{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/125824"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/125824","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Ice control by antifreeze proteins: From interfacial thermodynamics, and engulfment resistance, to thermal hysteresis activity using theory and computation","abstract":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-08-01","abstract_html":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-08-01","abstract_has_math":false,"creators":["Farag, Hossam"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Nuclear, Plasma, Radiolgc Engr","degree_department":null,"school":null,"contributors":["Peters, Baron","Stubbins, James F","Schroeder, Charles","Xi, Jianqi"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2024,"date_issued":"2024-07-12","date_published":"2024-07-12","updated_at":"2026-07-22T22:25:02Z","subjects":["Antifreeze Proteins","Nucleation","Ice Growth","Metastable Liquids","Thermal Hysteresis","Growth Inhibitors"],"languages":["en","eng"],"rights":["Copyright 2024 Hossam Farag"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/125824","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Peters, Baron","Stubbins, James F","Schroeder, Charles","Xi, Jianqi"]},{"key":"dc:creator","label":"Author","values":["Farag, Hossam"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2024-07-12","2024-08"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Nuclear, Plasma, Radiolgc Engr"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Antifreeze Proteins","Nucleation","Ice Growth","Metastable Liquids","Thermal Hysteresis","Growth Inhibitors"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2024 Hossam Farag"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/125824"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-08-01","The student, Hossam Farag, accepted the attached license on 2024-07-11 at 14:26.","The student, Hossam Farag, submitted this Dissertation for approval on 2024-07-11 at 14:33.","This Dissertation was approved for publication on 2024-07-12 at 11:11.","DSpace SAF Submission Ingestion Package generated from Vireo submission #21074 on 2025-02-04 at 21:25:52","Mitigating ice damage is crucial in various applications, including aircrafts and nuclear power plants, where ice formation in feed water systems can cause significant operational challenges. Antifreeze proteins (AFPs) play a vital role in enabling organisms to survive freezing temperatures by binding to ice surfaces and inhibiting ice growth. Ice growth inhibition can be sustained until a certain limit of supercooling temperature, below which ice irreversibly overgrows the pinning AFPs. The difference between the ordinary freezing temperature of water (0 °C) and the lowered freezing temperature caused by AFPs is termed thermal hysteresis (TH) activity. Mechanistically, TH is first achieved through the adsorption of AFPs on ice crystals. Each adsorbed AFP creates a nanometer-scale metastable depression on the ice surface, locally resisting ice growth. As supercooling increases, these metastable dimples deepen until metastability is lost in an engulfment event where the ice irreversibly swallows the AFP. This engulfment process is akin to nucleation, and we develop a theoretical model using variational optimization to estimate the free energy barrier for the engulfment of a single AFP as a function of supercooling, AFP size, and isolation radius from neighboring AFPs. The results of the model are summarized in a symbolic regression closed-form expression. On a larger length scale, where an ensemble of AFPs is adsorbed to a micrometer-scale ice surface, the collective resistance to engulfment is estimated through a Voronoi tessellation of the surface. This approach maps the 2D configurational distribution of AFPs to a distribution of isolation radii from neighboring AFPs. The isolation radii distribution is used to statistically weigh individual AFP engulfment rates, resulting in an estimation of the collective engulfment rate of the entire ensemble of adsorbed AFPs. Kinetic Monte Carlo simulations have shown that the first engulfment event can trigger an avalanche of subsequent events, leading to unrestrained ice growth. Hence, TH activity can be estimated to be the temperature at which the first engulfment event occurs. This temperature is estimated using an inhomogeneous survival probability model that accounts for AFP surface coverage, ice surface area, and cooling rate. Theoretically estimated TH values were compared with experimental data and showed agreement. Lastly, the developed framework was used to rationalize how the TH activity of irreversibly adsorbed AFPs on the ice surface can be dependent on the AFPs' bulk concentration in the solution interfacing the restrained ice crystal. Our model predicts a collapse of different bulk concentrations and exposure times, resulting in similar AFP surface coverage to a single TH value. Re-analysis of literature experimental data confirmed this collapse, verifying the recently developed model's accuracy. Our model is the first in the literature to predict the sudden burst of ice growth exactly as observed in experiments. The findings provide a comprehensive understanding of the factors influencing AFP effectiveness, align well with experimental observations, and offer insights into the design of synthetic antifreeze agents for practical applications."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Ice control by antifreeze proteins: From interfacial thermodynamics, and engulfment resistance, to thermal hysteresis activity using theory and computation"]}]}],"canonical_facts":{"dc:contributor":["Peters, Baron","Stubbins, James F","Schroeder, Charles","Xi, Jianqi"],"dc:creator":["Farag, Hossam"],"dc:date":["2024-07-12","2024-08"],"dc:description":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-08-01","The student, Hossam Farag, accepted the attached license on 2024-07-11 at 14:26.","The student, Hossam Farag, submitted this Dissertation for approval on 2024-07-11 at 14:33.","This Dissertation was approved for publication on 2024-07-12 at 11:11.","DSpace SAF Submission Ingestion Package generated from Vireo submission #21074 on 2025-02-04 at 21:25:52","Mitigating ice damage is crucial in various applications, including aircrafts and nuclear power plants, where ice formation in feed water systems can cause significant operational challenges. Antifreeze proteins (AFPs) play a vital role in enabling organisms to survive freezing temperatures by binding to ice surfaces and inhibiting ice growth. Ice growth inhibition can be sustained until a certain limit of supercooling temperature, below which ice irreversibly overgrows the pinning AFPs. The difference between the ordinary freezing temperature of water (0 °C) and the lowered freezing temperature caused by AFPs is termed thermal hysteresis (TH) activity. Mechanistically, TH is first achieved through the adsorption of AFPs on ice crystals. Each adsorbed AFP creates a nanometer-scale metastable depression on the ice surface, locally resisting ice growth. As supercooling increases, these metastable dimples deepen until metastability is lost in an engulfment event where the ice irreversibly swallows the AFP. This engulfment process is akin to nucleation, and we develop a theoretical model using variational optimization to estimate the free energy barrier for the engulfment of a single AFP as a function of supercooling, AFP size, and isolation radius from neighboring AFPs. The results of the model are summarized in a symbolic regression closed-form expression. On a larger length scale, where an ensemble of AFPs is adsorbed to a micrometer-scale ice surface, the collective resistance to engulfment is estimated through a Voronoi tessellation of the surface. This approach maps the 2D configurational distribution of AFPs to a distribution of isolation radii from neighboring AFPs. The isolation radii distribution is used to statistically weigh individual AFP engulfment rates, resulting in an estimation of the collective engulfment rate of the entire ensemble of adsorbed AFPs. Kinetic Monte Carlo simulations have shown that the first engulfment event can trigger an avalanche of subsequent events, leading to unrestrained ice growth. Hence, TH activity can be estimated to be the temperature at which the first engulfment event occurs. This temperature is estimated using an inhomogeneous survival probability model that accounts for AFP surface coverage, ice surface area, and cooling rate. Theoretically estimated TH values were compared with experimental data and showed agreement. Lastly, the developed framework was used to rationalize how the TH activity of irreversibly adsorbed AFPs on the ice surface can be dependent on the AFPs' bulk concentration in the solution interfacing the restrained ice crystal. Our model predicts a collapse of different bulk concentrations and exposure times, resulting in similar AFP surface coverage to a single TH value. Re-analysis of literature experimental data confirmed this collapse, verifying the recently developed model's accuracy. Our model is the first in the literature to predict the sudden burst of ice growth exactly as observed in experiments. The findings provide a comprehensive understanding of the factors influencing AFP effectiveness, align well with experimental observations, and offer insights into the design of synthetic antifreeze agents for practical applications."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/125824"],"dc:language":["en","eng"],"dc:rights":["Copyright 2024 Hossam Farag"],"dc:subject":["Antifreeze Proteins","Nucleation","Ice Growth","Metastable Liquids","Thermal Hysteresis","Growth Inhibitors"],"dc:title":["Ice control by antifreeze proteins: From interfacial thermodynamics, and engulfment resistance, to thermal hysteresis activity using theory and computation"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Nuclear, Plasma, Radiolgc Engr"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:02Z"}