{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/19391"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/19391","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Effects of individual fatty acids on cellular lipid synthesis, lipoprotein secretion, and receptor-mediated lipoprotein uptake in Hep-G2 cells","abstract":"Restriction data tranferred 2014-07-01T11:14:52-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","abstract_html":"Restriction data tranferred 2014-07-01T11:14:52-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","abstract_has_math":false,"creators":["Dokko, Ryowon Choue"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Nutritional Sciences","degree_department":null,"school":null,"contributors":["Cho, B.H."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T12:06:07Z","date_published":"2011-05-07T12:06:07Z","updated_at":"2026-07-22T22:25:12Z","subjects":["Health Sciences, Nutrition"],"languages":["eng"],"rights":["Copyright 1993 Dokko, Ryowon Choue"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9411604","(UMI)AAI9411604"],"render_values":[{"text":"AAI9411604","href":null,"code":true},{"text":"(UMI)AAI9411604","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/19391","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Cho, B.H."]},{"key":"dc:creator","label":"Author","values":["Dokko, Ryowon Choue"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T12:06:07Z","10000-01-01","1993"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Nutritional Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Health Sciences, Nutrition"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1993 Dokko, Ryowon Choue"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9411604","(UMI)AAI9411604","http://hdl.handle.net/2142/19391"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Restriction data tranferred 2014-07-01T11:14:52-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only","Effects of individual fatty acids on lipoprotein synthesis and secretion were studied in Hep-G2 cells. The cellular uptake rate of 18:0, 18:1, 18:2 and 18:3 was very similar. The synthesis of triglyceride was significantly increased by fatty acids inclusion; triglyceride synthesis was highest with 18:0 followed by 18:1, 18:2 and 18:3 and secretion was highest with 18:1 followed by 18:2, 18:3 and 18:0.","The synthesis and secretion of phospholipid was significantly increased by fatty acid inclusion. No comparative effect was found among fatty acids tested with an exception of 18:3 which has the lowest effect on phospholipid secretion. $\\sp{14}$C-acetate incorporation into cellular and medium cholesterol was significantly higher in all fatty acid groups compared to that of the control. For cholesterol secretion no significant difference among fatty acids tested was noted. However, 18:3 caused least cholesterol synthesis when it was compared with 18:1.","The inclusion of fatty acids showed notable changes in the fatty acid compositions of the cellular and medium lipids, mainly reflective the specific fatty acid added.","Although the incorporation of $\\sp3$H-leucine into cellular or medium protein (d $>$ 1.21 g/ml) was not affected by addition of fatty acids the incorporation of radioactivity into apolipoprotein was significantly increased (d $<$ 1.21 g/ml).","All fatty acids stimulated the incorporation of $\\sp3$H-leucine into apolipoprotein B. However, there was no significant change in the incorporation of $\\sp3$H-leucine into Apo E, Apo A-I and Apo C.","The exposure of Hep-G2 cells to $\\sp{125}$I-LDL in the medium resulted in a rapid internalization of LDL into the cell. Hep-G2 cells internalized $\\sp{125}$I-LDL via two pathways, a saturable, high affinity pathway and an unsaturable, low-affinity pathway.","All fatty acids significantly increased LDL binding and internalization of $\\sp{125}$I-LDL. Among different fatty acids, stearic acid caused least uptake of LDL. The addition of fatty acids caused significantly increased degradation of $\\sp{125}$I-labeled LDL. The amount of accumulated acid-soluble materials in the medium was least in stearic acid group.","Made available in DSpace on 2011-05-07T12:06:07Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9411604.pdf: 6259298 bytes, checksum: c4d73b8793808ed84208d8db637c4d0d (MD5) Previous issue date: 1993","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:36:38Z Item is restricted indefinitely."]},{"key":"dc:title","label":"Title","values":["Effects of individual fatty acids on cellular lipid synthesis, lipoprotein secretion, and receptor-mediated lipoprotein uptake in Hep-G2 cells"]}]}],"canonical_facts":{"dc:contributor":["Cho, B.H."],"dc:creator":["Dokko, Ryowon Choue"],"dc:date":["2011-05-07T12:06:07Z","10000-01-01","1993"],"dc:description":["Restriction data tranferred 2014-07-01T11:14:52-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only","Effects of individual fatty acids on lipoprotein synthesis and secretion were studied in Hep-G2 cells. The cellular uptake rate of 18:0, 18:1, 18:2 and 18:3 was very similar. The synthesis of triglyceride was significantly increased by fatty acids inclusion; triglyceride synthesis was highest with 18:0 followed by 18:1, 18:2 and 18:3 and secretion was highest with 18:1 followed by 18:2, 18:3 and 18:0.","The synthesis and secretion of phospholipid was significantly increased by fatty acid inclusion. No comparative effect was found among fatty acids tested with an exception of 18:3 which has the lowest effect on phospholipid secretion. $\\sp{14}$C-acetate incorporation into cellular and medium cholesterol was significantly higher in all fatty acid groups compared to that of the control. For cholesterol secretion no significant difference among fatty acids tested was noted. However, 18:3 caused least cholesterol synthesis when it was compared with 18:1.","The inclusion of fatty acids showed notable changes in the fatty acid compositions of the cellular and medium lipids, mainly reflective the specific fatty acid added.","Although the incorporation of $\\sp3$H-leucine into cellular or medium protein (d $>$ 1.21 g/ml) was not affected by addition of fatty acids the incorporation of radioactivity into apolipoprotein was significantly increased (d $<$ 1.21 g/ml).","All fatty acids stimulated the incorporation of $\\sp3$H-leucine into apolipoprotein B. However, there was no significant change in the incorporation of $\\sp3$H-leucine into Apo E, Apo A-I and Apo C.","The exposure of Hep-G2 cells to $\\sp{125}$I-LDL in the medium resulted in a rapid internalization of LDL into the cell. Hep-G2 cells internalized $\\sp{125}$I-LDL via two pathways, a saturable, high affinity pathway and an unsaturable, low-affinity pathway.","All fatty acids significantly increased LDL binding and internalization of $\\sp{125}$I-LDL. Among different fatty acids, stearic acid caused least uptake of LDL. The addition of fatty acids caused significantly increased degradation of $\\sp{125}$I-labeled LDL. The amount of accumulated acid-soluble materials in the medium was least in stearic acid group.","Made available in DSpace on 2011-05-07T12:06:07Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9411604.pdf: 6259298 bytes, checksum: c4d73b8793808ed84208d8db637c4d0d (MD5) Previous issue date: 1993","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:36:38Z Item is restricted indefinitely."],"dc:identifier":["AAI9411604","(UMI)AAI9411604","http://hdl.handle.net/2142/19391"],"dc:language":["eng"],"dc:rights":["Copyright 1993 Dokko, Ryowon Choue"],"dc:subject":["Health Sciences, Nutrition"],"dc:title":["Effects of individual fatty acids on cellular lipid synthesis, lipoprotein secretion, and receptor-mediated lipoprotein uptake in Hep-G2 cells"],"dc:type":["text"],"thesis:degree_discipline":["Nutritional Sciences"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:12Z"}