{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/19090"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/19090","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Effects of selenite on metabolic events during the proliferation of canine mammary tumor cells","abstract":"Increased cytoplasmic and nuclear selenium retention in CMT-13 cells was correlated with cell growth inhibition caused by selenite supplementation. Greater quantities of cytosolic selenium-containing proteins and a nuclear selenium-containing protein were detected as the quantity of selenium within CMT-13 cells increased. One of the antiproliferation effects of selenite on CMT-13 cells is accompanied by decreased rates of macromolecule synthesis, increased cellular macromolecule contents and increased cell size and multiple nuclei, indicating that enhanced cell fusion occurred. Selenite modulated the S and M phases during the cell cycle. Selenite increased the activity of the 114 KD protein kinase only during the S phase. Selenite treatment during the M phase resulted in a disappearance of a 53 KD kinase and the appearance of a 47 KD kinase. The cellular $\\rm\\sp{32}P$ incorporation into macromolecules increased when inhibition of cell growth was observed by supplementation of selenite. Selenite generally increased the phosphorylation of nuclear phosphoproteins in the S phase, especially the phosphorylation of a 46 KD protein. Selenite inhibited hyperphosphorylation of the 21, 62 and 108 KD proteins during the M phase. Results of this study suggest that perturbations in protein phosphorylation may explain the ability of selenite to alter cell proliferation.","abstract_html":"Increased cytoplasmic and nuclear selenium retention in CMT-13 cells was correlated with cell growth inhibition caused by selenite supplementation. Greater quantities of cytosolic selenium-containing proteins and a nuclear selenium-containing protein were detected as the quantity of selenium within CMT-13 cells increased. One of the antiproliferation effects of selenite on CMT-13 cells is accompanied by decreased rates of macromolecule synthesis, increased cellular macromolecule contents and increased cell size and multiple nuclei, indicating that enhanced cell fusion occurred. Selenite modulated the S and M phases during the cell cycle. Selenite increased the activity of the 114 KD protein kinase only during the S phase. Selenite treatment during the M phase resulted in a disappearance of a 53 KD kinase and the appearance of a 47 KD kinase. The cellular $\\rm\\sp{32}P$ incorporation into macromolecules increased when inhibition of cell growth was observed by supplementation of selenite. Selenite generally increased the phosphorylation of nuclear phosphoproteins in the S phase, especially the phosphorylation of a 46 KD protein. Selenite inhibited hyperphosphorylation of the 21, 62 and 108 KD proteins during the M phase. Results of this study suggest that perturbations in protein phosphorylation may explain the ability of selenite to alter cell proliferation.","abstract_has_math":true,"creators":["Hwang, Kyung Hee"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Nutritional Sciences","degree_department":null,"school":null,"contributors":["Milner, J.A."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1994,"date_issued":"1994","date_published":"1994","updated_at":"2026-07-22T22:25:12Z","subjects":["Biology, Molecular","Biology, Cell","Agriculture, Animal Culture and Nutrition"],"languages":["eng"],"rights":["Copyright 1994 Hwang, Kyung Hee"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9503220","(UMI)AAI9503220"],"render_values":[{"text":"AAI9503220","href":null,"code":true},{"text":"(UMI)AAI9503220","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/19090","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Milner, J.A."]},{"key":"dc:creator","label":"Author","values":["Hwang, Kyung Hee"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["1994","2011-05-07T11:56:38Z","10000-01-01"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Nutritional Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Molecular","Biology, Cell","Agriculture, Animal Culture and Nutrition"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1994 Hwang, Kyung Hee"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9503220","(UMI)AAI9503220","http://hdl.handle.net/2142/19090"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Increased cytoplasmic and nuclear selenium retention in CMT-13 cells was correlated with cell growth inhibition caused by selenite supplementation. Greater quantities of cytosolic selenium-containing proteins and a nuclear selenium-containing protein were detected as the quantity of selenium within CMT-13 cells increased. One of the antiproliferation effects of selenite on CMT-13 cells is accompanied by decreased rates of macromolecule synthesis, increased cellular macromolecule contents and increased cell size and multiple nuclei, indicating that enhanced cell fusion occurred. Selenite modulated the S and M phases during the cell cycle. Selenite increased the activity of the 114 KD protein kinase only during the S phase. Selenite treatment during the M phase resulted in a disappearance of a 53 KD kinase and the appearance of a 47 KD kinase. The cellular $\\rm\\sp{32}P$ incorporation into macromolecules increased when inhibition of cell growth was observed by supplementation of selenite. Selenite generally increased the phosphorylation of nuclear phosphoproteins in the S phase, especially the phosphorylation of a 46 KD protein. Selenite inhibited hyperphosphorylation of the 21, 62 and 108 KD proteins during the M phase. Results of this study suggest that perturbations in protein phosphorylation may explain the ability of selenite to alter cell proliferation.","Made available in DSpace on 2011-05-07T11:56:38Z (GMT). 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Greater quantities of cytosolic selenium-containing proteins and a nuclear selenium-containing protein were detected as the quantity of selenium within CMT-13 cells increased. One of the antiproliferation effects of selenite on CMT-13 cells is accompanied by decreased rates of macromolecule synthesis, increased cellular macromolecule contents and increased cell size and multiple nuclei, indicating that enhanced cell fusion occurred. Selenite modulated the S and M phases during the cell cycle. Selenite increased the activity of the 114 KD protein kinase only during the S phase. Selenite treatment during the M phase resulted in a disappearance of a 53 KD kinase and the appearance of a 47 KD kinase. The cellular $\\rm\\sp{32}P$ incorporation into macromolecules increased when inhibition of cell growth was observed by supplementation of selenite. Selenite generally increased the phosphorylation of nuclear phosphoproteins in the S phase, especially the phosphorylation of a 46 KD protein. Selenite inhibited hyperphosphorylation of the 21, 62 and 108 KD proteins during the M phase. Results of this study suggest that perturbations in protein phosphorylation may explain the ability of selenite to alter cell proliferation.","Made available in DSpace on 2011-05-07T11:56:38Z (GMT). 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