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It was found that this $\\alpha$-purple, nonsulfur photosynthetic bacterium carries two different circular chromosomes whose sizes are 3046 $\\pm$ 95 and 914 $\\pm$ 17 kb in addition to five endogenous plasmids with a total size of approximately 450 kb.","abstract_html":"Physical mapping techniques were employed to construct both a physical and genetic maps of the Rhodobacter sphaeroides 2.4.1 genome. It was found that this <span class=\"etd-inline-math\">&alpha;</span>-purple, nonsulfur photosynthetic bacterium carries two different circular chromosomes whose sizes are 3046 $\\pm$ 95 and 914 $\\pm$ 17 kb in addition to five endogenous plasmids with a total size of approximately 450 kb.","abstract_has_math":true,"creators":["Suwanto, Antonius"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":["Salyers, Abigail A."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T12:38:51Z","date_published":"2011-05-07T12:38:51Z","updated_at":"2026-07-22T22:25:15Z","subjects":["Biology, Molecular","Biology, Genetics","Biology, Microbiology"],"languages":["eng"],"rights":["Copyright 1992 Suwanto, Antonius"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9215894","(UMI)AAI9215894"],"render_values":[{"text":"AAI9215894","href":null,"code":true},{"text":"(UMI)AAI9215894","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/20426","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Salyers, Abigail A."]},{"key":"dc:creator","label":"Author","values":["Suwanto, Antonius"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T12:38:51Z","10000-01-01","1992"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Molecular","Biology, Genetics","Biology, Microbiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1992 Suwanto, Antonius"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9215894","(UMI)AAI9215894","http://hdl.handle.net/2142/20426"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Physical mapping techniques were employed to construct both a physical and genetic maps of the Rhodobacter sphaeroides 2.4.1 genome. It was found that this $\\alpha$-purple, nonsulfur photosynthetic bacterium carries two different circular chromosomes whose sizes are 3046 $\\pm$ 95 and 914 $\\pm$ 17 kb in addition to five endogenous plasmids with a total size of approximately 450 kb.","The smallest, 42 kb, endogenous plasmid was observed to be self transmissible. As little as an 425 bp oriT-containing DNA fragment isolated from this plasmid was able to confer transfer, between R. sphaeroides strains, when it was cloned into an otherwise non-transmissible plasmid. The DNA sequence of this oriT containing fragment reveals an A-T rich region, several direct and inverted repeats, as well as putative IHF-binding sites.","Random insertion of oriT into the R. sphaeroides 2.4.1 genome generated Hfr-like strains which exhibit a gradient of marker transfer, and has further provided classical genetic evidence for the circularity as well as the presence of two distinct chromosomal linkage groups in this bacterium.","Made available in DSpace on 2011-05-07T12:38:51Z (GMT). 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It was found that this $\\alpha$-purple, nonsulfur photosynthetic bacterium carries two different circular chromosomes whose sizes are 3046 $\\pm$ 95 and 914 $\\pm$ 17 kb in addition to five endogenous plasmids with a total size of approximately 450 kb.","The smallest, 42 kb, endogenous plasmid was observed to be self transmissible. As little as an 425 bp oriT-containing DNA fragment isolated from this plasmid was able to confer transfer, between R. sphaeroides strains, when it was cloned into an otherwise non-transmissible plasmid. The DNA sequence of this oriT containing fragment reveals an A-T rich region, several direct and inverted repeats, as well as putative IHF-binding sites.","Random insertion of oriT into the R. sphaeroides 2.4.1 genome generated Hfr-like strains which exhibit a gradient of marker transfer, and has further provided classical genetic evidence for the circularity as well as the presence of two distinct chromosomal linkage groups in this bacterium.","Made available in DSpace on 2011-05-07T12:38:51Z (GMT). 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