{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/129288"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/129288","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Elevated colorectal cancer risk in the hnRNP I knockout mice treated with azoxymethane/dextran sulfate sodium","abstract":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2025-10-19 without embargo terms","abstract_html":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2025-10-19 without embargo terms","abstract_has_math":false,"creators":["Zhang, Hehe"],"institution":"University of Illinois Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Food Science & Human Nutrition","degree_department":null,"school":null,"contributors":["Pan, Yuan-Xiang","Chen, Hong","Mei, Wenyan"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-05-06","date_published":"2025-05-06","updated_at":"2026-07-22T22:25:04Z","subjects":["hnRNP I Knockout Mice","early-stage tumorigenesis","AOM/DSS treatment"],"languages":["en","eng"],"rights":["Copyright 2025 Hehe Zhang"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/129288","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Pan, Yuan-Xiang","Chen, Hong","Mei, Wenyan"]},{"key":"dc:creator","label":"Author","values":["Zhang, Hehe"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2025-05-06","2025-05"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Food Science & Human Nutrition"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["hnRNP I Knockout Mice","early-stage tumorigenesis","AOM/DSS treatment"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2025 Hehe Zhang"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/129288"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2025-10-19 without embargo terms","The student, Hehe Zhang, accepted the attached license on 2025-04-30 at 12:26.","The student, Hehe Zhang, submitted this Thesis for approval on 2025-04-30 at 12:52.","This Thesis was approved for publication on 2025-05-06 at 16:47.","DSpace SAF Submission Ingestion Package generated from Vireo submission #22118 on 2025-10-19 at 18:11:23","Chronic inflammation and increased epithelial proliferation play an important role in the development of colorectal cancer (CRC), which is one of the most common and lethal cancers worldwide. HnRNP I is an RNA-binding protein that plays an important role in post-transcriptional regulation of gene expression and intestinal barrier function. In this study, hnRNP I knockout (KO) mice of and AOM/DSS chemical induction model were used to investigate the molecular mechanism of hnRNP I deficiency on colon epithelial dysplasia and early carcinogenesis. The results showed that the hnRNP I knockout mice showed significant weight loss and increased disease activity index (DAI) during AOM/DSS treatment. Abnormal crypt foci (ACF) and dysplasia were found in mice. In addition, elevated nuclear β-catenin localization was observed, strongly suggesting Wnt pathway induction. Moreover, the population of goblet cells was significantly decreased, indicating impaired epithelial barrier integrity and heightened mucosal injury. Ki-67 mislocalization indicated abnormal cell proliferation, which was associated with disruption of recess homeostasis. Our results provide strong evidence that loss of hnRNP I lead to a disruption of epithelial proliferation compartmentalization prevents secretory cell differentiation, and drives aberrant Wnt signaling. It indicates a strong correlation between this loss and the direct stimulation of colonic epithelial dysplasia and the initiation of tumors. Collectively, these findings indicate that hnRNP I is an important regulator of intestinal epithelial homeostasis and suppresses tumor development. These findings provide new insights into the molecular mechanisms of inflammation-driven colorectal cancer and establish a theoretical basis for potential intervention approaches that target the hnRNP I regulatory network to inhibit precancerous lesions."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Elevated colorectal cancer risk in the hnRNP I knockout mice treated with azoxymethane/dextran sulfate sodium"]}]}],"canonical_facts":{"dc:contributor":["Pan, Yuan-Xiang","Chen, Hong","Mei, Wenyan"],"dc:creator":["Zhang, Hehe"],"dc:date":["2025-05-06","2025-05"],"dc:description":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2025-10-19 without embargo terms","The student, Hehe Zhang, accepted the attached license on 2025-04-30 at 12:26.","The student, Hehe Zhang, submitted this Thesis for approval on 2025-04-30 at 12:52.","This Thesis was approved for publication on 2025-05-06 at 16:47.","DSpace SAF Submission Ingestion Package generated from Vireo submission #22118 on 2025-10-19 at 18:11:23","Chronic inflammation and increased epithelial proliferation play an important role in the development of colorectal cancer (CRC), which is one of the most common and lethal cancers worldwide. HnRNP I is an RNA-binding protein that plays an important role in post-transcriptional regulation of gene expression and intestinal barrier function. In this study, hnRNP I knockout (KO) mice of and AOM/DSS chemical induction model were used to investigate the molecular mechanism of hnRNP I deficiency on colon epithelial dysplasia and early carcinogenesis. The results showed that the hnRNP I knockout mice showed significant weight loss and increased disease activity index (DAI) during AOM/DSS treatment. Abnormal crypt foci (ACF) and dysplasia were found in mice. In addition, elevated nuclear β-catenin localization was observed, strongly suggesting Wnt pathway induction. Moreover, the population of goblet cells was significantly decreased, indicating impaired epithelial barrier integrity and heightened mucosal injury. Ki-67 mislocalization indicated abnormal cell proliferation, which was associated with disruption of recess homeostasis. Our results provide strong evidence that loss of hnRNP I lead to a disruption of epithelial proliferation compartmentalization prevents secretory cell differentiation, and drives aberrant Wnt signaling. It indicates a strong correlation between this loss and the direct stimulation of colonic epithelial dysplasia and the initiation of tumors. Collectively, these findings indicate that hnRNP I is an important regulator of intestinal epithelial homeostasis and suppresses tumor development. These findings provide new insights into the molecular mechanisms of inflammation-driven colorectal cancer and establish a theoretical basis for potential intervention approaches that target the hnRNP I regulatory network to inhibit precancerous lesions."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/129288"],"dc:language":["en","eng"],"dc:rights":["Copyright 2025 Hehe Zhang"],"dc:subject":["hnRNP I Knockout Mice","early-stage tumorigenesis","AOM/DSS treatment"],"dc:title":["Elevated colorectal cancer risk in the hnRNP I knockout mice treated with azoxymethane/dextran sulfate sodium"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Food Science & Human Nutrition"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:04Z"}