Transcriptional regulatory elements for basal expression and effects of phenobarbital and dexamethasone onmRNA levels of rabbit CYP2C genes
Cytochrome P450 (CYP) genes, expressed in many species, are involved in the oxidative metabolism of many xenobiotis as well as endogenous compounds. Expression of the rabbit CYP2C1 gene is observed only after induction with phenobarbital and is confined to the liver. The CYP2C2 gene is inducible in both liver and kidney. In order to identify the DNA domains involved in the regulation of general as well as hepatic-specific transcription, 5$\sp\prime$-flanking fragments from CYP2C1 (C1) and CYP2C2 (C2) genes were inserted in front of the firefly luciferase reporter gene and analyzed for promoter activity in HepG2 (hepatic) and COS-1 (non-hepatic) cells. Maximum basal expression in HepG2 and COS-1 cells was observed when the constructions contained C1 and C2 gene fragments that extended to $-$2000 and $-$410 bp (base pairs), respectively.
Biology, Molecular, Biology, Cell, Biology, Animal Physiology
University of Illinois - Urbana-Champaign
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University of Illinois at Urbana-Champaign