{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/19433"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/19433","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Capillary electrophoresis with wavelength-resolved fluorescence for analysis of peptides","abstract":"Wavelength-resolved fluorescence detection in capillary electrophoresis yields a powerful analytical tool for analysis of complex micro-environments. The design, development, and applications of wavelength-resolved fluorescence detection for capillary electrophoresis are reviewed.","abstract_html":"Wavelength-resolved fluorescence detection in capillary electrophoresis yields a powerful analytical tool for analysis of complex micro-environments. The design, development, and applications of wavelength-resolved fluorescence detection for capillary electrophoresis are reviewed.","abstract_has_math":false,"creators":["Timperman, Aaron Thomas"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Sweedler, Jonathan V."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T12:07:21Z","date_published":"2011-05-07T12:07:21Z","updated_at":"2026-07-22T22:25:12Z","subjects":["Chemistry, Analytical"],"languages":["eng"],"rights":["Copyright 1995 Timperman, Aaron Thomas"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9624517","(UMI)AAI9624517"],"render_values":[{"text":"AAI9624517","href":null,"code":true},{"text":"(UMI)AAI9624517","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/19433","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Sweedler, Jonathan V."]},{"key":"dc:creator","label":"Author","values":["Timperman, Aaron Thomas"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T12:07:21Z","10000-01-01","1995"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Analytical"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1995 Timperman, Aaron Thomas"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9624517","(UMI)AAI9624517","http://hdl.handle.net/2142/19433"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Wavelength-resolved fluorescence detection in capillary electrophoresis yields a powerful analytical tool for analysis of complex micro-environments. The design, development, and applications of wavelength-resolved fluorescence detection for capillary electrophoresis are reviewed.","A multichannel laser-induced fluorescence detector for capillary electrophoresis is described. The detection system combines yoctomole limits of detection with the simultaneous acquisition of entire fluorescence emission spectra. An Ar/Kr mixed gas ion laser provides great flexibility in excitation wavelengths and a holographic grating and charge-coupled device detector (CCD) combination allows a 500-nm spectral window to be acquired with 2-nm resolution. The limits of detection (LODs) are $5\\times10\\sp{-14}$ M or 80 molecules for sulforhodamine 101, and $1.5\\times10\\sp{-13}$ M Or 220 molecules for fluorescein in a 50 $\\mu$m I.D. capillary. An electropherogram of a mixture of amino acids derivatized with both Bodipy 503/512 C$\\sb3$ and Bodipy 576/589 C$\\sb3$ demonstrates that the analytes can be differentiated on the basis of both emission characteristics and migration times.","A native fluorescence detection system for capillary electrophoresis is described that achieves low attomole detection limits and simultaneous acquisition of complete fluorescence emission spectra. The detection system employs a frequency doubled krypton laser operating at 284 nm for excitation, a sheath flow cell, a reflective f/1.2 microscope objective, an imaging spectrograph, and a CCD detector. The detection capabilities are characterized with tryptophan and tyrosine which have limits of detection (3s) of $2\\times10\\sp{-10}$ M and, $2\\times10\\sp{-8}$ M, respectively. Acquisition of the fluorescence emission spectrum provides the ability to distinguish three classes of peptides: those that contain tryptophan, tyrosine, or both tryptophan and tyrosine.","The combination of wavelength-resolved fluorescence detection and a fluorescent pH indicator, incorporated into the running buffer, is used as a novel on-column pH monitor. When the outlet vial is removed, rapid pH shifts of more than 3 units are observed for running buffers in the 4-8 pH range. A front of reverse migrating OH$\\sp{-}$, and zones of stacking and depletion of the SNARF, pH indicator, are observed.","Made available in DSpace on 2011-05-07T12:07:21Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9624517.pdf: 3993700 bytes, checksum: 382b89f3acc9b269d5e760cf2868181b (MD5) Previous issue date: 1995","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:36:55Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:15:04-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Capillary electrophoresis with wavelength-resolved fluorescence for analysis of peptides"]}]}],"canonical_facts":{"dc:contributor":["Sweedler, Jonathan V."],"dc:creator":["Timperman, Aaron Thomas"],"dc:date":["2011-05-07T12:07:21Z","10000-01-01","1995"],"dc:description":["Wavelength-resolved fluorescence detection in capillary electrophoresis yields a powerful analytical tool for analysis of complex micro-environments. The design, development, and applications of wavelength-resolved fluorescence detection for capillary electrophoresis are reviewed.","A multichannel laser-induced fluorescence detector for capillary electrophoresis is described. The detection system combines yoctomole limits of detection with the simultaneous acquisition of entire fluorescence emission spectra. An Ar/Kr mixed gas ion laser provides great flexibility in excitation wavelengths and a holographic grating and charge-coupled device detector (CCD) combination allows a 500-nm spectral window to be acquired with 2-nm resolution. The limits of detection (LODs) are $5\\times10\\sp{-14}$ M or 80 molecules for sulforhodamine 101, and $1.5\\times10\\sp{-13}$ M Or 220 molecules for fluorescein in a 50 $\\mu$m I.D. capillary. An electropherogram of a mixture of amino acids derivatized with both Bodipy 503/512 C$\\sb3$ and Bodipy 576/589 C$\\sb3$ demonstrates that the analytes can be differentiated on the basis of both emission characteristics and migration times.","A native fluorescence detection system for capillary electrophoresis is described that achieves low attomole detection limits and simultaneous acquisition of complete fluorescence emission spectra. The detection system employs a frequency doubled krypton laser operating at 284 nm for excitation, a sheath flow cell, a reflective f/1.2 microscope objective, an imaging spectrograph, and a CCD detector. The detection capabilities are characterized with tryptophan and tyrosine which have limits of detection (3s) of $2\\times10\\sp{-10}$ M and, $2\\times10\\sp{-8}$ M, respectively. Acquisition of the fluorescence emission spectrum provides the ability to distinguish three classes of peptides: those that contain tryptophan, tyrosine, or both tryptophan and tyrosine.","The combination of wavelength-resolved fluorescence detection and a fluorescent pH indicator, incorporated into the running buffer, is used as a novel on-column pH monitor. When the outlet vial is removed, rapid pH shifts of more than 3 units are observed for running buffers in the 4-8 pH range. A front of reverse migrating OH$\\sp{-}$, and zones of stacking and depletion of the SNARF, pH indicator, are observed.","Made available in DSpace on 2011-05-07T12:07:21Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9624517.pdf: 3993700 bytes, checksum: 382b89f3acc9b269d5e760cf2868181b (MD5) Previous issue date: 1995","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:36:55Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:15:04-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9624517","(UMI)AAI9624517","http://hdl.handle.net/2142/19433"],"dc:language":["eng"],"dc:rights":["Copyright 1995 Timperman, Aaron Thomas"],"dc:subject":["Chemistry, Analytical"],"dc:title":["Capillary electrophoresis with wavelength-resolved fluorescence for analysis of peptides"],"dc:type":["text"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:12Z"}