{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/124487"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/124487","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Label-free optical imaging of neural activity","abstract":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2026-05-01","abstract_html":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2026-05-01","abstract_has_math":false,"creators":["Iyer, Rishyashring Raman"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Electrical and Computer Engineering","degree_department":null,"school":null,"contributors":["Boppart, Stephen A","Gillette, Martha U","Vlasov, Yurii","Chen, Yun-Sheng"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2024,"date_issued":"2024-05","date_published":"2024-05","updated_at":"2026-07-22T22:25:02Z","subjects":["Optical Imaging","Biophotonics","Neuroimaging","Microscopy","Neurophotonics","Multiphoton Microscopy","Optical Coherence Tomography","Biomedical Imaging"],"languages":["en","eng"],"rights":["Copyright 2024 Rishyashring Iyer"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/124487","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Boppart, Stephen A","Gillette, Martha U","Vlasov, Yurii","Chen, Yun-Sheng"]},{"key":"dc:creator","label":"Author","values":["Iyer, Rishyashring Raman"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2024-05","2024-02-16"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Electrical and Computer Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Optical Imaging","Biophotonics","Neuroimaging","Microscopy","Neurophotonics","Multiphoton Microscopy","Optical Coherence Tomography","Biomedical Imaging"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2024 Rishyashring Iyer"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/124487"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2026-05-01","The student, Rishyashring Iyer, accepted the attached license on 2024-02-13 at 16:55.","The student, Rishyashring Iyer, submitted this Dissertation for approval on 2024-02-13 at 18:04.","This Dissertation was approved for publication on 2024-02-16 at 16:44.","DSpace SAF Submission Ingestion Package generated from Vireo submission #20219 on 2024-09-16 at 00:42:04","The progress in laser physics has propelled optical microscopy to the forefront of biological and biomedical imaging, spanning from fundamental biology to clinical diagnosis. Traditional microscopes necessitate external contrast agents, impacting cell environments. However, advanced label-free techniques, such as optical interferometry, autofluorescence lifetime imaging, and vibrational spectroscopy, cleverly exploit light-matter interactions, enabling live sample imaging without external labels. There is a current need to develop these techniques to target specific physiological processes rather than a generalized observation of dynamics. Particularly, the technologies to examine the neuronal microenvironment label-free remain critically underexplored. There is a gap in our knowledge of underlying metabolic, biochemical, and electrophysiological mechanisms behind several neurological processes at a cellular level. This gap can be traced back to the lack of versatile and high-throughput tools to investigate neural networks functionally. This thesis centers on developing label-free optical tools for real-time observation of native-state neuronal activity. In this thesis, four contrasts were explored as mechanisms to study neuronal activity, namely optical path length and scattering, birefringence, autofluorescence from metabolic cofactors and molecules, and local biochemistry. Overcoming challenges of observing neuronal activity spanning three orders of magnitude in space and time, new microscopes had to be developed to capture these contrasts quickly, with high resolution, over a large field of view, and multimodally. Towards this, two microscopes were developed, called Superfast Polarization-sensitive Off-axis Full-field (SPoOF) Optical Coherence Microscopy (OCM) and Versatile Autofluorescence lifetime, Multiharmonic generation, Polarization-sensitive Interferometry, and Raman imaging in Epi-detection (VAMPIRE) microscopy. The former could capture the optical phase and birefringence at 4000 frames per second over a large field of view with nanometer-scale displacement sensitivity. The latter combines autofluorescence lifetime imaging, coherent Raman scattering, and phase and polarization-sensitive OCM into a single-source microscope. The doctoral thesis details the development and application of these setups, showcasing their effectiveness in studying the neuronal microenvironment in vitro and ex vivo. SPoOF OCM captures network activity at a millisecond scale with cellular resolution, while VAMPIRE microscopy simultaneously observes multiple aspects of neuronal dynamics in 3D and real-time. These tools mark a transition from outdated methods to advanced, high-throughput optophysiology, providing valuable insights into complex neuronal processes."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Label-free optical imaging of neural activity"]}]}],"canonical_facts":{"dc:contributor":["Boppart, Stephen A","Gillette, Martha U","Vlasov, Yurii","Chen, Yun-Sheng"],"dc:creator":["Iyer, Rishyashring Raman"],"dc:date":["2024-05","2024-02-16"],"dc:description":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2026-05-01","The student, Rishyashring Iyer, accepted the attached license on 2024-02-13 at 16:55.","The student, Rishyashring Iyer, submitted this Dissertation for approval on 2024-02-13 at 18:04.","This Dissertation was approved for publication on 2024-02-16 at 16:44.","DSpace SAF Submission Ingestion Package generated from Vireo submission #20219 on 2024-09-16 at 00:42:04","The progress in laser physics has propelled optical microscopy to the forefront of biological and biomedical imaging, spanning from fundamental biology to clinical diagnosis. Traditional microscopes necessitate external contrast agents, impacting cell environments. However, advanced label-free techniques, such as optical interferometry, autofluorescence lifetime imaging, and vibrational spectroscopy, cleverly exploit light-matter interactions, enabling live sample imaging without external labels. There is a current need to develop these techniques to target specific physiological processes rather than a generalized observation of dynamics. Particularly, the technologies to examine the neuronal microenvironment label-free remain critically underexplored. There is a gap in our knowledge of underlying metabolic, biochemical, and electrophysiological mechanisms behind several neurological processes at a cellular level. This gap can be traced back to the lack of versatile and high-throughput tools to investigate neural networks functionally. This thesis centers on developing label-free optical tools for real-time observation of native-state neuronal activity. In this thesis, four contrasts were explored as mechanisms to study neuronal activity, namely optical path length and scattering, birefringence, autofluorescence from metabolic cofactors and molecules, and local biochemistry. Overcoming challenges of observing neuronal activity spanning three orders of magnitude in space and time, new microscopes had to be developed to capture these contrasts quickly, with high resolution, over a large field of view, and multimodally. Towards this, two microscopes were developed, called Superfast Polarization-sensitive Off-axis Full-field (SPoOF) Optical Coherence Microscopy (OCM) and Versatile Autofluorescence lifetime, Multiharmonic generation, Polarization-sensitive Interferometry, and Raman imaging in Epi-detection (VAMPIRE) microscopy. The former could capture the optical phase and birefringence at 4000 frames per second over a large field of view with nanometer-scale displacement sensitivity. The latter combines autofluorescence lifetime imaging, coherent Raman scattering, and phase and polarization-sensitive OCM into a single-source microscope. The doctoral thesis details the development and application of these setups, showcasing their effectiveness in studying the neuronal microenvironment in vitro and ex vivo. SPoOF OCM captures network activity at a millisecond scale with cellular resolution, while VAMPIRE microscopy simultaneously observes multiple aspects of neuronal dynamics in 3D and real-time. These tools mark a transition from outdated methods to advanced, high-throughput optophysiology, providing valuable insights into complex neuronal processes."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/124487"],"dc:language":["en","eng"],"dc:rights":["Copyright 2024 Rishyashring Iyer"],"dc:subject":["Optical Imaging","Biophotonics","Neuroimaging","Microscopy","Neurophotonics","Multiphoton Microscopy","Optical Coherence Tomography","Biomedical Imaging"],"dc:title":["Label-free optical imaging of neural activity"],"dc:type":["text"],"thesis:degree_discipline":["Electrical and Computer Engineering"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:02Z"}