{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/122241"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/122241","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Droplet microfluidics for the study of multiphase fluid dynamics and the development of synthetic cells","abstract":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-12-01","abstract_html":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-12-01","abstract_has_math":false,"creators":["Jing, Wenyang"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biophysics & Quant Biology","degree_department":null,"school":null,"contributors":["Han, Hee-Sun","Ewoldt, Randy H.","Lu, Ting","Wu, Nicholas C.H."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2023,"date_issued":"2023-12","date_published":"2023-12","updated_at":"2026-07-22T22:25:00Z","subjects":["Microfluidics","Drop","Guv","Synthetic Cell","Droplet"],"languages":["en","eng"],"rights":["Copyright 2023 Wenyang Jing"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/122241","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Han, Hee-Sun","Ewoldt, Randy H.","Lu, Ting","Wu, Nicholas C.H."]},{"key":"dc:creator","label":"Author","values":["Jing, Wenyang"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2023-12","2023-12-01"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biophysics & Quant Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Microfluidics","Drop","Guv","Synthetic Cell","Droplet"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2023 Wenyang Jing"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/122241"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-12-01","The student, Wenyang Jing, accepted the attached license on 2023-11-27 at 19:36.","The student, Wenyang Jing, submitted this Dissertation for approval on 2023-11-27 at 19:44.","This Dissertation was approved for publication on 2023-12-01 at 08:42.","DSpace SAF Submission Ingestion Package generated from Vireo submission #20031 on 2024-03-01 at 13:50:30","Microfluidics has seen wide use for both enabling new technologies and for fundamental studies owing to its virtues of small volumes, fluid physics and manipulation at the microscale, and integrated functionalities of actuation, sensing, and separation. Droplet microfluidics possesses these general advantages and adds on top of that multiphase flow dynamics and analyte compartmentalization, making it particularly useful for bioassays such as single-cell RNA sequencing and digital polymerase chain reaction (ddPCR), for example. This thesis describes work that utilizes droplet microfluidics both as a as a tool for biological applications and as a system for investigating multiphase fluid dynamics. For the former, droplet microfluidics is utilized for single-molecule detection of circular RNAs (circRNAs) by ddPCR and for templating giant unilamellar vesicles (GUVs) toward the bottom-up assembly of synthetic cells using both single and double emulsions. Fundamental fluid dynamics are also studied. Inertial ordering of multi-droplet trains was investigated, where hydrodynamic interactions are shown to be enable the self-assembly of droplets into well-spaced trains or lead to disorder in contrast to the established observations involving solid particles. These dynamics also have utility toward droplet manipulation for bioassays. Finally, fluid dynamics were investigated for both separating GUVs by size and for their production using the dewetting of double emulsions."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Droplet microfluidics for the study of multiphase fluid dynamics and the development of synthetic cells"]}]}],"canonical_facts":{"dc:contributor":["Han, Hee-Sun","Ewoldt, Randy H.","Lu, Ting","Wu, Nicholas C.H."],"dc:creator":["Jing, Wenyang"],"dc:date":["2023-12","2023-12-01"],"dc:description":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-12-01","The student, Wenyang Jing, accepted the attached license on 2023-11-27 at 19:36.","The student, Wenyang Jing, submitted this Dissertation for approval on 2023-11-27 at 19:44.","This Dissertation was approved for publication on 2023-12-01 at 08:42.","DSpace SAF Submission Ingestion Package generated from Vireo submission #20031 on 2024-03-01 at 13:50:30","Microfluidics has seen wide use for both enabling new technologies and for fundamental studies owing to its virtues of small volumes, fluid physics and manipulation at the microscale, and integrated functionalities of actuation, sensing, and separation. Droplet microfluidics possesses these general advantages and adds on top of that multiphase flow dynamics and analyte compartmentalization, making it particularly useful for bioassays such as single-cell RNA sequencing and digital polymerase chain reaction (ddPCR), for example. This thesis describes work that utilizes droplet microfluidics both as a as a tool for biological applications and as a system for investigating multiphase fluid dynamics. For the former, droplet microfluidics is utilized for single-molecule detection of circular RNAs (circRNAs) by ddPCR and for templating giant unilamellar vesicles (GUVs) toward the bottom-up assembly of synthetic cells using both single and double emulsions. Fundamental fluid dynamics are also studied. Inertial ordering of multi-droplet trains was investigated, where hydrodynamic interactions are shown to be enable the self-assembly of droplets into well-spaced trains or lead to disorder in contrast to the established observations involving solid particles. These dynamics also have utility toward droplet manipulation for bioassays. Finally, fluid dynamics were investigated for both separating GUVs by size and for their production using the dewetting of double emulsions."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/122241"],"dc:language":["en","eng"],"dc:rights":["Copyright 2023 Wenyang Jing"],"dc:subject":["Microfluidics","Drop","Guv","Synthetic Cell","Droplet"],"dc:title":["Droplet microfluidics for the study of multiphase fluid dynamics and the development of synthetic cells"],"dc:type":["text"],"thesis:degree_discipline":["Biophysics & Quant Biology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:00Z"}