{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/129571"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/129571","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Label-free optical signatures of extracellular vesicles and their applications in disease diagnosis and therapy response","abstract":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-05-01","abstract_html":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-05-01","abstract_has_math":false,"creators":["Park, Jaena"],"institution":"University of Illinois Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Bioengineering","degree_department":null,"school":null,"contributors":["Boppart, Stephen A","Boppart, Stephen A.","Cunningham, Brian T.","Dobrucki, Wawrzyniec","Selting, Kimberly A."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-04-25","date_published":"2025-04-25","updated_at":"2026-07-22T22:25:05Z","subjects":["Extracellular Vesicles","Label-Free Imaging","Multiphoton Microscopy","Optical Redox Ratio","Cancer Diagnosis","Therapy Response Monitoring","Urinary Biomarkers","Metabolic Profiling","Tumor Microenvironment"],"languages":["en","eng"],"rights":["Copyright 2025 Jaena Park"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/129571","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Boppart, Stephen A","Boppart, Stephen A.","Cunningham, Brian T.","Dobrucki, Wawrzyniec","Selting, Kimberly A."]},{"key":"dc:creator","label":"Author","values":["Park, Jaena"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2025-04-25","2025-05"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Bioengineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Extracellular Vesicles","Label-Free Imaging","Multiphoton Microscopy","Optical Redox Ratio","Cancer Diagnosis","Therapy Response Monitoring","Urinary Biomarkers","Metabolic Profiling","Tumor Microenvironment"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2025 Jaena Park"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/129571"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-05-01","The student, Jaena Park, accepted the attached license on 2025-04-24 at 12:24.","The student, Jaena Park, submitted this Dissertation for approval on 2025-04-24 at 12:27.","This Dissertation was approved for publication on 2025-04-25 at 11:43.","DSpace SAF Submission Ingestion Package generated from Vireo submission #21931 on 2025-10-19 at 19:16:06","Extracellular vesicles (EVs) have emerged as critical mediators of cell-to-cell communication, particularly in the tumor microenvironment, where they modulate hypoxia response, drug resistance, and metastatic progression. Beyond these roles, EVs are promising candidates for noninvasive cancer diagnostics and therapy monitoring through liquid biopsy. However, a fundamental barrier to clinical translation lies in the difficulty of characterizing EVs in situ while preserving their biochemical composition, spatial origin, and functional state. Traditional EV analyses often rely on bulk or destructive molecular profiling, limiting their diagnostic specificity and obscuring biologically relevant heterogeneity. More critically, it remains unclear whether the metabolic content of EVs reflects the state of their parental cells. To address these challenges, this thesis integrates label-free intravital and ex vivo optical imaging to investigate the metabolic characteristics of EVs across multiple oncologic conditions, including hypoxia, chemotherapy, and radiotherapy. Simultaneous label-free autofluorescence-multiharmonic (SLAM) microscopy enables high-resolution imaging and real-time metabolic profiling of EVs using endogenous optical contrast from coenzymes found in EVs. Across tumor models and biofluid-derived EV populations, optical redox ratio (ORR) imaging was used to assess metabolic reprogramming associated with disease state and treatment response. The findings demonstrate that autofluorescence-based EV profiling supports noninvasive, functionally informative liquid biopsy for cancer screening, prognostication, and therapy assessment by capturing dynamic metabolic shifts reflective of parent cell states."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Label-free optical signatures of extracellular vesicles and their applications in disease diagnosis and therapy response"]}]}],"canonical_facts":{"dc:contributor":["Boppart, Stephen A","Boppart, Stephen A.","Cunningham, Brian T.","Dobrucki, Wawrzyniec","Selting, Kimberly A."],"dc:creator":["Park, Jaena"],"dc:date":["2025-04-25","2025-05"],"dc:description":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-05-01","The student, Jaena Park, accepted the attached license on 2025-04-24 at 12:24.","The student, Jaena Park, submitted this Dissertation for approval on 2025-04-24 at 12:27.","This Dissertation was approved for publication on 2025-04-25 at 11:43.","DSpace SAF Submission Ingestion Package generated from Vireo submission #21931 on 2025-10-19 at 19:16:06","Extracellular vesicles (EVs) have emerged as critical mediators of cell-to-cell communication, particularly in the tumor microenvironment, where they modulate hypoxia response, drug resistance, and metastatic progression. Beyond these roles, EVs are promising candidates for noninvasive cancer diagnostics and therapy monitoring through liquid biopsy. However, a fundamental barrier to clinical translation lies in the difficulty of characterizing EVs in situ while preserving their biochemical composition, spatial origin, and functional state. Traditional EV analyses often rely on bulk or destructive molecular profiling, limiting their diagnostic specificity and obscuring biologically relevant heterogeneity. More critically, it remains unclear whether the metabolic content of EVs reflects the state of their parental cells. To address these challenges, this thesis integrates label-free intravital and ex vivo optical imaging to investigate the metabolic characteristics of EVs across multiple oncologic conditions, including hypoxia, chemotherapy, and radiotherapy. Simultaneous label-free autofluorescence-multiharmonic (SLAM) microscopy enables high-resolution imaging and real-time metabolic profiling of EVs using endogenous optical contrast from coenzymes found in EVs. Across tumor models and biofluid-derived EV populations, optical redox ratio (ORR) imaging was used to assess metabolic reprogramming associated with disease state and treatment response. The findings demonstrate that autofluorescence-based EV profiling supports noninvasive, functionally informative liquid biopsy for cancer screening, prognostication, and therapy assessment by capturing dynamic metabolic shifts reflective of parent cell states."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/129571"],"dc:language":["en","eng"],"dc:rights":["Copyright 2025 Jaena Park"],"dc:subject":["Extracellular Vesicles","Label-Free Imaging","Multiphoton Microscopy","Optical Redox Ratio","Cancer Diagnosis","Therapy Response Monitoring","Urinary Biomarkers","Metabolic Profiling","Tumor Microenvironment"],"dc:title":["Label-free optical signatures of extracellular vesicles and their applications in disease diagnosis and therapy response"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Bioengineering"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:05Z"}