{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/17064"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/17064","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Engineered Vesicles for Perchlorate Degradation","abstract":"This study demonstrates the use of engineered vesicles to reduce perchlorate. Specifically, cell-free extracts containing perchlorate reductase and chlorite dismutase enzymes were encapsulated in a triblock copolymer vesicle functionalized with the outer membrane porin OmpF. The porin allows for perchlorate transport into the vesicles, inside which the encapsulated enzymes transform perchlorate to chloride. Perchlorate reduction was quantified using a methyl viologen colorimetric technique. The vesicle solutions had perchlorate-reducing activities ranging from 35-45 units per liter. This work shows that vesicles can provide a mechanism to utilize environmentally-relevant biological enzymes. When incorporated into a vesicle, the enzymes could be used outside of environmental conditions where they would normally be expressed by natural bacteria.","abstract_html":"This study demonstrates the use of engineered vesicles to reduce perchlorate. Specifically, cell-free extracts containing perchlorate reductase and chlorite dismutase enzymes were encapsulated in a triblock copolymer vesicle functionalized with the outer membrane porin OmpF. The porin allows for perchlorate transport into the vesicles, inside which the encapsulated enzymes transform perchlorate to chloride. Perchlorate reduction was quantified using a methyl viologen colorimetric technique. The vesicle solutions had perchlorate-reducing activities ranging from 35-45 units per liter. This work shows that vesicles can provide a mechanism to utilize environmentally-relevant biological enzymes. When incorporated into a vesicle, the enzymes could be used outside of environmental conditions where they would normally be expressed by natural bacteria.","abstract_has_math":false,"creators":["Poust, Sean K."],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Environ Engr in Civil Engr","degree_department":null,"school":null,"contributors":["Zilles, Julie L."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010-08-31T20:31:10Z","date_published":"2010-08-31T20:31:10Z","updated_at":"2026-07-22T22:25:09Z","subjects":["Polymer vesicles","Perchlorate treatment","Synthetic biology","Water treatment"],"languages":["en"],"rights":["Copyright 2010 Sean K. Poust"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/17064","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Zilles, Julie L."]},{"key":"dc:creator","label":"Author","values":["Poust, Sean K."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2010-08-31T20:31:10Z","2012-09-07T16:43:40Z","2010-08"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Environ Engr in Civil Engr"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Polymer vesicles","Perchlorate treatment","Synthetic biology","Water treatment"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2010 Sean K. Poust"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/17064"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["This study demonstrates the use of engineered vesicles to reduce perchlorate. Specifically, cell-free extracts containing perchlorate reductase and chlorite dismutase enzymes were encapsulated in a triblock copolymer vesicle functionalized with the outer membrane porin OmpF. The porin allows for perchlorate transport into the vesicles, inside which the encapsulated enzymes transform perchlorate to chloride. Perchlorate reduction was quantified using a methyl viologen colorimetric technique. The vesicle solutions had perchlorate-reducing activities ranging from 35-45 units per liter. This work shows that vesicles can provide a mechanism to utilize environmentally-relevant biological enzymes. When incorporated into a vesicle, the enzymes could be used outside of environmental conditions where they would normally be expressed by natural bacteria.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2010-07-21T22:37:40Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Poust_Sean.pdf: 1845282 bytes, checksum: 2de59c765454fc4f52370dcd94ad9fd4 (MD5) Thesis Draft Repaired.docx: 20117161 bytes, checksum: 7fb29d315c36725b7065d3251835c42a (MD5)","Made available in DSpace on 2010-08-31T20:31:10Z (GMT). No. of bitstreams: 3 Poust_Sean.pdf: 1845282 bytes, checksum: 2de59c765454fc4f52370dcd94ad9fd4 (MD5) Thesis Draft Repaired.docx: 20117161 bytes, checksum: 7fb29d315c36725b7065d3251835c42a (MD5) license.txt: 4057 bytes, checksum: 5fe31469e97532d29b845bb5dd48e650 (MD5)","Item marked as restricted to the 'Administrator' Group (id=1) by William Ingram (wingram2@illinois.edu) on 2010-08-31T20:32:56Z Item is restricted until 2012-08-31T20:32:49Z","Item reinstated by Sarah Shreeves (sshreeve@illinois.edu) on 2012-09-07T16:43:40Z Item was in collections: University of Illinois Dissertations and Theses (ID: 204) Dissertations and Theses - Civil and Environmental Engineering (ID: 672) No. of bitstreams: 3 Poust_Sean.pdf: 1845282 bytes, checksum: 2de59c765454fc4f52370dcd94ad9fd4 (MD5) Thesis Draft Repaired.docx: 20117161 bytes, checksum: 7fb29d315c36725b7065d3251835c42a (MD5) license.txt: 4057 bytes, checksum: 5fe31469e97532d29b845bb5dd48e650 (MD5)","Item released from any restrictions by Sarah Shreeves (sshreeve@illinois.edu) on 2012-09-07T16:43:40Z"]},{"key":"dc:title","label":"Title","values":["Engineered Vesicles for Perchlorate Degradation"]}]}],"canonical_facts":{"dc:contributor":["Zilles, Julie L."],"dc:creator":["Poust, Sean K."],"dc:date":["2010-08-31T20:31:10Z","2012-09-07T16:43:40Z","2010-08"],"dc:description":["This study demonstrates the use of engineered vesicles to reduce perchlorate. Specifically, cell-free extracts containing perchlorate reductase and chlorite dismutase enzymes were encapsulated in a triblock copolymer vesicle functionalized with the outer membrane porin OmpF. The porin allows for perchlorate transport into the vesicles, inside which the encapsulated enzymes transform perchlorate to chloride. Perchlorate reduction was quantified using a methyl viologen colorimetric technique. The vesicle solutions had perchlorate-reducing activities ranging from 35-45 units per liter. This work shows that vesicles can provide a mechanism to utilize environmentally-relevant biological enzymes. When incorporated into a vesicle, the enzymes could be used outside of environmental conditions where they would normally be expressed by natural bacteria.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2010-07-21T22:37:40Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Poust_Sean.pdf: 1845282 bytes, checksum: 2de59c765454fc4f52370dcd94ad9fd4 (MD5) Thesis Draft Repaired.docx: 20117161 bytes, checksum: 7fb29d315c36725b7065d3251835c42a (MD5)","Made available in DSpace on 2010-08-31T20:31:10Z (GMT). No. of bitstreams: 3 Poust_Sean.pdf: 1845282 bytes, checksum: 2de59c765454fc4f52370dcd94ad9fd4 (MD5) Thesis Draft Repaired.docx: 20117161 bytes, checksum: 7fb29d315c36725b7065d3251835c42a (MD5) license.txt: 4057 bytes, checksum: 5fe31469e97532d29b845bb5dd48e650 (MD5)","Item marked as restricted to the 'Administrator' Group (id=1) by William Ingram (wingram2@illinois.edu) on 2010-08-31T20:32:56Z Item is restricted until 2012-08-31T20:32:49Z","Item reinstated by Sarah Shreeves (sshreeve@illinois.edu) on 2012-09-07T16:43:40Z Item was in collections: University of Illinois Dissertations and Theses (ID: 204) Dissertations and Theses - Civil and Environmental Engineering (ID: 672) No. of bitstreams: 3 Poust_Sean.pdf: 1845282 bytes, checksum: 2de59c765454fc4f52370dcd94ad9fd4 (MD5) Thesis Draft Repaired.docx: 20117161 bytes, checksum: 7fb29d315c36725b7065d3251835c42a (MD5) license.txt: 4057 bytes, checksum: 5fe31469e97532d29b845bb5dd48e650 (MD5)","Item released from any restrictions by Sarah Shreeves (sshreeve@illinois.edu) on 2012-09-07T16:43:40Z"],"dc:identifier":["http://hdl.handle.net/2142/17064"],"dc:language":["en"],"dc:rights":["Copyright 2010 Sean K. Poust"],"dc:subject":["Polymer vesicles","Perchlorate treatment","Synthetic biology","Water treatment"],"dc:title":["Engineered Vesicles for Perchlorate Degradation"],"thesis:degree_discipline":["Environ Engr in Civil Engr"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:09Z"}