{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/127509"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/127509","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Establishing the role of vitamin a production in hepatocytes in cardiometabolic diseases","abstract":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-12-01","abstract_html":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-12-01","abstract_has_math":false,"creators":["Catalan Monroy, Walter Alexis"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Food Science & Human Nutrition","degree_department":null,"school":null,"contributors":["Amengual Terrasa, Jaume","Erdman, John W","Hasnin, Saima"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2024,"date_issued":"2024-12-12","date_published":"2024-12-12","updated_at":"2026-07-22T22:25:04Z","subjects":["Fatty Liver","Carotenoids","Cardiovascular Disease"],"languages":["en","eng"],"rights":["Copyright 2024 Walter Alexis Catalan Monroy"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/127509","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Amengual Terrasa, Jaume","Erdman, John W","Hasnin, Saima"]},{"key":"dc:creator","label":"Author","values":["Catalan Monroy, Walter Alexis"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2024-12-12","2024-12"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Food Science & Human Nutrition"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Fatty Liver","Carotenoids","Cardiovascular Disease"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2024 Walter Alexis Catalan Monroy"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/127509"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-12-01","The student, Walter Catalan Monroy, accepted the attached license on 2024-12-06 at 16:13.","The student, Walter Catalan Monroy, submitted this Thesis for approval on 2024-12-06 at 16:40.","This Thesis was approved for publication on 2024-12-12 at 08:28.","DSpace SAF Submission Ingestion Package generated from Vireo submission #21537 on 2025-03-28 at 14:57:02","The conversion of dietary β-carotene to vitamin A by the β-carotene oxygenase 1 (BCO1) mitigates cardiometabolic diseases such as atherosclerosis and liver steatosis. Followed by adipocytes, hepatocytes are the major reservoirs of β-carotene in the body. Hepatocytes also participate in vitamin A homeostasis and are key players in regulating energy metabolism. This study aims to examine the cardiometabolic effects of β-carotene in hepatocytes as a vitamin A precursor. To test the impact of hepatocyte-specific vitamin A production, we fed β-carotene to BCO1-deficient (Bco1-/-) mice and injected them with a hepatocyte-specific adeno-associated vector encoding BCO1 (L-AAV-BCO1). To study atherosclerosis, we cross-bred Bco1-/- mice with the atheroprone low-density lipoprotein receptor-deficient (Ldlr-/-) mice to generate Bco1-/-Ldlr-/- mice. We fed Bco1-/-Ldlr-/- mice a Western diet (WD) supplemented with β-carotene (WD-β-carotene) and injected them with either L-AAV-BCO1 or an L-AAV encoding green-fluorescent protein (L-AAV-GFP) as control. To study the effect of hepatocyte-specific vitamin A production in the context of liver steatosis, we fed Bco1-/- mice a high-fat, high-sucrose (HFHS) diet supplemented with β-carotene (HFHS-β-carotene). L-AAV-BCO1 infusions increased vitamin A stores and retinoic acid signaling in Bco1-/- mice fed β-carotene, mitigated atherosclerosis progression, monocytosis, and monocyte recruitment to the lesion in Bco1-/- Ldlr-/- mice fed WD with β-carotene. L-AAV-BCO1 treatment in Bco1-/- Ldlr-/- mice fed a WD-β-carotene and Bco1-/- mice fed HFHS-β-carotene resulted in an overall reduction of hepatic lipid content, increased hepatic fatty acid oxidation, and reduced lipogenesis in comparison to age, and sex-matched controls injected with L-AAV-GFP. The effects of L-AAV-BCO1 were absent in mice fed HFHS with no β-carotene, implicating vitamin A formation in the cardiometabolic effects of L-AAV-BCO1 treatments. Our findings suggest that targeting hepatic β-carotene stores in hepatocytes to produce vitamin A is a viable strategy to mitigate the development of cardiometabolic diseases."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Establishing the role of vitamin a production in hepatocytes in cardiometabolic diseases"]}]}],"canonical_facts":{"dc:contributor":["Amengual Terrasa, Jaume","Erdman, John W","Hasnin, Saima"],"dc:creator":["Catalan Monroy, Walter Alexis"],"dc:date":["2024-12-12","2024-12"],"dc:description":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-12-01","The student, Walter Catalan Monroy, accepted the attached license on 2024-12-06 at 16:13.","The student, Walter Catalan Monroy, submitted this Thesis for approval on 2024-12-06 at 16:40.","This Thesis was approved for publication on 2024-12-12 at 08:28.","DSpace SAF Submission Ingestion Package generated from Vireo submission #21537 on 2025-03-28 at 14:57:02","The conversion of dietary β-carotene to vitamin A by the β-carotene oxygenase 1 (BCO1) mitigates cardiometabolic diseases such as atherosclerosis and liver steatosis. Followed by adipocytes, hepatocytes are the major reservoirs of β-carotene in the body. Hepatocytes also participate in vitamin A homeostasis and are key players in regulating energy metabolism. This study aims to examine the cardiometabolic effects of β-carotene in hepatocytes as a vitamin A precursor. To test the impact of hepatocyte-specific vitamin A production, we fed β-carotene to BCO1-deficient (Bco1-/-) mice and injected them with a hepatocyte-specific adeno-associated vector encoding BCO1 (L-AAV-BCO1). To study atherosclerosis, we cross-bred Bco1-/- mice with the atheroprone low-density lipoprotein receptor-deficient (Ldlr-/-) mice to generate Bco1-/-Ldlr-/- mice. We fed Bco1-/-Ldlr-/- mice a Western diet (WD) supplemented with β-carotene (WD-β-carotene) and injected them with either L-AAV-BCO1 or an L-AAV encoding green-fluorescent protein (L-AAV-GFP) as control. To study the effect of hepatocyte-specific vitamin A production in the context of liver steatosis, we fed Bco1-/- mice a high-fat, high-sucrose (HFHS) diet supplemented with β-carotene (HFHS-β-carotene). L-AAV-BCO1 infusions increased vitamin A stores and retinoic acid signaling in Bco1-/- mice fed β-carotene, mitigated atherosclerosis progression, monocytosis, and monocyte recruitment to the lesion in Bco1-/- Ldlr-/- mice fed WD with β-carotene. L-AAV-BCO1 treatment in Bco1-/- Ldlr-/- mice fed a WD-β-carotene and Bco1-/- mice fed HFHS-β-carotene resulted in an overall reduction of hepatic lipid content, increased hepatic fatty acid oxidation, and reduced lipogenesis in comparison to age, and sex-matched controls injected with L-AAV-GFP. The effects of L-AAV-BCO1 were absent in mice fed HFHS with no β-carotene, implicating vitamin A formation in the cardiometabolic effects of L-AAV-BCO1 treatments. Our findings suggest that targeting hepatic β-carotene stores in hepatocytes to produce vitamin A is a viable strategy to mitigate the development of cardiometabolic diseases."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/127509"],"dc:language":["en","eng"],"dc:rights":["Copyright 2024 Walter Alexis Catalan Monroy"],"dc:subject":["Fatty Liver","Carotenoids","Cardiovascular Disease"],"dc:title":["Establishing the role of vitamin a production in hepatocytes in cardiometabolic diseases"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Food Science & Human Nutrition"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:04Z"}