{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/129564"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/129564","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Dynamic imaging of magnetic bioeffects in cells using two-channel two-photon autofluorescence intensity and lifetime microscopy","abstract":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-05-01","abstract_html":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-05-01","abstract_has_math":false,"creators":["Tan, Kevin Kaipeng Durfee"],"institution":"University of Illinois Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Bioengineering","degree_department":null,"school":null,"contributors":["Boppart, Stephen A"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-05-05","date_published":"2025-05-05","updated_at":"2026-07-22T22:25:05Z","subjects":["Two-photon microscopy","Autofluorescence microscopy","Label-free imaging","Quantum biology","Magnetic bioeffects","Radical pair mechanism","Reactive oxygen species","Fluorescence lifetime imaging microscopy","Spin chemistry","Metabolic imaging"],"languages":["en","eng"],"rights":["© 2025 Kevin Kaipeng Durfee Tan"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/129564","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Boppart, Stephen A"]},{"key":"dc:creator","label":"Author","values":["Tan, Kevin Kaipeng Durfee"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2025-05-05","2025-05"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Bioengineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Two-photon microscopy","Autofluorescence microscopy","Label-free imaging","Quantum biology","Magnetic bioeffects","Radical pair mechanism","Reactive oxygen species","Fluorescence lifetime imaging microscopy","Spin chemistry","Metabolic imaging"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["© 2025 Kevin Kaipeng Durfee Tan"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/129564"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-05-01","The student, Kevin Tan, accepted the attached license on 2025-04-28 at 14:03.","The student, Kevin Tan, submitted this Thesis for approval on 2025-04-28 at 16:15.","This Thesis was approved for publication on 2025-05-05 at 09:10.","DSpace SAF Submission Ingestion Package generated from Vireo submission #21910 on 2025-10-19 at 19:15:26","Magnetic fields have long been known to interact with living systems in subtle and often surprising ways. One hypothesis for these effects is the radical pair mechanism which suggests that magnetic fields influence the spin dynamics of redox-active radical pairs, altering the balance of reactive oxygen species (ROS) in cells. These ROS may then initiate signaling and metabolic pathways. In this thesis, a novel two-channel two-photon autofluorescence microscope enabled high-resolution, non-invasive, and simultaneous measurement of autofluorescence intensity and fluorescence lifetime from the metabolic cofactors NAD(P)H and FAD to examine magnetic field effects at the single-cell level. First, A549 lung carcinoma cells were subjected to the redox effects of PEG-SOD and DDC to validate the sensitivity of the microscopy method. While fluorometric assays confirmed altered ROS partitioning, autofluorescence microscopy exhibited limited sensitivity. Next, static magnetic fields ranging from 50 µT to 100 mT were applied for 72 hours. Fluorometric results revealed a significant increase in hydrogen peroxide production in the 0.4–0.8 mT range, consistent with the radical pair mechanism. The autofluorescence microscopy detected subtle changes in fluorescence lifetime features at 0.1 mT, indicating a possible NADPH mediated ROS defense response. Lastly, to probe the dynamics of magnetic bioeffects, a longitudinal imaging protocol including a custom stage-top Helmholtz coil was developed. No statistically significant differences were observed after repeated 2-hour magnetic field exposures, indicating limitations in the sensitivity of the label-free multiphoton imaging. Still, with adjustments, these methods are a promising tool for future investigations of magnetically-induced cellular phenomena."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Dynamic imaging of magnetic bioeffects in cells using two-channel two-photon autofluorescence intensity and lifetime microscopy"]}]}],"canonical_facts":{"dc:contributor":["Boppart, Stephen A"],"dc:creator":["Tan, Kevin Kaipeng Durfee"],"dc:date":["2025-05-05","2025-05"],"dc:description":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-05-01","The student, Kevin Tan, accepted the attached license on 2025-04-28 at 14:03.","The student, Kevin Tan, submitted this Thesis for approval on 2025-04-28 at 16:15.","This Thesis was approved for publication on 2025-05-05 at 09:10.","DSpace SAF Submission Ingestion Package generated from Vireo submission #21910 on 2025-10-19 at 19:15:26","Magnetic fields have long been known to interact with living systems in subtle and often surprising ways. One hypothesis for these effects is the radical pair mechanism which suggests that magnetic fields influence the spin dynamics of redox-active radical pairs, altering the balance of reactive oxygen species (ROS) in cells. These ROS may then initiate signaling and metabolic pathways. In this thesis, a novel two-channel two-photon autofluorescence microscope enabled high-resolution, non-invasive, and simultaneous measurement of autofluorescence intensity and fluorescence lifetime from the metabolic cofactors NAD(P)H and FAD to examine magnetic field effects at the single-cell level. First, A549 lung carcinoma cells were subjected to the redox effects of PEG-SOD and DDC to validate the sensitivity of the microscopy method. While fluorometric assays confirmed altered ROS partitioning, autofluorescence microscopy exhibited limited sensitivity. Next, static magnetic fields ranging from 50 µT to 100 mT were applied for 72 hours. Fluorometric results revealed a significant increase in hydrogen peroxide production in the 0.4–0.8 mT range, consistent with the radical pair mechanism. The autofluorescence microscopy detected subtle changes in fluorescence lifetime features at 0.1 mT, indicating a possible NADPH mediated ROS defense response. Lastly, to probe the dynamics of magnetic bioeffects, a longitudinal imaging protocol including a custom stage-top Helmholtz coil was developed. No statistically significant differences were observed after repeated 2-hour magnetic field exposures, indicating limitations in the sensitivity of the label-free multiphoton imaging. Still, with adjustments, these methods are a promising tool for future investigations of magnetically-induced cellular phenomena."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/129564"],"dc:language":["en","eng"],"dc:rights":["© 2025 Kevin Kaipeng Durfee Tan"],"dc:subject":["Two-photon microscopy","Autofluorescence microscopy","Label-free imaging","Quantum biology","Magnetic bioeffects","Radical pair mechanism","Reactive oxygen species","Fluorescence lifetime imaging microscopy","Spin chemistry","Metabolic imaging"],"dc:title":["Dynamic imaging of magnetic bioeffects in cells using two-channel two-photon autofluorescence intensity and lifetime microscopy"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Bioengineering"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:05Z"}