{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/19964"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/19964","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Localization of P450 aromatase in sperm and estrogen receptors in the epididymal region of the rooster","abstract":"The epididymal region of the rooster (rete testis, efferent ductules, and epididymal duct) has an important role in the production of fertile sperm by controlling the luminal microenvironment required for sperm maturation. Even though androgen is the major hormone involved in regulating functions of the epididymal region, there are many other regulatory molecules in testicular fluid that appear to play a role. The presence of estrogen receptors (ER) in the male reproductive tract suggests that estrogen may be important for epididymal functions. This investigation was undertaken to test the hypothesis that estrogen has a role in the regulation of the male reproductive system of the rooster. Specific objectives of my dissertation were: (1) to determine if germ cells and epididymal sperm are sources of estrogen; (2) to determine if the epididymal region of the rooster is a target for estrogen; and (3) to determine if testicular steroids regulate the expression of ER in the epididymal region of the rooster. The major findings were: (1) adult rooster testicular germ cells and epididymal sperm contain active P450 aromatase (P450arom) which converts androgens to estrogens; (2) ER and ER mRNA are present in the epididymal region of the rooster, which suggests that this region is a target for estrogen; and (3) steady state levels of ER mRNA in the epididymal region of roosters was suppressed in the presence of reduced serum concentrations of testosterone. These data clearly demonstrate that rooster germ cells and epididymal sperm are sites for the synthesis of estrogen and the epididymal region of the rooster is a target for estrogen. Preliminary studies suggest that testicular steroids may regulate the expression of ER in the epididymal region of the rooster. The presence of estrogen as well as ER in the epididymal region suggests that estrogen might be involved in the regulation of epididymal functions in the rooster.","abstract_html":"The epididymal region of the rooster (rete testis, efferent ductules, and epididymal duct) has an important role in the production of fertile sperm by controlling the luminal microenvironment required for sperm maturation. Even though androgen is the major hormone involved in regulating functions of the epididymal region, there are many other regulatory molecules in testicular fluid that appear to play a role. The presence of estrogen receptors (ER) in the male reproductive tract suggests that estrogen may be important for epididymal functions. This investigation was undertaken to test the hypothesis that estrogen has a role in the regulation of the male reproductive system of the rooster. Specific objectives of my dissertation were: (1) to determine if germ cells and epididymal sperm are sources of estrogen; (2) to determine if the epididymal region of the rooster is a target for estrogen; and (3) to determine if testicular steroids regulate the expression of ER in the epididymal region of the rooster. The major findings were: (1) adult rooster testicular germ cells and epididymal sperm contain active P450 aromatase (P450arom) which converts androgens to estrogens; (2) ER and ER mRNA are present in the epididymal region of the rooster, which suggests that this region is a target for estrogen; and (3) steady state levels of ER mRNA in the epididymal region of roosters was suppressed in the presence of reduced serum concentrations of testosterone. These data clearly demonstrate that rooster germ cells and epididymal sperm are sites for the synthesis of estrogen and the epididymal region of the rooster is a target for estrogen. Preliminary studies suggest that testicular steroids may regulate the expression of ER in the epididymal region of the rooster. The presence of estrogen as well as ER in the epididymal region suggests that estrogen might be involved in the regulation of epididymal functions in the rooster.","abstract_has_math":false,"creators":["Kwon, Seok"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Animal Sciences","degree_department":null,"school":null,"contributors":["Bahr, Janice M."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T12:24:21Z","date_published":"2011-05-07T12:24:21Z","updated_at":"2026-07-22T22:25:15Z","subjects":["Biology, Cell","Biology, Animal Physiology"],"languages":["eng"],"rights":["Copyright 1996 Kwon, Seok"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["9780591088137","AAI9702570","(UMI)AAI9702570"],"render_values":[{"text":"9780591088137","href":null,"code":true},{"text":"AAI9702570","href":null,"code":true},{"text":"(UMI)AAI9702570","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/19964","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Bahr, Janice M."]},{"key":"dc:creator","label":"Author","values":["Kwon, Seok"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T12:24:21Z","10000-01-01","1996"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Animal Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Cell","Biology, Animal Physiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1996 Kwon, Seok"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["9780591088137","AAI9702570","(UMI)AAI9702570","http://hdl.handle.net/2142/19964"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The epididymal region of the rooster (rete testis, efferent ductules, and epididymal duct) has an important role in the production of fertile sperm by controlling the luminal microenvironment required for sperm maturation. Even though androgen is the major hormone involved in regulating functions of the epididymal region, there are many other regulatory molecules in testicular fluid that appear to play a role. The presence of estrogen receptors (ER) in the male reproductive tract suggests that estrogen may be important for epididymal functions. This investigation was undertaken to test the hypothesis that estrogen has a role in the regulation of the male reproductive system of the rooster. Specific objectives of my dissertation were: (1) to determine if germ cells and epididymal sperm are sources of estrogen; (2) to determine if the epididymal region of the rooster is a target for estrogen; and (3) to determine if testicular steroids regulate the expression of ER in the epididymal region of the rooster. The major findings were: (1) adult rooster testicular germ cells and epididymal sperm contain active P450 aromatase (P450arom) which converts androgens to estrogens; (2) ER and ER mRNA are present in the epididymal region of the rooster, which suggests that this region is a target for estrogen; and (3) steady state levels of ER mRNA in the epididymal region of roosters was suppressed in the presence of reduced serum concentrations of testosterone. These data clearly demonstrate that rooster germ cells and epididymal sperm are sites for the synthesis of estrogen and the epididymal region of the rooster is a target for estrogen. Preliminary studies suggest that testicular steroids may regulate the expression of ER in the epididymal region of the rooster. The presence of estrogen as well as ER in the epididymal region suggests that estrogen might be involved in the regulation of epididymal functions in the rooster.","Made available in DSpace on 2011-05-07T12:24:21Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9702570.pdf: 4448382 bytes, checksum: 6863df25d9eeb2364413adb8688eb822 (MD5) Previous issue date: 1996","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:40:37Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:17:26-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Localization of P450 aromatase in sperm and estrogen receptors in the epididymal region of the rooster"]}]}],"canonical_facts":{"dc:contributor":["Bahr, Janice M."],"dc:creator":["Kwon, Seok"],"dc:date":["2011-05-07T12:24:21Z","10000-01-01","1996"],"dc:description":["The epididymal region of the rooster (rete testis, efferent ductules, and epididymal duct) has an important role in the production of fertile sperm by controlling the luminal microenvironment required for sperm maturation. Even though androgen is the major hormone involved in regulating functions of the epididymal region, there are many other regulatory molecules in testicular fluid that appear to play a role. The presence of estrogen receptors (ER) in the male reproductive tract suggests that estrogen may be important for epididymal functions. This investigation was undertaken to test the hypothesis that estrogen has a role in the regulation of the male reproductive system of the rooster. Specific objectives of my dissertation were: (1) to determine if germ cells and epididymal sperm are sources of estrogen; (2) to determine if the epididymal region of the rooster is a target for estrogen; and (3) to determine if testicular steroids regulate the expression of ER in the epididymal region of the rooster. The major findings were: (1) adult rooster testicular germ cells and epididymal sperm contain active P450 aromatase (P450arom) which converts androgens to estrogens; (2) ER and ER mRNA are present in the epididymal region of the rooster, which suggests that this region is a target for estrogen; and (3) steady state levels of ER mRNA in the epididymal region of roosters was suppressed in the presence of reduced serum concentrations of testosterone. These data clearly demonstrate that rooster germ cells and epididymal sperm are sites for the synthesis of estrogen and the epididymal region of the rooster is a target for estrogen. Preliminary studies suggest that testicular steroids may regulate the expression of ER in the epididymal region of the rooster. The presence of estrogen as well as ER in the epididymal region suggests that estrogen might be involved in the regulation of epididymal functions in the rooster.","Made available in DSpace on 2011-05-07T12:24:21Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9702570.pdf: 4448382 bytes, checksum: 6863df25d9eeb2364413adb8688eb822 (MD5) Previous issue date: 1996","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:40:37Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:17:26-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["9780591088137","AAI9702570","(UMI)AAI9702570","http://hdl.handle.net/2142/19964"],"dc:language":["eng"],"dc:rights":["Copyright 1996 Kwon, Seok"],"dc:subject":["Biology, Cell","Biology, Animal Physiology"],"dc:title":["Localization of P450 aromatase in sperm and estrogen receptors in the epididymal region of the rooster"],"dc:type":["text"],"thesis:degree_discipline":["Animal Sciences"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:15Z"}