{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/127434"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/127434","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Development of a synthetic biology platform to discover novel ribosomally synthesized and post-translationally modified peptides","abstract":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-12-01","abstract_html":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-12-01","abstract_has_math":false,"creators":["Shi, Chengyou"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemical Engineering","degree_department":null,"school":null,"contributors":["Zhao, Huimin","Rao, Christopher V.","Kraft, Mary L.","van der Donk, Wilfred A."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2024,"date_issued":"2024-09-03","date_published":"2024-09-03","updated_at":"2026-07-22T22:25:04Z","subjects":["Ripp","Synthetic Biology"],"languages":["en","eng"],"rights":["Copyright 2024 Chengyou Shi"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/127434","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Zhao, Huimin","Rao, Christopher V.","Kraft, Mary L.","van der Donk, Wilfred A."]},{"key":"dc:creator","label":"Author","values":["Shi, Chengyou"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2024-09-03","2024-12"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemical Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Ripp","Synthetic Biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2024 Chengyou Shi"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/127434"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-12-01","The student, Chengyou Shi, accepted the attached license on 2024-08-27 at 10:03.","The student, Chengyou Shi, submitted this Dissertation for approval on 2024-08-27 at 10:04.","This Dissertation was approved for publication on 2024-09-03 at 11:10.","DSpace SAF Submission Ingestion Package generated from Vireo submission #21204 on 2025-03-28 at 14:53:15","Natural products are invaluable in drug discovery due to their chemical diversity and intricacy, which endows them with diverse mechanisms of action. The discovery of new natural products will benefit identifying new drug leads and advancing pharmaceutical research. Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a unique group of peptide natural products and are a new source of antimicrobials in the face of the antimicrobial resistance crisis. The methods for RiPPs discovery have undergone a revolution due to the explosion of available genomic data and the development of advanced bioinformatics tools. Recent bioinformatics studies have revealed a vast number of prokaryotic RiPP biosynthetic gene clusters (BGCs) predicted to encode new molecular scaffolds and potentially useful biological functions. However, the main challenge in accessing this vast number of uncharacterized RiPPs is the lack of tools to efficiently discover and characterize the BGCs that originate from diverse bacterial phyla. The overall goal of my Ph.D. research is to develop synthetic biology strategies to address the challenges associated with the discovery and characterization of RiPP BGCs for drug development and discovery."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Development of a synthetic biology platform to discover novel ribosomally synthesized and post-translationally modified peptides"]}]}],"canonical_facts":{"dc:contributor":["Zhao, Huimin","Rao, Christopher V.","Kraft, Mary L.","van der Donk, Wilfred A."],"dc:creator":["Shi, Chengyou"],"dc:date":["2024-09-03","2024-12"],"dc:description":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-12-01","The student, Chengyou Shi, accepted the attached license on 2024-08-27 at 10:03.","The student, Chengyou Shi, submitted this Dissertation for approval on 2024-08-27 at 10:04.","This Dissertation was approved for publication on 2024-09-03 at 11:10.","DSpace SAF Submission Ingestion Package generated from Vireo submission #21204 on 2025-03-28 at 14:53:15","Natural products are invaluable in drug discovery due to their chemical diversity and intricacy, which endows them with diverse mechanisms of action. The discovery of new natural products will benefit identifying new drug leads and advancing pharmaceutical research. Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a unique group of peptide natural products and are a new source of antimicrobials in the face of the antimicrobial resistance crisis. The methods for RiPPs discovery have undergone a revolution due to the explosion of available genomic data and the development of advanced bioinformatics tools. Recent bioinformatics studies have revealed a vast number of prokaryotic RiPP biosynthetic gene clusters (BGCs) predicted to encode new molecular scaffolds and potentially useful biological functions. However, the main challenge in accessing this vast number of uncharacterized RiPPs is the lack of tools to efficiently discover and characterize the BGCs that originate from diverse bacterial phyla. The overall goal of my Ph.D. research is to develop synthetic biology strategies to address the challenges associated with the discovery and characterization of RiPP BGCs for drug development and discovery."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/127434"],"dc:language":["en","eng"],"dc:rights":["Copyright 2024 Chengyou Shi"],"dc:subject":["Ripp","Synthetic Biology"],"dc:title":["Development of a synthetic biology platform to discover novel ribosomally synthesized and post-translationally modified peptides"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Chemical Engineering"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:04Z"}