{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/18961"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/18961","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Placental separation in cattle: Arachidonic acid metabolites, steroids and leukocyte function","abstract":"U of I Only","abstract_html":"U of I Only","abstract_has_math":false,"creators":["Hoedemaker, Martina"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Veterinary Medical Science","degree_department":null,"school":null,"contributors":["Wagner, William C."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-07T11:52:43Z","date_published":"2011-05-07T11:52:43Z","updated_at":"2026-07-22T22:25:12Z","subjects":["Agriculture, Animal Pathology","Biology, Veterinary Science"],"languages":["eng"],"rights":["Copyright 1990 Hoedemaker, Martina"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9114265","(UMI)AAI9114265"],"render_values":[{"text":"AAI9114265","href":null,"code":true},{"text":"(UMI)AAI9114265","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/18961","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Wagner, William C."]},{"key":"dc:creator","label":"Author","values":["Hoedemaker, Martina"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-05-07T11:52:43Z","10000-01-01","1990"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Veterinary Medical Science"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Agriculture, Animal Pathology","Biology, Veterinary Science"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 1990 Hoedemaker, Martina"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["AAI9114265","(UMI)AAI9114265","http://hdl.handle.net/2142/18961"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["U of I Only","In the following investigation, in vivo and in vitro arachidonic acid (AA) and steroid metabolism as well as function of circulating polymorphonuclear neutrophil leukocytes (PMN) during the last month of pregnancy in cows, and possible interrelationships between those three parameters, were studied. Concentrations of 6-keto-prostaglandin F$\\sb{1\\alpha}$ (6-keto-PGF$\\sb{1\\alpha}$), prostaglandin F$\\sb{2\\alpha}$, (PGF$\\sb{2\\alpha}$) and prostaglandin E$\\sb2$ (PGE$\\sb2$) in carotid artery blood increased throughout the last month of pregnancy. Concentrations of 6-keto-PGF$\\sb{1\\alpha}$ and PGE$\\sb2$ were higher in uterine vein than in carotid artery blood, a fact which was also observed as a trend for PGF$\\sb{2\\alpha}$, thromboxane B$\\sb2$ and 15-hydroxyeicosatetraenoic acid (15-HETE). Metabolism of AA by fetal placental tissue in vitro was markedly increased as pregnancy progressed. The major metabolites synthesized were: 13,14-dihydro-15-keto-PGE$\\sb2$, PGE$\\sb2$, 12-hydroxy-heptadecatrienoic acid and 15-HETE. Fetal placental tissue in vitro also produced estrogens in increasing amounts throughout the experimental period. The application of 5 mg dexamethasone to the dam on Day 255 of gestation did not affect placental estrogen synthesis in vitro. In contrast, cortisol applied in vitro stimulated estrogen synthesis in a dose-dependent manner, probably by stimulating enzymes involved in the $\\Delta$5 steroidogenic pathway which turned out to be the major placental estrogenic pathway in cows. Iodination of proteins and cytochrome C reduction by PMN, both measures of the oxygen-dependent antimicrobial system, were decreased at parturition, whereas ingestion capacity was stimulated at parturition. Fetal placental tissue and uterine wall tissue extracts attracted PMN, whereas skeletal muscle extracts were without effect. Five eicosanoids (PGF$\\sb{2\\alpha}$, leukotriene B$\\sb4$, 5- and 15-HETE, and lipoxin B$\\sb4$), and estrogens were chemoattractant. Prostacyclin inhibited iodination of proteins, whereas PGF$\\sb{2\\alpha}$ stimulated ingestion capacity of PMN. Conditioned media from cultured fetal placental tissue attracted PMN, stimulated chemotaxis, and inhibited iodination of proteins, cytochrome C reduction and antibody-dependent cell-mediated cytotoxicity. It is concluded that substances produced within the uterine compartment (prostacyclin, PGF$\\sb{2\\alpha}$) and by the placenta (15-HETE, estrogens) might be associated with the chemoattractant properties of fetal placental tissue and uterine tissues, and the changes in PMN function observed at the end of pregnancy. The latter might render the animal more susceptible to bacterial infections in the periparturient period.","Made available in DSpace on 2011-05-07T11:52:43Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9114265.pdf: 10169161 bytes, checksum: 539945b65d19c4df18f4a0dc440036a7 (MD5) Previous issue date: 1990","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:33:42Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:12:32-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission"]},{"key":"dc:title","label":"Title","values":["Placental separation in cattle: Arachidonic acid metabolites, steroids and leukocyte function"]}]}],"canonical_facts":{"dc:contributor":["Wagner, William C."],"dc:creator":["Hoedemaker, Martina"],"dc:date":["2011-05-07T11:52:43Z","10000-01-01","1990"],"dc:description":["U of I Only","In the following investigation, in vivo and in vitro arachidonic acid (AA) and steroid metabolism as well as function of circulating polymorphonuclear neutrophil leukocytes (PMN) during the last month of pregnancy in cows, and possible interrelationships between those three parameters, were studied. Concentrations of 6-keto-prostaglandin F$\\sb{1\\alpha}$ (6-keto-PGF$\\sb{1\\alpha}$), prostaglandin F$\\sb{2\\alpha}$, (PGF$\\sb{2\\alpha}$) and prostaglandin E$\\sb2$ (PGE$\\sb2$) in carotid artery blood increased throughout the last month of pregnancy. Concentrations of 6-keto-PGF$\\sb{1\\alpha}$ and PGE$\\sb2$ were higher in uterine vein than in carotid artery blood, a fact which was also observed as a trend for PGF$\\sb{2\\alpha}$, thromboxane B$\\sb2$ and 15-hydroxyeicosatetraenoic acid (15-HETE). Metabolism of AA by fetal placental tissue in vitro was markedly increased as pregnancy progressed. The major metabolites synthesized were: 13,14-dihydro-15-keto-PGE$\\sb2$, PGE$\\sb2$, 12-hydroxy-heptadecatrienoic acid and 15-HETE. Fetal placental tissue in vitro also produced estrogens in increasing amounts throughout the experimental period. The application of 5 mg dexamethasone to the dam on Day 255 of gestation did not affect placental estrogen synthesis in vitro. In contrast, cortisol applied in vitro stimulated estrogen synthesis in a dose-dependent manner, probably by stimulating enzymes involved in the $\\Delta$5 steroidogenic pathway which turned out to be the major placental estrogenic pathway in cows. Iodination of proteins and cytochrome C reduction by PMN, both measures of the oxygen-dependent antimicrobial system, were decreased at parturition, whereas ingestion capacity was stimulated at parturition. Fetal placental tissue and uterine wall tissue extracts attracted PMN, whereas skeletal muscle extracts were without effect. Five eicosanoids (PGF$\\sb{2\\alpha}$, leukotriene B$\\sb4$, 5- and 15-HETE, and lipoxin B$\\sb4$), and estrogens were chemoattractant. Prostacyclin inhibited iodination of proteins, whereas PGF$\\sb{2\\alpha}$ stimulated ingestion capacity of PMN. Conditioned media from cultured fetal placental tissue attracted PMN, stimulated chemotaxis, and inhibited iodination of proteins, cytochrome C reduction and antibody-dependent cell-mediated cytotoxicity. It is concluded that substances produced within the uterine compartment (prostacyclin, PGF$\\sb{2\\alpha}$) and by the placenta (15-HETE, estrogens) might be associated with the chemoattractant properties of fetal placental tissue and uterine tissues, and the changes in PMN function observed at the end of pregnancy. The latter might render the animal more susceptible to bacterial infections in the periparturient period.","Made available in DSpace on 2011-05-07T11:52:43Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9114265.pdf: 10169161 bytes, checksum: 539945b65d19c4df18f4a0dc440036a7 (MD5) Previous issue date: 1990","Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:33:42Z Item is restricted indefinitely.","Restriction data tranferred 2014-07-01T11:12:32-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission","ETDs are only available to UIUC Users without author permission"],"dc:identifier":["AAI9114265","(UMI)AAI9114265","http://hdl.handle.net/2142/18961"],"dc:language":["eng"],"dc:rights":["Copyright 1990 Hoedemaker, Martina"],"dc:subject":["Agriculture, Animal Pathology","Biology, Veterinary Science"],"dc:title":["Placental separation in cattle: Arachidonic acid metabolites, steroids and leukocyte function"],"dc:type":["text"],"thesis:degree_discipline":["Veterinary Medical Science"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:12Z"}