Identification, quantitation and immunogenicity of culture-derived soluble Babesia bigemina antigens
Soluble Babesia bigemina antigens were prepared from short-term in vitro cultures utilizing a microaerophilous stationary phase culture system. In two pilot immunization experiments involving 6 cattle, 3 doses of 25 ml-equivalent (838 EIA units) and 2 doses of 10 ml-equivalent (529 EIA units), respectively, of the crude culture-derived antigens administered with 1 mg of saponin (Quil-A) adjuvant, evoked primary and secondary antibody responses as detected and measured by the indirect fluorescent antibody (IFA) test and immunodiffusion tests.
University of Illinois - Urbana-Champaign
Networked Digital Library of Theses and Dissertations
University of Illinois at Urbana-Champaign