{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/19294"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/19294","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"The mechanism of selectivity and site of action of clomazone in tolerant-soybean and susceptible-cotton photomixotrophic cell suspension cultures","abstract":"Clomazone (2-(2-chlorophenyl)methyl-4,4-dimethyl-3-isoxazolidinone) is a selective preplant incorporated herbicide for use in soybean (Glycine max (L.) Merr.). Studies were conducted to determine the unknown mechanism of selectivity and site of action of clomazone in tolerant soybean (SB-M) and susceptible-cotton (Gossypium hirsutum (L.), COT-M) photomixotrophic cell suspension cultures.","abstract_html":"Clomazone (2-(2-chlorophenyl)methyl-4,4-dimethyl-3-isoxazolidinone) is a selective preplant incorporated herbicide for use in soybean (Glycine max (L.) Merr.). Studies were conducted to determine the unknown mechanism of selectivity and site of action of clomazone in tolerant soybean (SB-M) and susceptible-cotton (Gossypium hirsutum (L.), COT-M) photomixotrophic cell suspension cultures.","abstract_has_math":false,"creators":["Norman, Michael Alex"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Agronomy","degree_department":null,"school":null,"contributors":["Liebl, Rex A."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T12:03:02Z","date_published":"2011-05-07T12:03:02Z","updated_at":"2026-07-22T22:25:12Z","subjects":["Agriculture, Agronomy"],"languages":["eng"],"rights":["Copyright 1990 Norman, Michael Alex"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9026282","(UMI)AAI9026282"],"render_values":[{"text":"AAI9026282","href":null,"code":true},{"text":"(UMI)AAI9026282","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/19294","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Liebl, Rex A."]},{"key":"dc:creator","label":"Author","values":["Norman, Michael Alex"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T12:03:02Z","10000-01-01","1990"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Agronomy"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Agriculture, Agronomy"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1990 Norman, Michael Alex"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9026282","(UMI)AAI9026282","http://hdl.handle.net/2142/19294"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Clomazone (2-(2-chlorophenyl)methyl-4,4-dimethyl-3-isoxazolidinone) is a selective preplant incorporated herbicide for use in soybean (Glycine max (L.) Merr.). Studies were conducted to determine the unknown mechanism of selectivity and site of action of clomazone in tolerant soybean (SB-M) and susceptible-cotton (Gossypium hirsutum (L.), COT-M) photomixotrophic cell suspension cultures.","The mechanism of soybean tolerance to clomazone could not be attributed to differential absorption, translocation, and metabolism. SB-M cells absorbed more clomazone than COT-M cells and nearly identical levels of parental clomazone were recovered from both cell lines for all treatments. Assuming parental clomazone is the active form of the herbicide, these observations implicate differences at the site of action as the mechanism of selectivity. Although pooled metabolite fractions from both cell lines did not significantly reduce the leaf chlorophyll (chl) content of soybean seedlings, the pooled metabolite fraction from COT-M cells significantly reduced the leaf chl content of velvetleaf seedlings. Consequently, conversion of clomazone to an active form (bioactivation) by susceptible species may also account for soybean tolerance to clomazone.","Clomazone did not inhibit terpene synthesis in SB-M cells but significantly reduced the quantity and the level of MEV incorporation into chl, carotenoid, tocopherol, and plastoquinone of COT-M cells. Consequently, the site of clomazone action is after MEV and before GGPP within chloroplasts, the site of chl, carotenoid, tocopherol, and plastoquinone synthesis. Sequestration of clomazone from the chloroplast does not account for soybean tolerance to clomazone.","Made available in DSpace on 2011-05-07T12:03:02Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9026282.pdf: 2166034 bytes, checksum: f9c6ee154987021622fb3c4d687d7dcd (MD5) Previous issue date: 1990","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:35:58Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:14:24-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["The mechanism of selectivity and site of action of clomazone in tolerant-soybean and susceptible-cotton photomixotrophic cell suspension cultures"]}]}],"canonical_facts":{"dc:contributor":["Liebl, Rex A."],"dc:creator":["Norman, Michael Alex"],"dc:date":["2011-05-07T12:03:02Z","10000-01-01","1990"],"dc:description":["Clomazone (2-(2-chlorophenyl)methyl-4,4-dimethyl-3-isoxazolidinone) is a selective preplant incorporated herbicide for use in soybean (Glycine max (L.) Merr.). Studies were conducted to determine the unknown mechanism of selectivity and site of action of clomazone in tolerant soybean (SB-M) and susceptible-cotton (Gossypium hirsutum (L.), COT-M) photomixotrophic cell suspension cultures.","The mechanism of soybean tolerance to clomazone could not be attributed to differential absorption, translocation, and metabolism. SB-M cells absorbed more clomazone than COT-M cells and nearly identical levels of parental clomazone were recovered from both cell lines for all treatments. Assuming parental clomazone is the active form of the herbicide, these observations implicate differences at the site of action as the mechanism of selectivity. Although pooled metabolite fractions from both cell lines did not significantly reduce the leaf chlorophyll (chl) content of soybean seedlings, the pooled metabolite fraction from COT-M cells significantly reduced the leaf chl content of velvetleaf seedlings. Consequently, conversion of clomazone to an active form (bioactivation) by susceptible species may also account for soybean tolerance to clomazone.","Clomazone did not inhibit terpene synthesis in SB-M cells but significantly reduced the quantity and the level of MEV incorporation into chl, carotenoid, tocopherol, and plastoquinone of COT-M cells. Consequently, the site of clomazone action is after MEV and before GGPP within chloroplasts, the site of chl, carotenoid, tocopherol, and plastoquinone synthesis. Sequestration of clomazone from the chloroplast does not account for soybean tolerance to clomazone.","Made available in DSpace on 2011-05-07T12:03:02Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9026282.pdf: 2166034 bytes, checksum: f9c6ee154987021622fb3c4d687d7dcd (MD5) Previous issue date: 1990","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:35:58Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:14:24-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9026282","(UMI)AAI9026282","http://hdl.handle.net/2142/19294"],"dc:language":["eng"],"dc:rights":["Copyright 1990 Norman, Michael Alex"],"dc:subject":["Agriculture, Agronomy"],"dc:title":["The mechanism of selectivity and site of action of clomazone in tolerant-soybean and susceptible-cotton photomixotrophic cell suspension cultures"],"dc:type":["text"],"thesis:degree_discipline":["Agronomy"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:12Z"}