{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/130098"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/130098","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Photonic resonator absorption microscopy for dynamic point of care biosensing","abstract":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-08-01","abstract_html":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-08-01","abstract_has_math":false,"creators":["Liu, Weinan"],"institution":"University of Illinois Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Electrical & Computer Engr","degree_department":null,"school":null,"contributors":["Cunningham, Brian T.","Do, Minh N.","Gruev, Viktor","Zhao, Yang"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-07-18","date_published":"2025-07-18","updated_at":"2026-07-22T22:25:06Z","subjects":["Digital-resolution Biosensing","Photonic Crystal","Point-of-care","Microscopy","Metasurface","Computational Imaging","Biomedical Detection"],"languages":["en","eng"],"rights":["Copyright 2025 Weinan Liu"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/130098","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Cunningham, Brian T.","Do, Minh N.","Gruev, Viktor","Zhao, Yang"]},{"key":"dc:creator","label":"Author","values":["Liu, Weinan"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2025-07-18","2025-08"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Electrical & Computer Engr"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Digital-resolution Biosensing","Photonic Crystal","Point-of-care","Microscopy","Metasurface","Computational Imaging","Biomedical Detection"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2025 Weinan Liu"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/130098"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-08-01","The student, Weinan Liu, accepted the attached license on 2025-07-17 at 19:10.","The student, Weinan Liu, submitted this Dissertation for approval on 2025-07-17 at 19:13.","This Dissertation was approved for publication on 2025-07-18 at 14:55.","DSpace SAF Submission Ingestion Package generated from Vireo submission #22563 on 2025-10-25 at 15:31:00","This dissertation introduces Photonic Resonator Absorption Microscopy (PRAM), a label-free, digital-resolution biosensing platform designed for highly sensitive and accessible point-of-care diagnostics. By coupling photonic crystal (PC) surfaces with gold nanoparticle (AuNP) tags, PRAM enables attomolar detection of biomolecules without enzymatic amplification or complex workflows. To advance PRAM for real-world applications, several innovations are presented. A portable, automated PRAM system (ap-PRAM) integrates motorized autofocus, large field-of-view (FOV) tiling, and a differential dynamic detection algorithm to enable real-time, single-particle kinetic analysis of binding and unbinding events. These capabilities are demonstrated in both sandwich immunoassays for viral antibodies (HIV, HCV) and a target recycling amplification assay for miRNA, achieving detection limits as low as 1 aM. In parallel, a miniaturized PRAM system (PRAM Mini) is developed, featuring compact optics, smartphone integration, and digital image processing for decentralized or resource-limited settings. To further enhance signal spatial uniformity and polarization independence, a two-dimensional PC substrate is designed and fabricated, improving signal consistency across the sensor surface. Together, these developments establish PRAM as a versatile and scalable platform with broad potential for clinical diagnostics, liquid biopsy, and dynamic biosensing in both laboratory and point-of-care environments."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Photonic resonator absorption microscopy for dynamic point of care biosensing"]}]}],"canonical_facts":{"dc:contributor":["Cunningham, Brian T.","Do, Minh N.","Gruev, Viktor","Zhao, Yang"],"dc:creator":["Liu, Weinan"],"dc:date":["2025-07-18","2025-08"],"dc:description":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-08-01","The student, Weinan Liu, accepted the attached license on 2025-07-17 at 19:10.","The student, Weinan Liu, submitted this Dissertation for approval on 2025-07-17 at 19:13.","This Dissertation was approved for publication on 2025-07-18 at 14:55.","DSpace SAF Submission Ingestion Package generated from Vireo submission #22563 on 2025-10-25 at 15:31:00","This dissertation introduces Photonic Resonator Absorption Microscopy (PRAM), a label-free, digital-resolution biosensing platform designed for highly sensitive and accessible point-of-care diagnostics. By coupling photonic crystal (PC) surfaces with gold nanoparticle (AuNP) tags, PRAM enables attomolar detection of biomolecules without enzymatic amplification or complex workflows. To advance PRAM for real-world applications, several innovations are presented. A portable, automated PRAM system (ap-PRAM) integrates motorized autofocus, large field-of-view (FOV) tiling, and a differential dynamic detection algorithm to enable real-time, single-particle kinetic analysis of binding and unbinding events. These capabilities are demonstrated in both sandwich immunoassays for viral antibodies (HIV, HCV) and a target recycling amplification assay for miRNA, achieving detection limits as low as 1 aM. In parallel, a miniaturized PRAM system (PRAM Mini) is developed, featuring compact optics, smartphone integration, and digital image processing for decentralized or resource-limited settings. To further enhance signal spatial uniformity and polarization independence, a two-dimensional PC substrate is designed and fabricated, improving signal consistency across the sensor surface. Together, these developments establish PRAM as a versatile and scalable platform with broad potential for clinical diagnostics, liquid biopsy, and dynamic biosensing in both laboratory and point-of-care environments."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/130098"],"dc:language":["en","eng"],"dc:rights":["Copyright 2025 Weinan Liu"],"dc:subject":["Digital-resolution Biosensing","Photonic Crystal","Point-of-care","Microscopy","Metasurface","Computational Imaging","Biomedical Detection"],"dc:title":["Photonic resonator absorption microscopy for dynamic point of care biosensing"],"dc:type":["text"],"thesis:degree_discipline":["Electrical & Computer Engr"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:06Z"}