{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/16963"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/16963","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Characterization and modeling of cell activation upon TGF-β1 stimulation in a 3-D culture system","abstract":"In this work, we explored a thick in vitro 3-D cell culture model with layered structure and applied transforming growth factor-beta1 (TGF-β1) on the top of the 3-D culture to induce the conversion from normal fibroblasts to myofibroblasts and expression of a marker protein, alpha-smooth muscle actin (α-SMA). The cell response with TGF-β1 stimulation was captured with immunofluorescence staining (IF) and confocal microscopy imaging of α-SMA in each cell embedded layer. Our results revealed the spatial-temporal profiles of the conversion from normal fibroblasts to myofibroblasts in 3-D culture upon TGF-β1stimulation. The conversion starting time, speed and saturation plateau are closely related to TGF-β1 concentration. Based on these experimental results, we successfully developed a mathematical model, which incorporates diffusion of growth factors and cell activation responses to describe the dynamic cell response to growth factors across the 3-D culture depth and predict the spatial-temporal profile of the fibroblast conversion within the culture. In summary, we present here a handy experimental and computational tool for researches on tumor signaling pathways, and provide some guidance for studies on diffusion of protein factors or drugs in 3-D tissue environment.","abstract_html":"In this work, we explored a thick in vitro 3-D cell culture model with layered structure and applied transforming growth factor-beta1 (TGF-β1) on the top of the 3-D culture to induce the conversion from normal fibroblasts to myofibroblasts and expression of a marker protein, alpha-smooth muscle actin (α-SMA). The cell response with TGF-β1 stimulation was captured with immunofluorescence staining (IF) and confocal microscopy imaging of α-SMA in each cell embedded layer. Our results revealed the spatial-temporal profiles of the conversion from normal fibroblasts to myofibroblasts in 3-D culture upon TGF-β1stimulation. The conversion starting time, speed and saturation plateau are closely related to TGF-β1 concentration. Based on these experimental results, we successfully developed a mathematical model, which incorporates diffusion of growth factors and cell activation responses to describe the dynamic cell response to growth factors across the 3-D culture depth and predict the spatial-temporal profile of the fibroblast conversion within the culture. In summary, we present here a handy experimental and computational tool for researches on tumor signaling pathways, and provide some guidance for studies on diffusion of protein factors or drugs in 3-D tissue environment.","abstract_has_math":false,"creators":["Xu, Jing"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Bioengineering","degree_department":null,"school":null,"contributors":["Insana, Michael F."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010-08-31T20:02:23Z","date_published":"2010-08-31T20:02:23Z","updated_at":"2026-07-22T22:25:09Z","subjects":["myofibroblasts","transforming growth factor-beta1","alpha-smooth muscle actin","diffusion","confocal microscopy imaging","immunofluorescence staining"],"languages":["en"],"rights":["Copyright 2010 Jing Xu"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/16963","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Insana, Michael F."]},{"key":"dc:creator","label":"Author","values":["Xu, Jing"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2010-08-31T20:02:23Z","2012-09-07T16:43:32Z","2010-08"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Bioengineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["myofibroblasts","transforming growth factor-beta1","alpha-smooth muscle actin","diffusion","confocal microscopy imaging","immunofluorescence staining"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2010 Jing Xu"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/16963"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["In this work, we explored a thick in vitro 3-D cell culture model with layered structure and applied transforming growth factor-beta1 (TGF-β1) on the top of the 3-D culture to induce the conversion from normal fibroblasts to myofibroblasts and expression of a marker protein, alpha-smooth muscle actin (α-SMA). The cell response with TGF-β1 stimulation was captured with immunofluorescence staining (IF) and confocal microscopy imaging of α-SMA in each cell embedded layer. Our results revealed the spatial-temporal profiles of the conversion from normal fibroblasts to myofibroblasts in 3-D culture upon TGF-β1stimulation. The conversion starting time, speed and saturation plateau are closely related to TGF-β1 concentration. Based on these experimental results, we successfully developed a mathematical model, which incorporates diffusion of growth factors and cell activation responses to describe the dynamic cell response to growth factors across the 3-D culture depth and predict the spatial-temporal profile of the fibroblast conversion within the culture. 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The cell response with TGF-β1 stimulation was captured with immunofluorescence staining (IF) and confocal microscopy imaging of α-SMA in each cell embedded layer. Our results revealed the spatial-temporal profiles of the conversion from normal fibroblasts to myofibroblasts in 3-D culture upon TGF-β1stimulation. The conversion starting time, speed and saturation plateau are closely related to TGF-β1 concentration. Based on these experimental results, we successfully developed a mathematical model, which incorporates diffusion of growth factors and cell activation responses to describe the dynamic cell response to growth factors across the 3-D culture depth and predict the spatial-temporal profile of the fibroblast conversion within the culture. 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