{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/127390"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/127390","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Label-free polarization-sensitive multimodal optical microscopy for collagen characterization in tissue","abstract":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2026-12-01","abstract_html":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2026-12-01","abstract_has_math":false,"creators":["Yang, Lingxiao"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Electrical & Computer Engr","degree_department":null,"school":null,"contributors":["Boppart, Stephen A","Bhargava, Rohit","Dragic, Peter D","Zhao, Yang"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2024,"date_issued":"2024-12-06","date_published":"2024-12-06","updated_at":"2026-07-22T22:25:04Z","subjects":["Optical Imaging","Biomedical Optics","Collagen"],"languages":["eng","en"],"rights":["Copyright 2024 Lingxiao Yang"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/127390","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Boppart, Stephen A","Bhargava, Rohit","Dragic, Peter D","Zhao, Yang"]},{"key":"dc:creator","label":"Author","values":["Yang, Lingxiao"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2024-12-06","2024-12"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Electrical & Computer Engr"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Optical Imaging","Biomedical Optics","Collagen"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng","en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2024 Lingxiao Yang"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/127390"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2026-12-01","The student, Lingxiao Yang, accepted the attached license on 2024-12-06 at 11:45.","The student, Lingxiao Yang, submitted this Dissertation for approval on 2024-12-06 at 13:16.","This Dissertation was approved for publication on 2024-12-06 at 15:10.","DSpace SAF Submission Ingestion Package generated from Vireo submission #21476 on 2025-03-28 at 14:44:33","This thesis presents the development and application of a novel multimodal polarization-sensitive optical microscopy system for comprehensive characterization of collagen in biological tissues, with a particular focus on tumor microenvironments. The work addresses the critical need for advanced imaging techniques to study collagen beyond morphological features, offering unprecedented insights into its structural, biophysical and biochemical properties across multiple scales. The thesis first introduces the important role of collagen in the extracellular matrix and tumor progression, and the concept of tumor-associated collagen signatures (TACS). Details on the methodology of the custom-built multimodal imaging system are then described, integrating polarization-sensitive second harmonic generation (PSHG), polarization-sensitive optical coherence microscopy (PSOCM), two-photon autofluorescence lifetime imaging microscopy (2PFLIM), and coherent anti-Stokes Raman scattering (CARS) microscopy. Key technical innovations include the design and calibration of a custom Fourier transform pulse shaper for low-photodamage CARS are highlighted. The system's capabilities are first demonstrated through the characterization of different collagen types (I-IV) in purified gels and complex tissue environments. Distinct optical signatures are identified for each collagen type, and the integration of PSOCM measurements into the PSHG numerical model significantly improves collagen helix angle estimations in thick tissues. A comprehensive analysis of collagen organization in rodent outer ears reveals depth-dependent distributions of different collagen types and enables identification of complex structures."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Label-free polarization-sensitive multimodal optical microscopy for collagen characterization in tissue"]}]}],"canonical_facts":{"dc:contributor":["Boppart, Stephen A","Bhargava, Rohit","Dragic, Peter D","Zhao, Yang"],"dc:creator":["Yang, Lingxiao"],"dc:date":["2024-12-06","2024-12"],"dc:description":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2026-12-01","The student, Lingxiao Yang, accepted the attached license on 2024-12-06 at 11:45.","The student, Lingxiao Yang, submitted this Dissertation for approval on 2024-12-06 at 13:16.","This Dissertation was approved for publication on 2024-12-06 at 15:10.","DSpace SAF Submission Ingestion Package generated from Vireo submission #21476 on 2025-03-28 at 14:44:33","This thesis presents the development and application of a novel multimodal polarization-sensitive optical microscopy system for comprehensive characterization of collagen in biological tissues, with a particular focus on tumor microenvironments. The work addresses the critical need for advanced imaging techniques to study collagen beyond morphological features, offering unprecedented insights into its structural, biophysical and biochemical properties across multiple scales. The thesis first introduces the important role of collagen in the extracellular matrix and tumor progression, and the concept of tumor-associated collagen signatures (TACS). Details on the methodology of the custom-built multimodal imaging system are then described, integrating polarization-sensitive second harmonic generation (PSHG), polarization-sensitive optical coherence microscopy (PSOCM), two-photon autofluorescence lifetime imaging microscopy (2PFLIM), and coherent anti-Stokes Raman scattering (CARS) microscopy. Key technical innovations include the design and calibration of a custom Fourier transform pulse shaper for low-photodamage CARS are highlighted. The system's capabilities are first demonstrated through the characterization of different collagen types (I-IV) in purified gels and complex tissue environments. Distinct optical signatures are identified for each collagen type, and the integration of PSOCM measurements into the PSHG numerical model significantly improves collagen helix angle estimations in thick tissues. A comprehensive analysis of collagen organization in rodent outer ears reveals depth-dependent distributions of different collagen types and enables identification of complex structures."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/127390"],"dc:language":["eng","en"],"dc:rights":["Copyright 2024 Lingxiao Yang"],"dc:subject":["Optical Imaging","Biomedical Optics","Collagen"],"dc:title":["Label-free polarization-sensitive multimodal optical microscopy for collagen characterization in tissue"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Electrical & Computer Engr"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:04Z"}