{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/16000"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/16000","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"The α7β1 integrin accelerates exercise-induced myogenesis","abstract":"Muscle-specific transgenic overexpression of the α7β1 integrin can protect against exercise induced-injury and promote the appearance of multipotent mesenchymal-like (Sca-1+CD45-) stem cells in skeletal muscle. PURPOSE: The purpose of this study was to determine if overexpression of the α7β1 integrin also results in enhanced and/or accelerated myogenesis following a single bout of eccentric exercise. METHODS: 5-wk old, female wild type (WT) and α7 integrin transgenic (α7Tg) mice completed a single bout of downhill running exercise (-20o, 17 m/min, 60 min) and gastrocnemius-soleus complexes were collected 2, 4, and 7 d post-exercise. Myogenesis was quantified using embryonic myosin heavy chain (eMHC) expression and the presence of centrally located nuclei (CLN). RESULTS: Whereas gradual increases in eMHC expression were detected in WT mice following exercise, a 5-fold increase in eMHC+ fibers was present at 2 d post-exercise in α7Tg mice compared to α7Tg mice that did not exercise (P < 0.001). Consistent with this adaptation, WT mice had gradual increases in numbers of CLN until 7 d post-exercise, whereas α7Tg mice had a 5-fold increase in CLN levels at 2 d post-exercise (P<0.001). CONCLUSION: This study suggests that the protection from injury and increased appearance of mesenchymal stem cells observed with α7β1 integrin overexpression may directly or indirectly contribute to accelerated myogenesis following eccentric exercise. This study provides the first evidence that the α7β1 integrin participates in myogenesis acceleration.","abstract_html":"Muscle-specific transgenic overexpression of the α7β1 integrin can protect against exercise induced-injury and promote the appearance of multipotent mesenchymal-like (Sca-1+CD45-) stem cells in skeletal muscle. PURPOSE: The purpose of this study was to determine if overexpression of the α7β1 integrin also results in enhanced and/or accelerated myogenesis following a single bout of eccentric exercise. METHODS: 5-wk old, female wild type (WT) and α7 integrin transgenic (α7Tg) mice completed a single bout of downhill running exercise (-20o, 17 m/min, 60 min) and gastrocnemius-soleus complexes were collected 2, 4, and 7 d post-exercise. Myogenesis was quantified using embryonic myosin heavy chain (eMHC) expression and the presence of centrally located nuclei (CLN). RESULTS: Whereas gradual increases in eMHC expression were detected in WT mice following exercise, a 5-fold increase in eMHC+ fibers was present at 2 d post-exercise in α7Tg mice compared to α7Tg mice that did not exercise (P &lt; 0.001). Consistent with this adaptation, WT mice had gradual increases in numbers of CLN until 7 d post-exercise, whereas α7Tg mice had a 5-fold increase in CLN levels at 2 d post-exercise (P&lt;0.001). CONCLUSION: This study suggests that the protection from injury and increased appearance of mesenchymal stem cells observed with α7β1 integrin overexpression may directly or indirectly contribute to accelerated myogenesis following eccentric exercise. This study provides the first evidence that the α7β1 integrin participates in myogenesis acceleration.","abstract_has_math":false,"creators":["Lueders, Tara N."],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Kinesiology","degree_department":null,"school":null,"contributors":["Boppart, Marni D."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010-05-18T18:55:41Z","date_published":"2010-05-18T18:55:41Z","updated_at":"2026-07-22T22:25:08Z","subjects":["myogenesis","eccentric exercise","alpha7beta1 integrin","mesenchymal-like stem cells","Sca-1+","CD45-"],"languages":["en"],"rights":["Copyright 2010 Tara N. Lueders"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/16000","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Boppart, Marni D."]},{"key":"dc:creator","label":"Author","values":["Lueders, Tara N."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2010-05-18T18:55:41Z","2012-05-19T10:00:16Z","2010-5"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Kinesiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["myogenesis","eccentric exercise","alpha7beta1 integrin","mesenchymal-like stem cells","Sca-1+","CD45-"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2010 Tara N. Lueders"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/16000"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Muscle-specific transgenic overexpression of the α7β1 integrin can protect against exercise induced-injury and promote the appearance of multipotent mesenchymal-like (Sca-1+CD45-) stem cells in skeletal muscle. PURPOSE: The purpose of this study was to determine if overexpression of the α7β1 integrin also results in enhanced and/or accelerated myogenesis following a single bout of eccentric exercise. METHODS: 5-wk old, female wild type (WT) and α7 integrin transgenic (α7Tg) mice completed a single bout of downhill running exercise (-20o, 17 m/min, 60 min) and gastrocnemius-soleus complexes were collected 2, 4, and 7 d post-exercise. Myogenesis was quantified using embryonic myosin heavy chain (eMHC) expression and the presence of centrally located nuclei (CLN). RESULTS: Whereas gradual increases in eMHC expression were detected in WT mice following exercise, a 5-fold increase in eMHC+ fibers was present at 2 d post-exercise in α7Tg mice compared to α7Tg mice that did not exercise (P < 0.001). Consistent with this adaptation, WT mice had gradual increases in numbers of CLN until 7 d post-exercise, whereas α7Tg mice had a 5-fold increase in CLN levels at 2 d post-exercise (P<0.001). CONCLUSION: This study suggests that the protection from injury and increased appearance of mesenchymal stem cells observed with α7β1 integrin overexpression may directly or indirectly contribute to accelerated myogenesis following eccentric exercise. This study provides the first evidence that the α7β1 integrin participates in myogenesis acceleration.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2010-04-29T21:17:07Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Lueders_Tara.docx: 9724872 bytes, checksum: 0724d025d3161070b5172a391872dbb5 (MD5) Lueders_Tara.pdf: 3516598 bytes, checksum: 60d8fe63316e8d32b1d855855a76806c (MD5)","Made available in DSpace on 2010-05-18T18:55:41Z (GMT). No. of bitstreams: 3 Lueders_Tara.docx: 9724872 bytes, checksum: 0724d025d3161070b5172a391872dbb5 (MD5) Lueders_Tara.pdf: 3516598 bytes, checksum: 60d8fe63316e8d32b1d855855a76806c (MD5) license.txt: 4062 bytes, checksum: 3f783f218b5968246c1c853ec237b585 (MD5)","Item marked as restricted to the 'Administrator' Group (id=1) by William Ingram (wingram2@illinois.edu) on 2010-05-18T18:56:43Z Item is restricted until 2012-05-18T18:56:42Z","Item reinstated by Sarah Shreeves (sshreeve@illinois.edu) on 2012-05-19T10:00:16Z Item was in collections: Dissertations and Theses - Kinesiology and Community Health (ID: 740) University of Illinois Dissertations and Theses (ID: 204) No. of bitstreams: 4 Lueders_Tara.pdf.txt: 113504 bytes, checksum: 1fd73121c4c194731281d149c7c0ddde (MD5) Lueders_Tara.docx: 9724872 bytes, checksum: 0724d025d3161070b5172a391872dbb5 (MD5) Lueders_Tara.pdf: 3516598 bytes, checksum: 60d8fe63316e8d32b1d855855a76806c (MD5) license.txt: 4062 bytes, checksum: 3f783f218b5968246c1c853ec237b585 (MD5)","Item released from any restrictions by Sarah Shreeves (sshreeve@illinois.edu) on 2012-05-19T10:00:16Z"]},{"key":"dc:title","label":"Title","values":["The α7β1 integrin accelerates exercise-induced myogenesis"]}]}],"canonical_facts":{"dc:contributor":["Boppart, Marni D."],"dc:creator":["Lueders, Tara N."],"dc:date":["2010-05-18T18:55:41Z","2012-05-19T10:00:16Z","2010-5"],"dc:description":["Muscle-specific transgenic overexpression of the α7β1 integrin can protect against exercise induced-injury and promote the appearance of multipotent mesenchymal-like (Sca-1+CD45-) stem cells in skeletal muscle. PURPOSE: The purpose of this study was to determine if overexpression of the α7β1 integrin also results in enhanced and/or accelerated myogenesis following a single bout of eccentric exercise. METHODS: 5-wk old, female wild type (WT) and α7 integrin transgenic (α7Tg) mice completed a single bout of downhill running exercise (-20o, 17 m/min, 60 min) and gastrocnemius-soleus complexes were collected 2, 4, and 7 d post-exercise. Myogenesis was quantified using embryonic myosin heavy chain (eMHC) expression and the presence of centrally located nuclei (CLN). RESULTS: Whereas gradual increases in eMHC expression were detected in WT mice following exercise, a 5-fold increase in eMHC+ fibers was present at 2 d post-exercise in α7Tg mice compared to α7Tg mice that did not exercise (P < 0.001). Consistent with this adaptation, WT mice had gradual increases in numbers of CLN until 7 d post-exercise, whereas α7Tg mice had a 5-fold increase in CLN levels at 2 d post-exercise (P<0.001). CONCLUSION: This study suggests that the protection from injury and increased appearance of mesenchymal stem cells observed with α7β1 integrin overexpression may directly or indirectly contribute to accelerated myogenesis following eccentric exercise. This study provides the first evidence that the α7β1 integrin participates in myogenesis acceleration.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2010-04-29T21:17:07Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Lueders_Tara.docx: 9724872 bytes, checksum: 0724d025d3161070b5172a391872dbb5 (MD5) Lueders_Tara.pdf: 3516598 bytes, checksum: 60d8fe63316e8d32b1d855855a76806c (MD5)","Made available in DSpace on 2010-05-18T18:55:41Z (GMT). No. of bitstreams: 3 Lueders_Tara.docx: 9724872 bytes, checksum: 0724d025d3161070b5172a391872dbb5 (MD5) Lueders_Tara.pdf: 3516598 bytes, checksum: 60d8fe63316e8d32b1d855855a76806c (MD5) license.txt: 4062 bytes, checksum: 3f783f218b5968246c1c853ec237b585 (MD5)","Item marked as restricted to the 'Administrator' Group (id=1) by William Ingram (wingram2@illinois.edu) on 2010-05-18T18:56:43Z Item is restricted until 2012-05-18T18:56:42Z","Item reinstated by Sarah Shreeves (sshreeve@illinois.edu) on 2012-05-19T10:00:16Z Item was in collections: Dissertations and Theses - Kinesiology and Community Health (ID: 740) University of Illinois Dissertations and Theses (ID: 204) No. of bitstreams: 4 Lueders_Tara.pdf.txt: 113504 bytes, checksum: 1fd73121c4c194731281d149c7c0ddde (MD5) Lueders_Tara.docx: 9724872 bytes, checksum: 0724d025d3161070b5172a391872dbb5 (MD5) Lueders_Tara.pdf: 3516598 bytes, checksum: 60d8fe63316e8d32b1d855855a76806c (MD5) license.txt: 4062 bytes, checksum: 3f783f218b5968246c1c853ec237b585 (MD5)","Item released from any restrictions by Sarah Shreeves (sshreeve@illinois.edu) on 2012-05-19T10:00:16Z"],"dc:identifier":["http://hdl.handle.net/2142/16000"],"dc:language":["en"],"dc:rights":["Copyright 2010 Tara N. Lueders"],"dc:subject":["myogenesis","eccentric exercise","alpha7beta1 integrin","mesenchymal-like stem cells","Sca-1+","CD45-"],"dc:title":["The α7β1 integrin accelerates exercise-induced myogenesis"],"thesis:degree_discipline":["Kinesiology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:08Z"}