{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/19713"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/19713","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Characterization of a T cell receptor and its ligands","abstract":"Cytotoxic T lymphocytes (CTL) recognize and lyse cells presenting viral or tumor-associated antigens associated with class I major histocompatibility complex proteins. Recognition occurs between the T cell receptor (TCR) and a complex of an antigenic peptide/major histocompatibility complex (MHC) product. In addition, the CD8 molecule of the CTL stabilizes the interaction by binding the constant domain of the MHC product. Clone 2C recognizes a peptide (p2C) bound to the MHC product L$\\sp{\\rm d}.$ L$\\sp{\\rm d}$ levels expressed by a panel of tumor cells directly correlated with their ability to be lysed and incubation of target cells with exogenous p2C led to increased lysis by CTL 2C which was inhibited minimally with anti-CD8 or anti-TCR antibodies. Thus at high ligand densities, very few TCR molecules/2C cell are needed for recognition.","abstract_html":"Cytotoxic T lymphocytes (CTL) recognize and lyse cells presenting viral or tumor-associated antigens associated with class I major histocompatibility complex proteins. Recognition occurs between the T cell receptor (TCR) and a complex of an antigenic peptide/major histocompatibility complex (MHC) product. In addition, the CD8 molecule of the CTL stabilizes the interaction by binding the constant domain of the MHC product. Clone 2C recognizes a peptide (p2C) bound to the MHC product L$\\sp{\\rm d}.$ L$\\sp{\\rm d}$ levels expressed by a panel of tumor cells directly correlated with their ability to be lysed and incubation of target cells with exogenous p2C led to increased lysis by CTL 2C which was inhibited minimally with anti-CD8 or anti-TCR antibodies. Thus at high ligand densities, very few TCR molecules/2C cell are needed for recognition.","abstract_has_math":true,"creators":["Soo Hoo, William"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":["Kranz, David M."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T12:16:11Z","date_published":"2011-05-07T12:16:11Z","updated_at":"2026-07-22T22:25:14Z","subjects":["Biology, Molecular","Biology, Cell","Health Sciences, Immunology"],"languages":["eng"],"rights":["Copyright 1993 Soo Hoo, William"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9314942","(UMI)AAI9314942"],"render_values":[{"text":"AAI9314942","href":null,"code":true},{"text":"(UMI)AAI9314942","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/19713","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kranz, David M."]},{"key":"dc:creator","label":"Author","values":["Soo Hoo, William"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T12:16:11Z","10000-01-01","1993"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Molecular","Biology, Cell","Health Sciences, Immunology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1993 Soo Hoo, William"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9314942","(UMI)AAI9314942","http://hdl.handle.net/2142/19713"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Cytotoxic T lymphocytes (CTL) recognize and lyse cells presenting viral or tumor-associated antigens associated with class I major histocompatibility complex proteins. Recognition occurs between the T cell receptor (TCR) and a complex of an antigenic peptide/major histocompatibility complex (MHC) product. In addition, the CD8 molecule of the CTL stabilizes the interaction by binding the constant domain of the MHC product. Clone 2C recognizes a peptide (p2C) bound to the MHC product L$\\sp{\\rm d}.$ L$\\sp{\\rm d}$ levels expressed by a panel of tumor cells directly correlated with their ability to be lysed and incubation of target cells with exogenous p2C led to increased lysis by CTL 2C which was inhibited minimally with anti-CD8 or anti-TCR antibodies. Thus at high ligand densities, very few TCR molecules/2C cell are needed for recognition.","It has been observed previously that CTL bearing particular V$\\beta$ chains recognize microbial enterotoxins bound to class II MHC products. Clone 2C as well as other CTL derived from polyclonal T cell populations were able to recognize staphylococcal enterotoxin B (SEB) bound to class II-expressing target cells. Recognition was inhibited by anti-TCR, LFA-1, and CD8 antibodies but not by antibodies against other cell surface determinants. The striking thing about these results is that, contrary to previous studies, CD8 is required by CTL for recognizing SEB-labeled target cells. Using polyclonal CTL, we have shown that this observation is also influenced by ligand density. Taken together, we propose that CD8 function is essential for virtually all CTL.","A further study of CTL function requires the clarification of the binding properties of the TCR. The problem has been the difficulty in obtaining sufficient quantities of active receptor. To this end, a single-chain T cell receptor gene encoding the $\\rm V\\alpha/V\\beta$ domains of 2C was expressed in E. coli linked by a flexible polypeptide. The refolded $\\rm V\\alpha V\\beta$ protein was bound by anti-V$\\beta$8 and anti-TCR antibodies, suggesting the protein assumes a native conformation. These single-chain proteins will be useful as immunogens for eliciting anti-TCR antibodies and in the study of TCR structure and function.","Made available in DSpace on 2011-05-07T12:16:11Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9314942.pdf: 5963836 bytes, checksum: cd9d9d55f7288e1414d2fca613e7d3df (MD5) Previous issue date: 1993","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:38:55Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:16:18-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Characterization of a T cell receptor and its ligands"]}]}],"canonical_facts":{"dc:contributor":["Kranz, David M."],"dc:creator":["Soo Hoo, William"],"dc:date":["2011-05-07T12:16:11Z","10000-01-01","1993"],"dc:description":["Cytotoxic T lymphocytes (CTL) recognize and lyse cells presenting viral or tumor-associated antigens associated with class I major histocompatibility complex proteins. Recognition occurs between the T cell receptor (TCR) and a complex of an antigenic peptide/major histocompatibility complex (MHC) product. In addition, the CD8 molecule of the CTL stabilizes the interaction by binding the constant domain of the MHC product. Clone 2C recognizes a peptide (p2C) bound to the MHC product L$\\sp{\\rm d}.$ L$\\sp{\\rm d}$ levels expressed by a panel of tumor cells directly correlated with their ability to be lysed and incubation of target cells with exogenous p2C led to increased lysis by CTL 2C which was inhibited minimally with anti-CD8 or anti-TCR antibodies. Thus at high ligand densities, very few TCR molecules/2C cell are needed for recognition.","It has been observed previously that CTL bearing particular V$\\beta$ chains recognize microbial enterotoxins bound to class II MHC products. Clone 2C as well as other CTL derived from polyclonal T cell populations were able to recognize staphylococcal enterotoxin B (SEB) bound to class II-expressing target cells. Recognition was inhibited by anti-TCR, LFA-1, and CD8 antibodies but not by antibodies against other cell surface determinants. The striking thing about these results is that, contrary to previous studies, CD8 is required by CTL for recognizing SEB-labeled target cells. Using polyclonal CTL, we have shown that this observation is also influenced by ligand density. Taken together, we propose that CD8 function is essential for virtually all CTL.","A further study of CTL function requires the clarification of the binding properties of the TCR. The problem has been the difficulty in obtaining sufficient quantities of active receptor. To this end, a single-chain T cell receptor gene encoding the $\\rm V\\alpha/V\\beta$ domains of 2C was expressed in E. coli linked by a flexible polypeptide. The refolded $\\rm V\\alpha V\\beta$ protein was bound by anti-V$\\beta$8 and anti-TCR antibodies, suggesting the protein assumes a native conformation. These single-chain proteins will be useful as immunogens for eliciting anti-TCR antibodies and in the study of TCR structure and function.","Made available in DSpace on 2011-05-07T12:16:11Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9314942.pdf: 5963836 bytes, checksum: cd9d9d55f7288e1414d2fca613e7d3df (MD5) Previous issue date: 1993","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:38:55Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:16:18-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9314942","(UMI)AAI9314942","http://hdl.handle.net/2142/19713"],"dc:language":["eng"],"dc:rights":["Copyright 1993 Soo Hoo, William"],"dc:subject":["Biology, Molecular","Biology, Cell","Health Sciences, Immunology"],"dc:title":["Characterization of a T cell receptor and its ligands"],"dc:type":["text"],"thesis:degree_discipline":["Biochemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:14Z"}