{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/19164"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/19164","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Identification and characterization of aquatic soluble unreactive phosphorus in the hydrosphere with 31-phosphorus Fourier transform nuclear magnetic resonance spectroscopy and high-performance liquid chromatography","abstract":"The identity and characteristics of aquatic soluble phosphorus (SUP) present in a small mid-western mesotropic lake were examined with 31-Phosphorus Fourier Transform Nuclear Magnetic Resonance Spectroscopy $\\rm (\\sp{31}P$ FT-NMR) and High Performance Liquid Chromatography (HPLC). Samples were concentrated and fractionated according to molecular size using a series of ultrafiltration (UF) and reverse osmosis (RO) membranes. The UF membrane pore sizes were 30,000 Da and 1,000 Da while the RO membrane had a 95% NaCl rejection rating. The SUP was analyzed as a function of apparent molecular size and season. In the 30,000 Da retentate, monoester phosphates, diester phosphates, and phophonates were detected. In the 1,000 Da retentate, monoester and diester phosphates were seen, and in the reverse osmosis retentate, orthophosphate and monoester phosphates were detected. Seasonal changes in the monoester phosphates of the 1,000 Da retentate were detected with $\\rm\\sp{31}P$ FT-NMR and HPLC. Enhancement of the $\\rm\\sp{31}P$ FT-NMR spectral resolution and sample characterization was achieved with the use of $\\rm T\\sb1$ relaxation agents and aqueous soluble lanthanide shift reagents. A Mississippi River sample, fractionated into a 1,000K Da retentate and a RO retentate sample, was examined with $\\rm\\sp{31}P$ FT-NMR and HPLC. The SUP in the 1,000 Da retentate sample was found to be different from the lake samples in that it contained polyphosphates and possibly phosphonates, while the RO retentate was very similar to the lake RO retentate sample. This research has also demonstrated that changes occur with the SUP in the sample during concentration by UF and RO methods. It appears the phosphorus compounds are becoming incorporated into an aggregate structure which forms during the concentration procedure. Possible identities of this aggregate structure were examined using a variety of extraction and degradation methods. Extraction of SUP with ion pairing reagents and hydrophobic resins was started to be examined as a method for further enhancing SUP characterization ability by obtaining samples free from $\\rm\\sp{31}P$ FT-NMR and HPLC interferents.","abstract_html":"The identity and characteristics of aquatic soluble phosphorus (SUP) present in a small mid-western mesotropic lake were examined with 31-Phosphorus Fourier Transform Nuclear Magnetic Resonance Spectroscopy $\\rm (\\sp{31}P$ FT-NMR) and High Performance Liquid Chromatography (HPLC). Samples were concentrated and fractionated according to molecular size using a series of ultrafiltration (UF) and reverse osmosis (RO) membranes. The UF membrane pore sizes were 30,000 Da and 1,000 Da while the RO membrane had a 95% NaCl rejection rating. The SUP was analyzed as a function of apparent molecular size and season. In the 30,000 Da retentate, monoester phosphates, diester phosphates, and phophonates were detected. In the 1,000 Da retentate, monoester and diester phosphates were seen, and in the reverse osmosis retentate, orthophosphate and monoester phosphates were detected. Seasonal changes in the monoester phosphates of the 1,000 Da retentate were detected with $\\rm\\sp{31}P$ FT-NMR and HPLC. Enhancement of the $\\rm\\sp{31}P$ FT-NMR spectral resolution and sample characterization was achieved with the use of $\\rm T\\sb1$ relaxation agents and aqueous soluble lanthanide shift reagents. A Mississippi River sample, fractionated into a 1,000K Da retentate and a RO retentate sample, was examined with $\\rm\\sp{31}P$ FT-NMR and HPLC. The SUP in the 1,000 Da retentate sample was found to be different from the lake samples in that it contained polyphosphates and possibly phosphonates, while the RO retentate was very similar to the lake RO retentate sample. This research has also demonstrated that changes occur with the SUP in the sample during concentration by UF and RO methods. It appears the phosphorus compounds are becoming incorporated into an aggregate structure which forms during the concentration procedure. Possible identities of this aggregate structure were examined using a variety of extraction and degradation methods. Extraction of SUP with ion pairing reagents and hydrophobic resins was started to be examined as a method for further enhancing SUP characterization ability by obtaining samples free from $\\rm\\sp{31}P$ FT-NMR and HPLC interferents.","abstract_has_math":true,"creators":["Nanny, Mark Allen"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Environmental Engineering","degree_department":null,"school":null,"contributors":["Minear, Roger A."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T11:58:54Z","date_published":"2011-05-07T11:58:54Z","updated_at":"2026-07-22T22:25:12Z","subjects":["Biogeochemistry","Environmental Sciences","Biology, Limnology"],"languages":["eng"],"rights":["Copyright 1994 Nanny, Mark Allen"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9512497","(UMI)AAI9512497"],"render_values":[{"text":"AAI9512497","href":null,"code":true},{"text":"(UMI)AAI9512497","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/19164","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Minear, Roger A."]},{"key":"dc:creator","label":"Author","values":["Nanny, Mark Allen"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T11:58:54Z","10000-01-01","1994"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Environmental Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biogeochemistry","Environmental Sciences","Biology, Limnology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1994 Nanny, Mark Allen"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9512497","(UMI)AAI9512497","http://hdl.handle.net/2142/19164"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The identity and characteristics of aquatic soluble phosphorus (SUP) present in a small mid-western mesotropic lake were examined with 31-Phosphorus Fourier Transform Nuclear Magnetic Resonance Spectroscopy $\\rm (\\sp{31}P$ FT-NMR) and High Performance Liquid Chromatography (HPLC). Samples were concentrated and fractionated according to molecular size using a series of ultrafiltration (UF) and reverse osmosis (RO) membranes. The UF membrane pore sizes were 30,000 Da and 1,000 Da while the RO membrane had a 95% NaCl rejection rating. The SUP was analyzed as a function of apparent molecular size and season. In the 30,000 Da retentate, monoester phosphates, diester phosphates, and phophonates were detected. In the 1,000 Da retentate, monoester and diester phosphates were seen, and in the reverse osmosis retentate, orthophosphate and monoester phosphates were detected. Seasonal changes in the monoester phosphates of the 1,000 Da retentate were detected with $\\rm\\sp{31}P$ FT-NMR and HPLC. Enhancement of the $\\rm\\sp{31}P$ FT-NMR spectral resolution and sample characterization was achieved with the use of $\\rm T\\sb1$ relaxation agents and aqueous soluble lanthanide shift reagents. A Mississippi River sample, fractionated into a 1,000K Da retentate and a RO retentate sample, was examined with $\\rm\\sp{31}P$ FT-NMR and HPLC. The SUP in the 1,000 Da retentate sample was found to be different from the lake samples in that it contained polyphosphates and possibly phosphonates, while the RO retentate was very similar to the lake RO retentate sample. This research has also demonstrated that changes occur with the SUP in the sample during concentration by UF and RO methods. It appears the phosphorus compounds are becoming incorporated into an aggregate structure which forms during the concentration procedure. Possible identities of this aggregate structure were examined using a variety of extraction and degradation methods. Extraction of SUP with ion pairing reagents and hydrophobic resins was started to be examined as a method for further enhancing SUP characterization ability by obtaining samples free from $\\rm\\sp{31}P$ FT-NMR and HPLC interferents.","Made available in DSpace on 2011-05-07T11:58:54Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9512497.pdf: 9781140 bytes, checksum: e9786bcc69469bd1874a2e0cf2ef61b6 (MD5) Previous issue date: 1994","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:35:05Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:13:41-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"]},{"key":"dc:title","label":"Title","values":["Identification and characterization of aquatic soluble unreactive phosphorus in the hydrosphere with 31-phosphorus Fourier transform nuclear magnetic resonance spectroscopy and high-performance liquid chromatography"]}]}],"canonical_facts":{"dc:contributor":["Minear, Roger A."],"dc:creator":["Nanny, Mark Allen"],"dc:date":["2011-05-07T11:58:54Z","10000-01-01","1994"],"dc:description":["The identity and characteristics of aquatic soluble phosphorus (SUP) present in a small mid-western mesotropic lake were examined with 31-Phosphorus Fourier Transform Nuclear Magnetic Resonance Spectroscopy $\\rm (\\sp{31}P$ FT-NMR) and High Performance Liquid Chromatography (HPLC). Samples were concentrated and fractionated according to molecular size using a series of ultrafiltration (UF) and reverse osmosis (RO) membranes. The UF membrane pore sizes were 30,000 Da and 1,000 Da while the RO membrane had a 95% NaCl rejection rating. The SUP was analyzed as a function of apparent molecular size and season. In the 30,000 Da retentate, monoester phosphates, diester phosphates, and phophonates were detected. In the 1,000 Da retentate, monoester and diester phosphates were seen, and in the reverse osmosis retentate, orthophosphate and monoester phosphates were detected. Seasonal changes in the monoester phosphates of the 1,000 Da retentate were detected with $\\rm\\sp{31}P$ FT-NMR and HPLC. Enhancement of the $\\rm\\sp{31}P$ FT-NMR spectral resolution and sample characterization was achieved with the use of $\\rm T\\sb1$ relaxation agents and aqueous soluble lanthanide shift reagents. A Mississippi River sample, fractionated into a 1,000K Da retentate and a RO retentate sample, was examined with $\\rm\\sp{31}P$ FT-NMR and HPLC. The SUP in the 1,000 Da retentate sample was found to be different from the lake samples in that it contained polyphosphates and possibly phosphonates, while the RO retentate was very similar to the lake RO retentate sample. This research has also demonstrated that changes occur with the SUP in the sample during concentration by UF and RO methods. It appears the phosphorus compounds are becoming incorporated into an aggregate structure which forms during the concentration procedure. Possible identities of this aggregate structure were examined using a variety of extraction and degradation methods. Extraction of SUP with ion pairing reagents and hydrophobic resins was started to be examined as a method for further enhancing SUP characterization ability by obtaining samples free from $\\rm\\sp{31}P$ FT-NMR and HPLC interferents.","Made available in DSpace on 2011-05-07T11:58:54Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9512497.pdf: 9781140 bytes, checksum: e9786bcc69469bd1874a2e0cf2ef61b6 (MD5) Previous issue date: 1994","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:35:05Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:13:41-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission","U of I Only"],"dc:identifier":["AAI9512497","(UMI)AAI9512497","http://hdl.handle.net/2142/19164"],"dc:language":["eng"],"dc:rights":["Copyright 1994 Nanny, Mark Allen"],"dc:subject":["Biogeochemistry","Environmental Sciences","Biology, Limnology"],"dc:title":["Identification and characterization of aquatic soluble unreactive phosphorus in the hydrosphere with 31-phosphorus Fourier transform nuclear magnetic resonance spectroscopy and high-performance liquid chromatography"],"dc:type":["text"],"thesis:degree_discipline":["Environmental Engineering"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:12Z"}