{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/19167"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/19167","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Regulation of proto-oncogenes and immune system genes during CSF-1-induced macrophage differentiation","abstract":"CSF-1-induced bone marrow-derived macrophage (BMDM) differentiating in vitro acquire both antigen presenting and tumoricidal capabilities. In order to understand the molecular origin of macrophage diversity transcriptional and post-transcriptional regulation of genes involved in affector and effector functions were studied. Transcripts of c-fms and c-fos were detected at all stages of differentiation, whereas c-myc gene expression was highest during the proliferative stages of development. Endotoxin treatment of BMDM enhanced the expression of all proto-oncogenes, however the c-myc mRNA levels were highest on day 3 of culture. IFN-$\\gamma$ treatment of BMDM cultures also enhanced the transcription of proto-oncogenes. Thus, it is felt that c-fms, c-fos and c-myc may be related to the complex mechanism of macrophage activation as evidenced by the stage-specific gene regulation. Interesting observations were made on the study of genes involved with functional state macrophage. Transcription of MHC class I and II genes occurred in the absence of any known Ia-inducing factor and the transcripts reached a maximum (3- to 4-fold) between days 5-7 of culture. IFN-$\\gamma$ enhanced the transcription of both class I (2- to 5-fold) and II (2- to 10-fold) genes. Nuclear run-off assay results demonstrated that endotoxin treatment of the BMDM cultures augmented expression of both class I (2- to 3-fold) and II (2- to 3-fold) genes suggesting a post-transcriptional control of the MHC genes in the absence of an Ia-inducing factor. Upon endotoxin stimulation, transcription of IL-1$\\alpha$ and IL-1$\\beta$ showed almost similar kinetics which paralleled the kinetics of accumulation of steady state mRNA. This suggested that the expression of IL-1 genes are regulated transcriptionally. Tough both IFN-$\\gamma$ and endotoxin enhanced (4- to 5-fold) the transcription of TNF-$\\alpha$ gene, endotoxin had a more pronounced effect. The kinetics of TNF-$\\alpha$ transcription paralleled the kinetics of steady state TNF-$\\alpha$ mRNA accumulation, thereby suggesting both transcriptional and post-transcriptional control in the expression of TNF-$\\alpha$ gene. Thus, these findings indicate that during macrophage development there is a sequential expression of immune system genes which is intrinsically determined.","abstract_html":"CSF-1-induced bone marrow-derived macrophage (BMDM) differentiating in vitro acquire both antigen presenting and tumoricidal capabilities. In order to understand the molecular origin of macrophage diversity transcriptional and post-transcriptional regulation of genes involved in affector and effector functions were studied. Transcripts of c-fms and c-fos were detected at all stages of differentiation, whereas c-myc gene expression was highest during the proliferative stages of development. Endotoxin treatment of BMDM enhanced the expression of all proto-oncogenes, however the c-myc mRNA levels were highest on day 3 of culture. IFN-<span class=\"etd-inline-math\">&gamma;</span> treatment of BMDM cultures also enhanced the transcription of proto-oncogenes. Thus, it is felt that c-fms, c-fos and c-myc may be related to the complex mechanism of macrophage activation as evidenced by the stage-specific gene regulation. Interesting observations were made on the study of genes involved with functional state macrophage. Transcription of MHC class I and II genes occurred in the absence of any known Ia-inducing factor and the transcripts reached a maximum (3- to 4-fold) between days 5-7 of culture. IFN-<span class=\"etd-inline-math\">&gamma;</span> enhanced the transcription of both class I (2- to 5-fold) and II (2- to 10-fold) genes. Nuclear run-off assay results demonstrated that endotoxin treatment of the BMDM cultures augmented expression of both class I (2- to 3-fold) and II (2- to 3-fold) genes suggesting a post-transcriptional control of the MHC genes in the absence of an Ia-inducing factor. Upon endotoxin stimulation, transcription of IL-1<span class=\"etd-inline-math\">&alpha;</span> and IL-1<span class=\"etd-inline-math\">&beta;</span> showed almost similar kinetics which paralleled the kinetics of accumulation of steady state mRNA. This suggested that the expression of IL-1 genes are regulated transcriptionally. Tough both IFN-<span class=\"etd-inline-math\">&gamma;</span> and endotoxin enhanced (4- to 5-fold) the transcription of TNF-<span class=\"etd-inline-math\">&alpha;</span> gene, endotoxin had a more pronounced effect. The kinetics of TNF-<span class=\"etd-inline-math\">&alpha;</span> transcription paralleled the kinetics of steady state TNF-<span class=\"etd-inline-math\">&alpha;</span> mRNA accumulation, thereby suggesting both transcriptional and post-transcriptional control in the expression of TNF-<span class=\"etd-inline-math\">&alpha;</span> gene. Thus, these findings indicate that during macrophage development there is a sequential expression of immune system genes which is intrinsically determined.","abstract_has_math":true,"creators":["Ghildyal, Namit"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Animal Science","degree_department":null,"school":null,"contributors":["Schook, Lawrence B."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T11:59:00Z","date_published":"2011-05-07T11:59:00Z","updated_at":"2026-07-22T22:25:12Z","subjects":["Biology, Molecular","Health Sciences, Immunology"],"languages":["eng"],"rights":["Copyright 1990 Ghildyal, Namit"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9021684","(UMI)AAI9021684"],"render_values":[{"text":"AAI9021684","href":null,"code":true},{"text":"(UMI)AAI9021684","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/19167","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Schook, Lawrence B."]},{"key":"dc:creator","label":"Author","values":["Ghildyal, Namit"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T11:59:00Z","10000-01-01","1990"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Animal Science"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Molecular","Health Sciences, Immunology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1990 Ghildyal, Namit"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9021684","(UMI)AAI9021684","http://hdl.handle.net/2142/19167"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["CSF-1-induced bone marrow-derived macrophage (BMDM) differentiating in vitro acquire both antigen presenting and tumoricidal capabilities. In order to understand the molecular origin of macrophage diversity transcriptional and post-transcriptional regulation of genes involved in affector and effector functions were studied. Transcripts of c-fms and c-fos were detected at all stages of differentiation, whereas c-myc gene expression was highest during the proliferative stages of development. Endotoxin treatment of BMDM enhanced the expression of all proto-oncogenes, however the c-myc mRNA levels were highest on day 3 of culture. IFN-$\\gamma$ treatment of BMDM cultures also enhanced the transcription of proto-oncogenes. Thus, it is felt that c-fms, c-fos and c-myc may be related to the complex mechanism of macrophage activation as evidenced by the stage-specific gene regulation. Interesting observations were made on the study of genes involved with functional state macrophage. Transcription of MHC class I and II genes occurred in the absence of any known Ia-inducing factor and the transcripts reached a maximum (3- to 4-fold) between days 5-7 of culture. IFN-$\\gamma$ enhanced the transcription of both class I (2- to 5-fold) and II (2- to 10-fold) genes. Nuclear run-off assay results demonstrated that endotoxin treatment of the BMDM cultures augmented expression of both class I (2- to 3-fold) and II (2- to 3-fold) genes suggesting a post-transcriptional control of the MHC genes in the absence of an Ia-inducing factor. Upon endotoxin stimulation, transcription of IL-1$\\alpha$ and IL-1$\\beta$ showed almost similar kinetics which paralleled the kinetics of accumulation of steady state mRNA. This suggested that the expression of IL-1 genes are regulated transcriptionally. Tough both IFN-$\\gamma$ and endotoxin enhanced (4- to 5-fold) the transcription of TNF-$\\alpha$ gene, endotoxin had a more pronounced effect. The kinetics of TNF-$\\alpha$ transcription paralleled the kinetics of steady state TNF-$\\alpha$ mRNA accumulation, thereby suggesting both transcriptional and post-transcriptional control in the expression of TNF-$\\alpha$ gene. Thus, these findings indicate that during macrophage development there is a sequential expression of immune system genes which is intrinsically determined.","Made available in DSpace on 2011-05-07T11:59:00Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9021684.pdf: 4965694 bytes, checksum: cd499378cf5205d9b7d3fbf6a3143641 (MD5) Previous issue date: 1990","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:35:07Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:13:41-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Regulation of proto-oncogenes and immune system genes during CSF-1-induced macrophage differentiation"]}]}],"canonical_facts":{"dc:contributor":["Schook, Lawrence B."],"dc:creator":["Ghildyal, Namit"],"dc:date":["2011-05-07T11:59:00Z","10000-01-01","1990"],"dc:description":["CSF-1-induced bone marrow-derived macrophage (BMDM) differentiating in vitro acquire both antigen presenting and tumoricidal capabilities. In order to understand the molecular origin of macrophage diversity transcriptional and post-transcriptional regulation of genes involved in affector and effector functions were studied. Transcripts of c-fms and c-fos were detected at all stages of differentiation, whereas c-myc gene expression was highest during the proliferative stages of development. Endotoxin treatment of BMDM enhanced the expression of all proto-oncogenes, however the c-myc mRNA levels were highest on day 3 of culture. IFN-$\\gamma$ treatment of BMDM cultures also enhanced the transcription of proto-oncogenes. Thus, it is felt that c-fms, c-fos and c-myc may be related to the complex mechanism of macrophage activation as evidenced by the stage-specific gene regulation. Interesting observations were made on the study of genes involved with functional state macrophage. Transcription of MHC class I and II genes occurred in the absence of any known Ia-inducing factor and the transcripts reached a maximum (3- to 4-fold) between days 5-7 of culture. IFN-$\\gamma$ enhanced the transcription of both class I (2- to 5-fold) and II (2- to 10-fold) genes. Nuclear run-off assay results demonstrated that endotoxin treatment of the BMDM cultures augmented expression of both class I (2- to 3-fold) and II (2- to 3-fold) genes suggesting a post-transcriptional control of the MHC genes in the absence of an Ia-inducing factor. Upon endotoxin stimulation, transcription of IL-1$\\alpha$ and IL-1$\\beta$ showed almost similar kinetics which paralleled the kinetics of accumulation of steady state mRNA. This suggested that the expression of IL-1 genes are regulated transcriptionally. Tough both IFN-$\\gamma$ and endotoxin enhanced (4- to 5-fold) the transcription of TNF-$\\alpha$ gene, endotoxin had a more pronounced effect. The kinetics of TNF-$\\alpha$ transcription paralleled the kinetics of steady state TNF-$\\alpha$ mRNA accumulation, thereby suggesting both transcriptional and post-transcriptional control in the expression of TNF-$\\alpha$ gene. Thus, these findings indicate that during macrophage development there is a sequential expression of immune system genes which is intrinsically determined.","Made available in DSpace on 2011-05-07T11:59:00Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9021684.pdf: 4965694 bytes, checksum: cd499378cf5205d9b7d3fbf6a3143641 (MD5) Previous issue date: 1990","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:35:07Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:13:41-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9021684","(UMI)AAI9021684","http://hdl.handle.net/2142/19167"],"dc:language":["eng"],"dc:rights":["Copyright 1990 Ghildyal, Namit"],"dc:subject":["Biology, Molecular","Health Sciences, Immunology"],"dc:title":["Regulation of proto-oncogenes and immune system genes during CSF-1-induced macrophage differentiation"],"dc:type":["text"],"thesis:degree_discipline":["Animal Science"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:12Z"}