{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/124364"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/124364","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Sialoadhesin is not required for infection of pigs with PRRSV-1","abstract":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-09-16 without embargo terms","abstract_html":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-09-16 without embargo terms","abstract_has_math":false,"creators":["Salgado, Brianna Crystine"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"VMS - Pathobiology","degree_department":null,"school":null,"contributors":["Rowland, Raymond R","Love, James F","Fang, Ying"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2024,"date_issued":"2024-05","date_published":"2024-05","updated_at":"2026-07-22T22:25:00Z","subjects":["Prrsv-1 Sialoadhesin Cd169 Sn"],"languages":["en","eng"],"rights":["Copyright 2024 Brianna Salgado"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/124364","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Rowland, Raymond R","Love, James F","Fang, Ying"]},{"key":"dc:creator","label":"Author","values":["Salgado, Brianna Crystine"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2024-05","2024-04-29"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["VMS - Pathobiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Prrsv-1 Sialoadhesin Cd169 Sn"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2024 Brianna Salgado"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/124364"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-09-16 without embargo terms","The student, Brianna Salgado, accepted the attached license on 2024-04-22 at 13:42.","The student, Brianna Salgado, submitted this Thesis for approval on 2024-04-22 at 13:53.","This Thesis was approved for publication on 2024-04-29 at 12:54.","DSpace SAF Submission Ingestion Package generated from Vireo submission #20544 on 2024-09-16 at 00:35:47","Sialoadhesin (Sn/SIGLEC1/CD169) is a macrophage surface protein belonging to a family of sialic acid binding immunoglobulin-like lectins. Classic models of PRRSV infection show that the first step in the infection of a macrophage is the binding of CD169 with the virion surface protein GP5. Previously, our lab discovered that CD169 knockout (KO) pigs support productive infection with PRRSV-2. Since PRRSV-1 and PRRSV-2 share only about 70% nucleotide identity and possess different requirements for infection of macrophages, we conducted a second study to determine if CD169 KO pigs could be infected with a PRRSV-1 isolate. KO pigs (n=8) and wild type pigs (WT, n=9) were infected with EU PRRSV-1 strain, SD01-08, and maintained for 28 days. Both genotypes were maintained in the same room and personnel were blind as to the genotype of individual pigs. Serum and tissues were collected and evaluated for infection using a commercial RT-qPCR kit. Antibody was measured using a commercial ELISA kit from IDEXX. Lung, tonsil, and submandibular lymph node tissue were extracted on day 28 for evaluation of viral RNA. Results from Serum and ELISA assays showed that all pigs in the wild-type (WT) and KO pigs supported PRRSV infection. Significant differences were observed in viral nucleic acid between WT and KO pigs at 21 (p = 0.002) and 28 (p = 0.023) days after infection. ELISA antibody analysis revealed statistically significant differences at 28 days post infection (p = 0 .021). Area under the curve analysis of viremia showed that the CD169 KO pigs supported higher virus loads. Analysis of tissues revealed no statistical differences between KO and WT pigs in tonsil and lymph node, while virus nucleic acid template copies were significantly higher in lung tissue from KO pigs. These results confirm that the presence of CD169 is not biologically relevant for PRRSV infection. Increased levels of viremia in KO pigs may be related to immune modulation by CD169, given the receptor is responsible for cell-to-cell interactions during antigen processing and presentation."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Sialoadhesin is not required for infection of pigs with PRRSV-1"]}]}],"canonical_facts":{"dc:contributor":["Rowland, Raymond R","Love, James F","Fang, Ying"],"dc:creator":["Salgado, Brianna Crystine"],"dc:date":["2024-05","2024-04-29"],"dc:description":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-09-16 without embargo terms","The student, Brianna Salgado, accepted the attached license on 2024-04-22 at 13:42.","The student, Brianna Salgado, submitted this Thesis for approval on 2024-04-22 at 13:53.","This Thesis was approved for publication on 2024-04-29 at 12:54.","DSpace SAF Submission Ingestion Package generated from Vireo submission #20544 on 2024-09-16 at 00:35:47","Sialoadhesin (Sn/SIGLEC1/CD169) is a macrophage surface protein belonging to a family of sialic acid binding immunoglobulin-like lectins. Classic models of PRRSV infection show that the first step in the infection of a macrophage is the binding of CD169 with the virion surface protein GP5. Previously, our lab discovered that CD169 knockout (KO) pigs support productive infection with PRRSV-2. Since PRRSV-1 and PRRSV-2 share only about 70% nucleotide identity and possess different requirements for infection of macrophages, we conducted a second study to determine if CD169 KO pigs could be infected with a PRRSV-1 isolate. KO pigs (n=8) and wild type pigs (WT, n=9) were infected with EU PRRSV-1 strain, SD01-08, and maintained for 28 days. Both genotypes were maintained in the same room and personnel were blind as to the genotype of individual pigs. Serum and tissues were collected and evaluated for infection using a commercial RT-qPCR kit. Antibody was measured using a commercial ELISA kit from IDEXX. Lung, tonsil, and submandibular lymph node tissue were extracted on day 28 for evaluation of viral RNA. Results from Serum and ELISA assays showed that all pigs in the wild-type (WT) and KO pigs supported PRRSV infection. Significant differences were observed in viral nucleic acid between WT and KO pigs at 21 (p = 0.002) and 28 (p = 0.023) days after infection. ELISA antibody analysis revealed statistically significant differences at 28 days post infection (p = 0 .021). Area under the curve analysis of viremia showed that the CD169 KO pigs supported higher virus loads. Analysis of tissues revealed no statistical differences between KO and WT pigs in tonsil and lymph node, while virus nucleic acid template copies were significantly higher in lung tissue from KO pigs. These results confirm that the presence of CD169 is not biologically relevant for PRRSV infection. Increased levels of viremia in KO pigs may be related to immune modulation by CD169, given the receptor is responsible for cell-to-cell interactions during antigen processing and presentation."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/124364"],"dc:language":["en","eng"],"dc:rights":["Copyright 2024 Brianna Salgado"],"dc:subject":["Prrsv-1 Sialoadhesin Cd169 Sn"],"dc:title":["Sialoadhesin is not required for infection of pigs with PRRSV-1"],"dc:type":["text"],"thesis:degree_discipline":["VMS - Pathobiology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:00Z"}