot;","abstract_has_math":true,"creators":["Edwards, Carl Keith, III"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Animal Sciences","degree_department":null,"school":null,"contributors":["Kelley, Keith W."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T12:41:59Z","date_published":"2011-05-07T12:41:59Z","updated_at":"2026-07-22T22:25:16Z","subjects":["Health Sciences, Immunology"],"languages":["eng"],"rights":["Copyright 1989 Edwards, Carl Keith, III"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI8924810","(UMI)AAI8924810"],"render_values":[{"text":"AAI8924810","href":null,"code":true},{"text":"(UMI)AAI8924810","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/20534","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kelley, Keith W."]},{"key":"dc:creator","label":"Author","values":["Edwards, Carl Keith, III"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T12:41:59Z","10000-01-01","1989"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Animal Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Health Sciences, Immunology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1989 Edwards, Carl Keith, III"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI8924810","(UMI)AAI8924810","http://hdl.handle.net/2142/20534"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["\"Macrophages (M$\\Phi$) elicited by injections of inflammatory agents or obtained from animals infected with intracellular parasites are primed so that they respond to phagocytosis of opsonized particles such as opsonized-zymosan (Op-zym) with a marked increase in the respiratory burst, NADPH oxidase system. This capacity to increase the release of reactive oxygen metabolites, including superoxide anion (O$\\sb2\\sp-$), plays an important role in the enhanced microbicidal capacity of activated M$\\Phi$. To date, Interferon-$\\gamma$ (IFN-$\\gamma$), a classically defined macrophage activating factor (MAF), has been the most completely characterized substance both in vitro and in vivo known to be produced by mammalian cells that augments the production of reactive oxygen metabolites, including O$\\sb2\\sp-$, by M$\\Phi$. Other MAF-like molecules such as Interleukin-4 and Granulocyte/Macrophage Colony Stimulating Factor are thought to exist. Growth hormone (GH) is synthesized by the adenohypophysis, and it has also been shown to be produced by activated lymphoid cells. Therefore, the role of GH in regulating M$\\Phi$ activation has been addressed in this dissertation. Data are presented which provide the first evidence that GH augments the production of O$\\sb2\\sp-$ by mononuclear phagocytes in vitro and in vivo. When either native or recombinant GH were administered to hypophysectomized rats in vivo, activation of peritoneal M$\\Phi$, as measured by O$\\sb2\\sp-$ release, was equivalent to that of M$\\Phi$ from rats primed with IFN-$\\gamma$. Because M$\\Phi$ are capable of exerting antimicrobial activities toward intracellular pathogens through an enhanced respiratory burst in vitro and in vivo, data are presented which indicate that rats treated with IFN-$\\gamma$ or GH have enhanced resistance to a Salmonella typhimurium infection over a 14-day period and this resistance was due in-part to the generation of reactive oxygen intermediates. Since it is important to know whether GH augments other M$\\Phi$ functional activities, other studies address the ability of IFN-$\\gamma$ or GH administered to hypophysectomized rats in vivo to up-regulate the cytokine Tumor Necrosis Factor-$\\alpha$. The biological relevance of this observation is demonstrated by showing that in vivo priming of endotoxin induced hemorrhagic necrosis of murine tumors in mice can be mediated by IFN-$\\gamma$ and GH. In summary, the data presented in these experiments suggest that GH plays an important \"\"cytokine-like\"\" role in regulating M$\\Phi$ functional activities in vitro and in vivo.\"","Made available in DSpace on 2011-05-07T12:41:59Z (GMT). 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This capacity to increase the release of reactive oxygen metabolites, including superoxide anion (O$\\sb2\\sp-$), plays an important role in the enhanced microbicidal capacity of activated M$\\Phi$. To date, Interferon-$\\gamma$ (IFN-$\\gamma$), a classically defined macrophage activating factor (MAF), has been the most completely characterized substance both in vitro and in vivo known to be produced by mammalian cells that augments the production of reactive oxygen metabolites, including O$\\sb2\\sp-$, by M$\\Phi$. Other MAF-like molecules such as Interleukin-4 and Granulocyte/Macrophage Colony Stimulating Factor are thought to exist. Growth hormone (GH) is synthesized by the adenohypophysis, and it has also been shown to be produced by activated lymphoid cells. Therefore, the role of GH in regulating M$\\Phi$ activation has been addressed in this dissertation. Data are presented which provide the first evidence that GH augments the production of O$\\sb2\\sp-$ by mononuclear phagocytes in vitro and in vivo. When either native or recombinant GH were administered to hypophysectomized rats in vivo, activation of peritoneal M$\\Phi$, as measured by O$\\sb2\\sp-$ release, was equivalent to that of M$\\Phi$ from rats primed with IFN-$\\gamma$. Because M$\\Phi$ are capable of exerting antimicrobial activities toward intracellular pathogens through an enhanced respiratory burst in vitro and in vivo, data are presented which indicate that rats treated with IFN-$\\gamma$ or GH have enhanced resistance to a Salmonella typhimurium infection over a 14-day period and this resistance was due in-part to the generation of reactive oxygen intermediates. Since it is important to know whether GH augments other M$\\Phi$ functional activities, other studies address the ability of IFN-$\\gamma$ or GH administered to hypophysectomized rats in vivo to up-regulate the cytokine Tumor Necrosis Factor-$\\alpha$. The biological relevance of this observation is demonstrated by showing that in vivo priming of endotoxin induced hemorrhagic necrosis of murine tumors in mice can be mediated by IFN-$\\gamma$ and GH. In summary, the data presented in these experiments suggest that GH plays an important \"\"cytokine-like\"\" role in regulating M$\\Phi$ functional activities in vitro and in vivo.\"","Made available in DSpace on 2011-05-07T12:41:59Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 8924810.pdf: 9107257 bytes, checksum: ccd2d42b77cbaf744e7eca305e694c5e (MD5) Previous issue date: 1989","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:44:32Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:19:37-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI8924810","(UMI)AAI8924810","http://hdl.handle.net/2142/20534"],"dc:language":["eng"],"dc:rights":["Copyright 1989 Edwards, Carl Keith, III"],"dc:subject":["Health Sciences, Immunology"],"dc:title":["The macrophage activating properties of growth hormone in vitro and in vivo: Comparison to interferon-gamma (IF N-gamma)"],"dc:type":["text"],"thesis:degree_discipline":["Animal Sciences"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:16Z"}