Lateral organization and dynamics of phospholipids in membrane
Studies of the lateral organization and dynamics of phospholipids were carried out using fluorescence techniques and enzymatic methods. Using resonance energy transfer, no specific interactions were observed between gramicidin and dansyl-labeled phosphatidylcholine, phosphatidylethanolamine, or phosphatidic acid. However, another integral membrane protein, D-$\beta$-hydroxybutyrate dehydrogenase (BDH), reorganized the phospholipids in the bilayer into a nonrandom distribution. Although the enzyme has a specific requirement for phosphatidylcholine for activity, the extent of energy transfer decreased in the order phosphatidic acid, phosphatidylcholine, phosphatidylethanolamine.
University of Illinois - Urbana-Champaign
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University of Illinois at Urbana-Champaign