{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/19946"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/19946","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Molecular ecology of plant cell wall hydrolysis by mixed cultures of Ruminococcus albus 8, Ruminococcus flavefaciens FD-1 and Fibrobacter succinogenes S85","abstract":"A total of 6 specific oligonucleotide probes were designed for the Ruminococci, 3 targeted to sites on 16S rRNA of Ruminococcus albus and 3 targeted to sites on 16S rRNA of Ruminococcus flavefaciens. A subspecies probe for Fibrobacter succinogenes S85 (Stahl et al., 1988) and a eubacterial kingdom probe (Stahl and Amann, 1991) also were used. Bacteria were grown as monocultures, dicultures and in triculture on cellobiose, cellulose and alkaline hydrogen peroxide-treated wheat straw to study competition among the three bacterial species on these substrates. The cellubiose-grown cultures were incubated at 39$\\sp\\circ$C for 10 hours and sampled hourly. The cellulose and alkaline hydrogen peroxide-treated wheat straw-grown cultures were incubated at 39$\\sp\\circ$C for 70 hours and sampled every 8 hours. Ribosomal RNA was extracted from all the samples, blotted on nylon membranes and hybridized with labelled $\\sp{32}$P probes. The membranes the were exposed to films. Results showed that R. albus 8 inhibited the growth of R. flavefaciens FD-1 when grown as a diculture on cellubiose or cellulose. However, R. albus 8 was outnumbered by F. succinogenes S85 on cellobiose but not on cellulose. R. flavefaciens FD-1 outnumbered F. succinogenes S85 on both cellobiose and cellulose. In the triculture, R. flavefaciens FD-1 was inhibited, R. albus 8 appeared to dominate during early incubation (12-48 hours) on cellulose, while F. succinogenes S85 became predominant during the late incubation phase (60-70 hours). On alkaline hydrogen peroxide-treated wheat straw, F. succinogenes S85 grew better than either R. albus 8 or R. flavefaciens FD-1. However, R. flavefaciens FD-1 was present in low numbers throughout the incubation period unlike on cellobiose and cellulose. The results on cellobiose and cellulose suggest that R. albus 8 produced a substance that inhibited the growth of R. flavefaciens FD-1.","abstract_html":"A total of 6 specific oligonucleotide probes were designed for the Ruminococci, 3 targeted to sites on 16S rRNA of Ruminococcus albus and 3 targeted to sites on 16S rRNA of Ruminococcus flavefaciens. A subspecies probe for Fibrobacter succinogenes S85 (Stahl et al., 1988) and a eubacterial kingdom probe (Stahl and Amann, 1991) also were used. Bacteria were grown as monocultures, dicultures and in triculture on cellobiose, cellulose and alkaline hydrogen peroxide-treated wheat straw to study competition among the three bacterial species on these substrates. The cellubiose-grown cultures were incubated at 39$\\sp\\circ$C for 10 hours and sampled hourly. The cellulose and alkaline hydrogen peroxide-treated wheat straw-grown cultures were incubated at 39$\\sp\\circ$C for 70 hours and sampled every 8 hours. Ribosomal RNA was extracted from all the samples, blotted on nylon membranes and hybridized with labelled $\\sp{32}$P probes. The membranes the were exposed to films. Results showed that R. albus 8 inhibited the growth of R. flavefaciens FD-1 when grown as a diculture on cellubiose or cellulose. However, R. albus 8 was outnumbered by F. succinogenes S85 on cellobiose but not on cellulose. R. flavefaciens FD-1 outnumbered F. succinogenes S85 on both cellobiose and cellulose. In the triculture, R. flavefaciens FD-1 was inhibited, R. albus 8 appeared to dominate during early incubation (12-48 hours) on cellulose, while F. succinogenes S85 became predominant during the late incubation phase (60-70 hours). On alkaline hydrogen peroxide-treated wheat straw, F. succinogenes S85 grew better than either R. albus 8 or R. flavefaciens FD-1. However, R. flavefaciens FD-1 was present in low numbers throughout the incubation period unlike on cellobiose and cellulose. The results on cellobiose and cellulose suggest that R. albus 8 produced a substance that inhibited the growth of R. flavefaciens FD-1.","abstract_has_math":true,"creators":["Odenyo, Agnes Awino"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Animal Sciences","degree_department":null,"school":null,"contributors":["Mackie, Roderick I."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T12:23:49Z","date_published":"2011-05-07T12:23:49Z","updated_at":"2026-07-22T22:25:15Z","subjects":["Biology, Ecology","Biology, Microbiology","Agriculture, Plant Pathology"],"languages":["eng"],"rights":["Copyright 1992 Odenyo, Agnes Awino"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9305642","(UMI)AAI9305642"],"render_values":[{"text":"AAI9305642","href":null,"code":true},{"text":"(UMI)AAI9305642","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/19946","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Mackie, Roderick I."]},{"key":"dc:creator","label":"Author","values":["Odenyo, Agnes Awino"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T12:23:49Z","10000-01-01","1992"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Animal Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Ecology","Biology, Microbiology","Agriculture, Plant Pathology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1992 Odenyo, Agnes Awino"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9305642","(UMI)AAI9305642","http://hdl.handle.net/2142/19946"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["A total of 6 specific oligonucleotide probes were designed for the Ruminococci, 3 targeted to sites on 16S rRNA of Ruminococcus albus and 3 targeted to sites on 16S rRNA of Ruminococcus flavefaciens. A subspecies probe for Fibrobacter succinogenes S85 (Stahl et al., 1988) and a eubacterial kingdom probe (Stahl and Amann, 1991) also were used. Bacteria were grown as monocultures, dicultures and in triculture on cellobiose, cellulose and alkaline hydrogen peroxide-treated wheat straw to study competition among the three bacterial species on these substrates. The cellubiose-grown cultures were incubated at 39$\\sp\\circ$C for 10 hours and sampled hourly. The cellulose and alkaline hydrogen peroxide-treated wheat straw-grown cultures were incubated at 39$\\sp\\circ$C for 70 hours and sampled every 8 hours. Ribosomal RNA was extracted from all the samples, blotted on nylon membranes and hybridized with labelled $\\sp{32}$P probes. The membranes the were exposed to films. Results showed that R. albus 8 inhibited the growth of R. flavefaciens FD-1 when grown as a diculture on cellubiose or cellulose. However, R. albus 8 was outnumbered by F. succinogenes S85 on cellobiose but not on cellulose. R. flavefaciens FD-1 outnumbered F. succinogenes S85 on both cellobiose and cellulose. In the triculture, R. flavefaciens FD-1 was inhibited, R. albus 8 appeared to dominate during early incubation (12-48 hours) on cellulose, while F. succinogenes S85 became predominant during the late incubation phase (60-70 hours). On alkaline hydrogen peroxide-treated wheat straw, F. succinogenes S85 grew better than either R. albus 8 or R. flavefaciens FD-1. However, R. flavefaciens FD-1 was present in low numbers throughout the incubation period unlike on cellobiose and cellulose. The results on cellobiose and cellulose suggest that R. albus 8 produced a substance that inhibited the growth of R. flavefaciens FD-1.","Made available in DSpace on 2011-05-07T12:23:49Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9305642.pdf: 6144968 bytes, checksum: 4fce9e7b1b5dfae2b101be12124131d6 (MD5) Previous issue date: 1992","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:40:30Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:17:23-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Molecular ecology of plant cell wall hydrolysis by mixed cultures of Ruminococcus albus 8, Ruminococcus flavefaciens FD-1 and Fibrobacter succinogenes S85"]}]}],"canonical_facts":{"dc:contributor":["Mackie, Roderick I."],"dc:creator":["Odenyo, Agnes Awino"],"dc:date":["2011-05-07T12:23:49Z","10000-01-01","1992"],"dc:description":["A total of 6 specific oligonucleotide probes were designed for the Ruminococci, 3 targeted to sites on 16S rRNA of Ruminococcus albus and 3 targeted to sites on 16S rRNA of Ruminococcus flavefaciens. A subspecies probe for Fibrobacter succinogenes S85 (Stahl et al., 1988) and a eubacterial kingdom probe (Stahl and Amann, 1991) also were used. Bacteria were grown as monocultures, dicultures and in triculture on cellobiose, cellulose and alkaline hydrogen peroxide-treated wheat straw to study competition among the three bacterial species on these substrates. The cellubiose-grown cultures were incubated at 39$\\sp\\circ$C for 10 hours and sampled hourly. The cellulose and alkaline hydrogen peroxide-treated wheat straw-grown cultures were incubated at 39$\\sp\\circ$C for 70 hours and sampled every 8 hours. Ribosomal RNA was extracted from all the samples, blotted on nylon membranes and hybridized with labelled $\\sp{32}$P probes. The membranes the were exposed to films. Results showed that R. albus 8 inhibited the growth of R. flavefaciens FD-1 when grown as a diculture on cellubiose or cellulose. However, R. albus 8 was outnumbered by F. succinogenes S85 on cellobiose but not on cellulose. R. flavefaciens FD-1 outnumbered F. succinogenes S85 on both cellobiose and cellulose. In the triculture, R. flavefaciens FD-1 was inhibited, R. albus 8 appeared to dominate during early incubation (12-48 hours) on cellulose, while F. succinogenes S85 became predominant during the late incubation phase (60-70 hours). On alkaline hydrogen peroxide-treated wheat straw, F. succinogenes S85 grew better than either R. albus 8 or R. flavefaciens FD-1. However, R. flavefaciens FD-1 was present in low numbers throughout the incubation period unlike on cellobiose and cellulose. The results on cellobiose and cellulose suggest that R. albus 8 produced a substance that inhibited the growth of R. flavefaciens FD-1.","Made available in DSpace on 2011-05-07T12:23:49Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9305642.pdf: 6144968 bytes, checksum: 4fce9e7b1b5dfae2b101be12124131d6 (MD5) Previous issue date: 1992","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:40:30Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:17:23-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9305642","(UMI)AAI9305642","http://hdl.handle.net/2142/19946"],"dc:language":["eng"],"dc:rights":["Copyright 1992 Odenyo, Agnes Awino"],"dc:subject":["Biology, Ecology","Biology, Microbiology","Agriculture, Plant Pathology"],"dc:title":["Molecular ecology of plant cell wall hydrolysis by mixed cultures of Ruminococcus albus 8, Ruminococcus flavefaciens FD-1 and Fibrobacter succinogenes S85"],"dc:type":["text"],"thesis:degree_discipline":["Animal Sciences"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:15Z"}