Continuous flow fast atom bombardment and electrospray ionization mass spectrometry in natural products research
Continuous flow fast atom bombardment (CF-FAB) has been applied as a liquid chromatography/mass spectrometry (LC/MS) and liquid chromatography/mass spectrometry/mass spectrometry (LC/MS/MS) interface in natural products research. Initial studies concentrated on characterization of the interface, optimization of operational parameters and sensitivity assessment. Using a CF-FAB interface, femtomole quantities of different classes of compounds were detected, which is two orders of magnitude more sensitive than by standard FAB. Later, CF-FAB was applied to the analysis of didemnin, streptovaricin, and ecteinascidin mixtures in isocratic and/or gradient modes.
University of Illinois - Urbana-Champaign
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University of Illinois at Urbana-Champaign