{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/19201"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/19201","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Polymerase chain reaction-based genotyping of swine MHC DQB and DRB alleles","abstract":"\"Molecular genotyping the swine major histocompatibility complex DQB and DRB loci using the PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) analysis technique is described. SLA DQB and DRB locus-specific oligonucleotide primers were designed to analyze expressed genes using the reverse transcription-polymerase chain reaction (RT-PCR) method. Five SLA DQB alleles and three SLA DRB alleles were sequenced after oligonucleotide-directed amplification of mRNA. Sequence alignment studies of SLA DQB and DRB $\\beta\\sb1$ domain sequences provided a similarity index among seven SLA DQB alleles of 87%, and the similarity index among five SLA DRB alleles was 85%; the similarity index among the SLA DQB and DRB alleles was 61%. Using this sequence information, SLA DQB and DRB oligonucleotide primers were designed for PCR genotyping the SLA DQB and DRB exon 2 ($\\beta\\sb1$) region from genomic DNA. These oligonucleotide primers were tested on genomic DNA from ten different swine breeds (Meishan, Yorkshire, Hampshire, Duroc, Landrace, Berkshire, Chester White, Spotted Poland China, Poland China and Swedish Landrace) to confirm their \"\"universal\"\" utility. DNA products after genomic PCR amplifications were subjected to genotyping by restriction enzyme digestion and subsequently electrophoresed on polyacrylamide gels. Several SLA DQB PCR-RFLP pattern types were observed using HaeIII (7 RFLPs) and RsaI (7 RFLPs): SLA DRB PCR-RFLP pattern types using MspI (3 RFLPs) and RsaI (5 RFLPs) were also demonstrated. Sixteen SLA DQB alleles and seven SLA DRB alleles were defined. Thus, locus-specific oligonucleotide primers provide a simple and rapid method for analyzing SLA DQB and DRB from genomic DNA isolated from small blood samples.\"","abstract_html":"&quot;Molecular genotyping the swine major histocompatibility complex DQB and DRB loci using the PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) analysis technique is described. SLA DQB and DRB locus-specific oligonucleotide primers were designed to analyze expressed genes using the reverse transcription-polymerase chain reaction (RT-PCR) method. Five SLA DQB alleles and three SLA DRB alleles were sequenced after oligonucleotide-directed amplification of mRNA. Sequence alignment studies of SLA DQB and DRB <span class=\"etd-inline-math\">&beta;\\sb1</span> domain sequences provided a similarity index among seven SLA DQB alleles of 87%, and the similarity index among five SLA DRB alleles was 85%; the similarity index among the SLA DQB and DRB alleles was 61%. Using this sequence information, SLA DQB and DRB oligonucleotide primers were designed for PCR genotyping the SLA DQB and DRB exon 2 (<span class=\"etd-inline-math\">&beta;\\sb1</span>) region from genomic DNA. These oligonucleotide primers were tested on genomic DNA from ten different swine breeds (Meishan, Yorkshire, Hampshire, Duroc, Landrace, Berkshire, Chester White, Spotted Poland China, Poland China and Swedish Landrace) to confirm their &quot;&quot;universal&quot;&quot; utility. DNA products after genomic PCR amplifications were subjected to genotyping by restriction enzyme digestion and subsequently electrophoresed on polyacrylamide gels. Several SLA DQB PCR-RFLP pattern types were observed using HaeIII (7 RFLPs) and RsaI (7 RFLPs): SLA DRB PCR-RFLP pattern types using MspI (3 RFLPs) and RsaI (5 RFLPs) were also demonstrated. Sixteen SLA DQB alleles and seven SLA DRB alleles were defined. Thus, locus-specific oligonucleotide primers provide a simple and rapid method for analyzing SLA DQB and DRB from genomic DNA isolated from small blood samples.&quot;","abstract_has_math":true,"creators":["Shia, Yun-Chao"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Animal Sciences","degree_department":null,"school":null,"contributors":["Schook, Lawrence B."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T11:59:59Z","date_published":"2011-05-07T11:59:59Z","updated_at":"2026-07-22T22:25:12Z","subjects":["Biology, Genetics","Agriculture, Animal Pathology"],"languages":["eng"],"rights":["Copyright 1993 Shia, Yun-Chao"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9412912","(UMI)AAI9412912"],"render_values":[{"text":"AAI9412912","href":null,"code":true},{"text":"(UMI)AAI9412912","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/19201","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Schook, Lawrence B."]},{"key":"dc:creator","label":"Author","values":["Shia, Yun-Chao"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T11:59:59Z","10000-01-01","1993"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Animal Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Genetics","Agriculture, Animal Pathology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1993 Shia, Yun-Chao"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9412912","(UMI)AAI9412912","http://hdl.handle.net/2142/19201"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["\"Molecular genotyping the swine major histocompatibility complex DQB and DRB loci using the PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) analysis technique is described. SLA DQB and DRB locus-specific oligonucleotide primers were designed to analyze expressed genes using the reverse transcription-polymerase chain reaction (RT-PCR) method. Five SLA DQB alleles and three SLA DRB alleles were sequenced after oligonucleotide-directed amplification of mRNA. Sequence alignment studies of SLA DQB and DRB $\\beta\\sb1$ domain sequences provided a similarity index among seven SLA DQB alleles of 87%, and the similarity index among five SLA DRB alleles was 85%; the similarity index among the SLA DQB and DRB alleles was 61%. Using this sequence information, SLA DQB and DRB oligonucleotide primers were designed for PCR genotyping the SLA DQB and DRB exon 2 ($\\beta\\sb1$) region from genomic DNA. These oligonucleotide primers were tested on genomic DNA from ten different swine breeds (Meishan, Yorkshire, Hampshire, Duroc, Landrace, Berkshire, Chester White, Spotted Poland China, Poland China and Swedish Landrace) to confirm their \"\"universal\"\" utility. DNA products after genomic PCR amplifications were subjected to genotyping by restriction enzyme digestion and subsequently electrophoresed on polyacrylamide gels. Several SLA DQB PCR-RFLP pattern types were observed using HaeIII (7 RFLPs) and RsaI (7 RFLPs): SLA DRB PCR-RFLP pattern types using MspI (3 RFLPs) and RsaI (5 RFLPs) were also demonstrated. Sixteen SLA DQB alleles and seven SLA DRB alleles were defined. Thus, locus-specific oligonucleotide primers provide a simple and rapid method for analyzing SLA DQB and DRB from genomic DNA isolated from small blood samples.\"","Made available in DSpace on 2011-05-07T11:59:59Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9412912.pdf: 4825596 bytes, checksum: d9fd595c4af759dba992db7f5d6dcb0f (MD5) Previous issue date: 1993","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:35:20Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:13:54-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Polymerase chain reaction-based genotyping of swine MHC DQB and DRB alleles"]}]}],"canonical_facts":{"dc:contributor":["Schook, Lawrence B."],"dc:creator":["Shia, Yun-Chao"],"dc:date":["2011-05-07T11:59:59Z","10000-01-01","1993"],"dc:description":["\"Molecular genotyping the swine major histocompatibility complex DQB and DRB loci using the PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) analysis technique is described. SLA DQB and DRB locus-specific oligonucleotide primers were designed to analyze expressed genes using the reverse transcription-polymerase chain reaction (RT-PCR) method. Five SLA DQB alleles and three SLA DRB alleles were sequenced after oligonucleotide-directed amplification of mRNA. Sequence alignment studies of SLA DQB and DRB $\\beta\\sb1$ domain sequences provided a similarity index among seven SLA DQB alleles of 87%, and the similarity index among five SLA DRB alleles was 85%; the similarity index among the SLA DQB and DRB alleles was 61%. Using this sequence information, SLA DQB and DRB oligonucleotide primers were designed for PCR genotyping the SLA DQB and DRB exon 2 ($\\beta\\sb1$) region from genomic DNA. These oligonucleotide primers were tested on genomic DNA from ten different swine breeds (Meishan, Yorkshire, Hampshire, Duroc, Landrace, Berkshire, Chester White, Spotted Poland China, Poland China and Swedish Landrace) to confirm their \"\"universal\"\" utility. DNA products after genomic PCR amplifications were subjected to genotyping by restriction enzyme digestion and subsequently electrophoresed on polyacrylamide gels. Several SLA DQB PCR-RFLP pattern types were observed using HaeIII (7 RFLPs) and RsaI (7 RFLPs): SLA DRB PCR-RFLP pattern types using MspI (3 RFLPs) and RsaI (5 RFLPs) were also demonstrated. Sixteen SLA DQB alleles and seven SLA DRB alleles were defined. Thus, locus-specific oligonucleotide primers provide a simple and rapid method for analyzing SLA DQB and DRB from genomic DNA isolated from small blood samples.\"","Made available in DSpace on 2011-05-07T11:59:59Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9412912.pdf: 4825596 bytes, checksum: d9fd595c4af759dba992db7f5d6dcb0f (MD5) Previous issue date: 1993","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:35:20Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:13:54-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9412912","(UMI)AAI9412912","http://hdl.handle.net/2142/19201"],"dc:language":["eng"],"dc:rights":["Copyright 1993 Shia, Yun-Chao"],"dc:subject":["Biology, Genetics","Agriculture, Animal Pathology"],"dc:title":["Polymerase chain reaction-based genotyping of swine MHC DQB and DRB alleles"],"dc:type":["text"],"thesis:degree_discipline":["Animal Sciences"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:12Z"}