{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/125657"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/125657","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Development of novel light-based molecular tools for biomedical applications","abstract":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2026-08-01","abstract_html":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2026-08-01","abstract_has_math":false,"creators":["Tapia Hernandez, Rodrigo"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Chan, Jefferson K","Manesis, Anastasia C","Murphy, Catherine J","Sweedler, Jonathan V"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2024,"date_issued":"2024-05-16","date_published":"2024-05-16","updated_at":"2026-07-22T22:25:02Z","subjects":["Molecular Tools","Biomedical Applications","Cargo Delivery","Light-based Therapeutics"],"languages":["en","eng"],"rights":["Copyright 2024, Rodrigo Tapia Hernandez"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/125657","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Chan, Jefferson K","Manesis, Anastasia C","Murphy, Catherine J","Sweedler, Jonathan V"]},{"key":"dc:creator","label":"Author","values":["Tapia Hernandez, Rodrigo"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2024-05-16","2024-08"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Molecular Tools","Biomedical Applications","Cargo Delivery","Light-based Therapeutics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2024, Rodrigo Tapia Hernandez"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/125657"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2026-08-01","The student, Rodrigo Tapia Hernandez, accepted the attached license on 2024-05-08 at 10:52.","The student, Rodrigo Tapia Hernandez, submitted this Dissertation for approval on 2024-05-08 at 11:02.","This Dissertation was approved for publication on 2024-05-16 at 14:59.","DSpace SAF Submission Ingestion Package generated from Vireo submission #20783 on 2025-02-04 at 21:15:31","Light is an important tool that can be applied in a wide range of chemical and biological applications. Light can provide both spatial and temporal control over chemical processes that we can exploit to help us better understand the chemical processes in biological systems. This can be achieved through the use of targetable colorimetric and fluorescent probes, as well as light-mediated chemical reactions in photolabile molecules, which can be used to deliver analytes, probes, and even therapeutics in a localized region of interest. In addition, light has the advantage of being a non-invasive trigger, and by tunning the power and wavelength it can be used safely without causing any damage or distress to healthy cells or tissues. The development of these tools has played an important role in biomedical applications, for example: basic studies to understand biological processes; disease monitoring through molecular imaging; direct light-mediated therapy with photothermal therapy, photodynamic therapy, and activatable drug release. In this work, Chapter 1 will give an introduction on the importance of light as a tool in both chemical and biological processes. In addition, examples of how modifications to dyes can be exploited for various imaging techniques, including a modern imaging modality known as photoacoustic imaging. Chapter 2 will expand on the photoacoustic effect, which takes advantage of the imaging depth that ultrasound imaging provides with the specificity of light as the excitation source. This chapter also presents a method of introducing this imaging modality to laboratories without expensive instrumentation. In Chapter 3, a novel photodegradable nano-hydrogel will be introduced for the delivery of multiple types of cargo in cells and in vivo. The ability to use NIR-light to improve the spatiotemporal control of release of a chemotherapeutic can be of great advantage to improve both the efficacy of the drug and its biosafety by reducing off-target interactions. Appendix C also presents the development of an enzyme mediated delivery of methylene blue, a popular molecule that can be used as a photosensitizer as a therapeutic to treat cancer, and specifically target M1 polarized macrophages which are known to be present in the tumor microenvironment."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Development of novel light-based molecular tools for biomedical applications"]}]}],"canonical_facts":{"dc:contributor":["Chan, Jefferson K","Manesis, Anastasia C","Murphy, Catherine J","Sweedler, Jonathan V"],"dc:creator":["Tapia Hernandez, Rodrigo"],"dc:date":["2024-05-16","2024-08"],"dc:description":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2026-08-01","The student, Rodrigo Tapia Hernandez, accepted the attached license on 2024-05-08 at 10:52.","The student, Rodrigo Tapia Hernandez, submitted this Dissertation for approval on 2024-05-08 at 11:02.","This Dissertation was approved for publication on 2024-05-16 at 14:59.","DSpace SAF Submission Ingestion Package generated from Vireo submission #20783 on 2025-02-04 at 21:15:31","Light is an important tool that can be applied in a wide range of chemical and biological applications. Light can provide both spatial and temporal control over chemical processes that we can exploit to help us better understand the chemical processes in biological systems. This can be achieved through the use of targetable colorimetric and fluorescent probes, as well as light-mediated chemical reactions in photolabile molecules, which can be used to deliver analytes, probes, and even therapeutics in a localized region of interest. In addition, light has the advantage of being a non-invasive trigger, and by tunning the power and wavelength it can be used safely without causing any damage or distress to healthy cells or tissues. The development of these tools has played an important role in biomedical applications, for example: basic studies to understand biological processes; disease monitoring through molecular imaging; direct light-mediated therapy with photothermal therapy, photodynamic therapy, and activatable drug release. In this work, Chapter 1 will give an introduction on the importance of light as a tool in both chemical and biological processes. In addition, examples of how modifications to dyes can be exploited for various imaging techniques, including a modern imaging modality known as photoacoustic imaging. Chapter 2 will expand on the photoacoustic effect, which takes advantage of the imaging depth that ultrasound imaging provides with the specificity of light as the excitation source. This chapter also presents a method of introducing this imaging modality to laboratories without expensive instrumentation. In Chapter 3, a novel photodegradable nano-hydrogel will be introduced for the delivery of multiple types of cargo in cells and in vivo. The ability to use NIR-light to improve the spatiotemporal control of release of a chemotherapeutic can be of great advantage to improve both the efficacy of the drug and its biosafety by reducing off-target interactions. Appendix C also presents the development of an enzyme mediated delivery of methylene blue, a popular molecule that can be used as a photosensitizer as a therapeutic to treat cancer, and specifically target M1 polarized macrophages which are known to be present in the tumor microenvironment."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/125657"],"dc:language":["en","eng"],"dc:rights":["Copyright 2024, Rodrigo Tapia Hernandez"],"dc:subject":["Molecular Tools","Biomedical Applications","Cargo Delivery","Light-based Therapeutics"],"dc:title":["Development of novel light-based molecular tools for biomedical applications"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:02Z"}