{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/124151"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/124151","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Investigating the epidemiology of adenoviruses in free-ranging turtles in Illinois","abstract":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-09-16 without embargo terms","abstract_html":"Submission original under an indefinite embargo labeled &#x27;Open Access&#x27;. The submission was exported from vireo on 2024-09-16 without embargo terms","abstract_has_math":false,"creators":["Ready, Zachary C."],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"VMS-Veterinary Clinical Medcne","degree_department":null,"school":null,"contributors":["Allender, Matthew C","Adamovicz, Laura","Langan, Jennifer","Dreslik, Michael"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2024,"date_issued":"2024-05","date_published":"2024-05","updated_at":"2026-07-22T22:25:00Z","subjects":["Adenovirus","Blanding's Turtle","Chrysemys Picta","Emydoidea Blandingii","Painted Turtle","Red-eared Slider","Sulawesi Tortoise Adenovirus","Trachemys Scripta Elegans"],"languages":["en","eng"],"rights":["Copyright 2024 Zachary Ready"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/124151","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Allender, Matthew C","Adamovicz, Laura","Langan, Jennifer","Dreslik, Michael"]},{"key":"dc:creator","label":"Author","values":["Ready, Zachary C."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2024-05","2024-03-25"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["VMS-Veterinary Clinical Medcne"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Adenovirus","Blanding's Turtle","Chrysemys Picta","Emydoidea Blandingii","Painted Turtle","Red-eared Slider","Sulawesi Tortoise Adenovirus","Trachemys Scripta Elegans"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2024 Zachary Ready"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/124151"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-09-16 without embargo terms","The student, Zachary Ready, accepted the attached license on 2024-03-06 at 16:55.","The student, Zachary Ready, submitted this Thesis for approval on 2024-03-06 at 17:03.","This Thesis was approved for publication on 2024-03-25 at 15:16.","DSpace SAF Submission Ingestion Package generated from Vireo submission #20246 on 2024-09-16 at 00:33:27","Adenoviruses (AdV) were first reported as a disease threat in chelonians during a mortality event in 2007 caused by a Siadenovirus later named Sulawesi tortoise adenovirus (STADV). Adenoviruses of another lineage, Testadenovirus, have been detected in multiple managed and free-ranging turtles in North America and Europe with variable or unknown relationships to clinical disease. This leaves a large gap in understanding the prevalence and impact of AdV in many turtle species. Therefore, a multi-species investigation to detect novel or existing adenoviruses in Blanding’s turtles (Emydoidea blandingii), painted turtles (Chrysemys picta) and red-eared sliders (Trachemys scripta elegans) using conventional PCR across four counties in Illinois from 2016-2022 was performed. Ten AdV were identified across the three species, with STADV being the most common virus species detected. Subsequently, a highly sensitive and specific TaqMan quantitative PCR assay targeting the DNA polymerase gene of STADV was developed. The newly developed and validated qPCR assay was used to test oral cloacal samples from Blanding’s turtles, painted turtles and red-eared sliders sampled across three Illinois counties from 2017-2022. The prevalence of STADV was 2.4% for Blanding’s turtle samples (n=14), 14.9% for painted turtle samples (n=24), and 45.1% for red-eared slider samples (n=37). Clinical signs associated with STADV detection included quiet, alert, responsive (QAR) mentation (p=0.002), pink mucous membranes (p<0.001), carapacial abnormalities (p=0.036), and plastron abnormalities (p=0.003). These results provide a baseline for the presence and diversity of AdV in free-ranging turtles in Illinois, including evidence that freshwater emydid turtles, such as red-eared sliders or painted turtles, may be the North American reservoir for STADV."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Investigating the epidemiology of adenoviruses in free-ranging turtles in Illinois"]}]}],"canonical_facts":{"dc:contributor":["Allender, Matthew C","Adamovicz, Laura","Langan, Jennifer","Dreslik, Michael"],"dc:creator":["Ready, Zachary C."],"dc:date":["2024-05","2024-03-25"],"dc:description":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-09-16 without embargo terms","The student, Zachary Ready, accepted the attached license on 2024-03-06 at 16:55.","The student, Zachary Ready, submitted this Thesis for approval on 2024-03-06 at 17:03.","This Thesis was approved for publication on 2024-03-25 at 15:16.","DSpace SAF Submission Ingestion Package generated from Vireo submission #20246 on 2024-09-16 at 00:33:27","Adenoviruses (AdV) were first reported as a disease threat in chelonians during a mortality event in 2007 caused by a Siadenovirus later named Sulawesi tortoise adenovirus (STADV). Adenoviruses of another lineage, Testadenovirus, have been detected in multiple managed and free-ranging turtles in North America and Europe with variable or unknown relationships to clinical disease. This leaves a large gap in understanding the prevalence and impact of AdV in many turtle species. Therefore, a multi-species investigation to detect novel or existing adenoviruses in Blanding’s turtles (Emydoidea blandingii), painted turtles (Chrysemys picta) and red-eared sliders (Trachemys scripta elegans) using conventional PCR across four counties in Illinois from 2016-2022 was performed. Ten AdV were identified across the three species, with STADV being the most common virus species detected. Subsequently, a highly sensitive and specific TaqMan quantitative PCR assay targeting the DNA polymerase gene of STADV was developed. The newly developed and validated qPCR assay was used to test oral cloacal samples from Blanding’s turtles, painted turtles and red-eared sliders sampled across three Illinois counties from 2017-2022. The prevalence of STADV was 2.4% for Blanding’s turtle samples (n=14), 14.9% for painted turtle samples (n=24), and 45.1% for red-eared slider samples (n=37). Clinical signs associated with STADV detection included quiet, alert, responsive (QAR) mentation (p=0.002), pink mucous membranes (p<0.001), carapacial abnormalities (p=0.036), and plastron abnormalities (p=0.003). These results provide a baseline for the presence and diversity of AdV in free-ranging turtles in Illinois, including evidence that freshwater emydid turtles, such as red-eared sliders or painted turtles, may be the North American reservoir for STADV."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/124151"],"dc:language":["en","eng"],"dc:rights":["Copyright 2024 Zachary Ready"],"dc:subject":["Adenovirus","Blanding's Turtle","Chrysemys Picta","Emydoidea Blandingii","Painted Turtle","Red-eared Slider","Sulawesi Tortoise Adenovirus","Trachemys Scripta Elegans"],"dc:title":["Investigating the epidemiology of adenoviruses in free-ranging turtles in Illinois"],"dc:type":["text"],"thesis:degree_discipline":["VMS-Veterinary Clinical Medcne"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:00Z"}