{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/124726"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/124726","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Enhance heme biosynthesis in Saccharomyces cerevisiae","abstract":"This research delves into the enhancement of heme production in Saccharomyces cerevisiae through Adaptive Laboratory Evolution (ALE) and Atmospheric and Room Temperature Plasma (ARTP) mutagenesis, complemented by strategic nutrient supplementation. Despite the theoretical promise of ALE and ARTP to induce beneficial mutations for augmented heme biosynthesis, the results highlighted the difficulties in modifying metabolic pathways for intended enhancements. Additionally, the supplementation of ferric ions and glycine, aimed at increasing the availability of precursors for heme biosynthesis, did not yield a significant increase in heme production, as evidenced by quantitative measurements of heme and its direct precursor, protoporphyrin IX (PpIX). The challenges encountered in the high-throughput screening for mutants with enhanced heme production underscored the need for more refined detection methods to identify and quantify metabolic improvements accurately. This research contributes to the understanding of the limitations and potentials of using evolutionary and mutagenesis techniques in yeast strain development, emphasizing the need for an integrated approach that combines genetic, biochemical, and analytical strategies to advance microbial production of valuable metabolites.","abstract_html":"This research delves into the enhancement of heme production in Saccharomyces cerevisiae through Adaptive Laboratory Evolution (ALE) and Atmospheric and Room Temperature Plasma (ARTP) mutagenesis, complemented by strategic nutrient supplementation. Despite the theoretical promise of ALE and ARTP to induce beneficial mutations for augmented heme biosynthesis, the results highlighted the difficulties in modifying metabolic pathways for intended enhancements. Additionally, the supplementation of ferric ions and glycine, aimed at increasing the availability of precursors for heme biosynthesis, did not yield a significant increase in heme production, as evidenced by quantitative measurements of heme and its direct precursor, protoporphyrin IX (PpIX). The challenges encountered in the high-throughput screening for mutants with enhanced heme production underscored the need for more refined detection methods to identify and quantify metabolic improvements accurately. This research contributes to the understanding of the limitations and potentials of using evolutionary and mutagenesis techniques in yeast strain development, emphasizing the need for an integrated approach that combines genetic, biochemical, and analytical strategies to advance microbial production of valuable metabolites.","abstract_has_math":false,"creators":["Deng, Lingchi"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Food Science & Human Nutrition","degree_department":null,"school":null,"contributors":["Jin, Yong-su","Miller, Michael","Chen, Hong"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2024,"date_issued":"2024-05","date_published":"2024-05","updated_at":"2026-07-22T22:25:02Z","subjects":["Precision Fermentation","Heme Biosynthesis"],"languages":["eng","en"],"rights":["Copyright 2024 Lingchi Deng"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/124726","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Jin, Yong-su","Miller, Michael","Chen, Hong"]},{"key":"dc:creator","label":"Author","values":["Deng, Lingchi"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2024-05","2024-05-03"]},{"key":"dc:type","label":"Dc Type","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Food Science & Human Nutrition"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Precision Fermentation","Heme Biosynthesis"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng","en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2024 Lingchi Deng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/124726"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["This research delves into the enhancement of heme production in Saccharomyces cerevisiae through Adaptive Laboratory Evolution (ALE) and Atmospheric and Room Temperature Plasma (ARTP) mutagenesis, complemented by strategic nutrient supplementation. Despite the theoretical promise of ALE and ARTP to induce beneficial mutations for augmented heme biosynthesis, the results highlighted the difficulties in modifying metabolic pathways for intended enhancements. Additionally, the supplementation of ferric ions and glycine, aimed at increasing the availability of precursors for heme biosynthesis, did not yield a significant increase in heme production, as evidenced by quantitative measurements of heme and its direct precursor, protoporphyrin IX (PpIX). The challenges encountered in the high-throughput screening for mutants with enhanced heme production underscored the need for more refined detection methods to identify and quantify metabolic improvements accurately. This research contributes to the understanding of the limitations and potentials of using evolutionary and mutagenesis techniques in yeast strain development, emphasizing the need for an integrated approach that combines genetic, biochemical, and analytical strategies to advance microbial production of valuable metabolites.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-05-01","The student, Lingchi Deng, accepted the attached license on 2024-05-02 at 18:39.","The student, Lingchi Deng, submitted this Thesis for approval on 2024-05-02 at 19:22.","This Thesis was approved for publication on 2024-05-03 at 13:48.","DSpace SAF Submission Ingestion Package generated from Vireo submission #20768 on 2024-09-16 at 00:51:15"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Enhance heme biosynthesis in Saccharomyces cerevisiae"]}]}],"canonical_facts":{"dc:contributor":["Jin, Yong-su","Miller, Michael","Chen, Hong"],"dc:creator":["Deng, Lingchi"],"dc:date":["2024-05","2024-05-03"],"dc:description":["This research delves into the enhancement of heme production in Saccharomyces cerevisiae through Adaptive Laboratory Evolution (ALE) and Atmospheric and Room Temperature Plasma (ARTP) mutagenesis, complemented by strategic nutrient supplementation. Despite the theoretical promise of ALE and ARTP to induce beneficial mutations for augmented heme biosynthesis, the results highlighted the difficulties in modifying metabolic pathways for intended enhancements. Additionally, the supplementation of ferric ions and glycine, aimed at increasing the availability of precursors for heme biosynthesis, did not yield a significant increase in heme production, as evidenced by quantitative measurements of heme and its direct precursor, protoporphyrin IX (PpIX). The challenges encountered in the high-throughput screening for mutants with enhanced heme production underscored the need for more refined detection methods to identify and quantify metabolic improvements accurately. This research contributes to the understanding of the limitations and potentials of using evolutionary and mutagenesis techniques in yeast strain development, emphasizing the need for an integrated approach that combines genetic, biochemical, and analytical strategies to advance microbial production of valuable metabolites.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-05-01","The student, Lingchi Deng, accepted the attached license on 2024-05-02 at 18:39.","The student, Lingchi Deng, submitted this Thesis for approval on 2024-05-02 at 19:22.","This Thesis was approved for publication on 2024-05-03 at 13:48.","DSpace SAF Submission Ingestion Package generated from Vireo submission #20768 on 2024-09-16 at 00:51:15"],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/124726"],"dc:language":["eng","en"],"dc:rights":["Copyright 2024 Lingchi Deng"],"dc:subject":["Precision Fermentation","Heme Biosynthesis"],"dc:title":["Enhance heme biosynthesis in Saccharomyces cerevisiae"],"dc:type":["Text"],"thesis:degree_discipline":["Food Science & Human Nutrition"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:02Z"}