{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/122129"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/122129","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Development of a broadly protective protein-based multivalent vaccine against cholera and beyond","abstract":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-12-01","abstract_html":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-12-01","abstract_has_math":false,"creators":["Upadhyay, Ipshita"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"VMS - Pathobiology","degree_department":null,"school":null,"contributors":["Zhang, Weiping","Maddox, Carol","Lau, Gee","Steelman, Andrew"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2023,"date_issued":"2023-12","date_published":"2023-12","updated_at":"2026-07-22T22:25:00Z","subjects":["Vaccine","Cholera","Pre-clinical","Mice","Rabbits","Mefa","In-silico","Computational","Cholera Mefa","Vibrio Cholerae"],"languages":["en","eng"],"rights":["Copyright 2023 Ipshita Upadhyay"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/122129","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Zhang, Weiping","Maddox, Carol","Lau, Gee","Steelman, Andrew"]},{"key":"dc:creator","label":"Author","values":["Upadhyay, Ipshita"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2023-12","2023-11-30"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["VMS - Pathobiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Vaccine","Cholera","Pre-clinical","Mice","Rabbits","Mefa","In-silico","Computational","Cholera Mefa","Vibrio Cholerae"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2023 Ipshita Upadhyay"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/122129"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-12-01","The student, Ipshita Upadhyay, accepted the attached license on 2023-11-20 at 15:52.","The student, Ipshita Upadhyay, submitted this Dissertation for approval on 2023-11-20 at 16:13.","This Dissertation was approved for publication on 2023-11-30 at 13:12.","DSpace SAF Submission Ingestion Package generated from Vireo submission #19979 on 2024-03-01 at 13:30:41","Vibrio cholerae continues to be a significant global health concern. Using multiepitope fusion antigen (MEFA) vaccinology platform we developed cholera MEFA which presents antigenic domains from virulence factors of V. cholerae, including toxin coregulated pilus A, cholera toxin (CT), sialidase, hemolysin A, and flagellins B, C, and D along with peptide mimics of lipopolysaccharide (LPS). Cholera MEFA elicited antibodies targeting all the virulence factors except LPS in mice. Additionally, adult rabbits demonstrated a remarkable 2-log (99%) reduction in V. cholerae colonization, and newborn rabbits born to mothers who received cholera MEFA acquired antigen-specific antibodies passively, resulting in protection from bacterial intestinal colonization and diarrhea following the challenge with four different strains of V. cholerae. Moreover, in mice, cholera MEFA elicited high immunogenic titers at a low dose of 5µg. It was further observed that on co-administering cholera MEFA with ETEC MEFA, both vaccine candidates retained their immunogenicity in mice against included virulent antigens. Overall, this study underscores the broad immunogenic and cross-protective nature of the polyvalent cholera protein, suggesting its potential as an antigen for a cross-protective cholera vaccine. The use of the infant rabbit passive protection model represents a crucial advancement in preclinical efficacy assessment and may help overcome hurdles in the development of cholera vaccine. The comparable immunogenicity on co-administration with ETEC MEFA further validates the potential use of cholera MEFA in resource-limited settings."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Development of a broadly protective protein-based multivalent vaccine against cholera and beyond"]}]}],"canonical_facts":{"dc:contributor":["Zhang, Weiping","Maddox, Carol","Lau, Gee","Steelman, Andrew"],"dc:creator":["Upadhyay, Ipshita"],"dc:date":["2023-12","2023-11-30"],"dc:description":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-12-01","The student, Ipshita Upadhyay, accepted the attached license on 2023-11-20 at 15:52.","The student, Ipshita Upadhyay, submitted this Dissertation for approval on 2023-11-20 at 16:13.","This Dissertation was approved for publication on 2023-11-30 at 13:12.","DSpace SAF Submission Ingestion Package generated from Vireo submission #19979 on 2024-03-01 at 13:30:41","Vibrio cholerae continues to be a significant global health concern. Using multiepitope fusion antigen (MEFA) vaccinology platform we developed cholera MEFA which presents antigenic domains from virulence factors of V. cholerae, including toxin coregulated pilus A, cholera toxin (CT), sialidase, hemolysin A, and flagellins B, C, and D along with peptide mimics of lipopolysaccharide (LPS). Cholera MEFA elicited antibodies targeting all the virulence factors except LPS in mice. Additionally, adult rabbits demonstrated a remarkable 2-log (99%) reduction in V. cholerae colonization, and newborn rabbits born to mothers who received cholera MEFA acquired antigen-specific antibodies passively, resulting in protection from bacterial intestinal colonization and diarrhea following the challenge with four different strains of V. cholerae. Moreover, in mice, cholera MEFA elicited high immunogenic titers at a low dose of 5µg. It was further observed that on co-administering cholera MEFA with ETEC MEFA, both vaccine candidates retained their immunogenicity in mice against included virulent antigens. Overall, this study underscores the broad immunogenic and cross-protective nature of the polyvalent cholera protein, suggesting its potential as an antigen for a cross-protective cholera vaccine. The use of the infant rabbit passive protection model represents a crucial advancement in preclinical efficacy assessment and may help overcome hurdles in the development of cholera vaccine. The comparable immunogenicity on co-administration with ETEC MEFA further validates the potential use of cholera MEFA in resource-limited settings."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/122129"],"dc:language":["en","eng"],"dc:rights":["Copyright 2023 Ipshita Upadhyay"],"dc:subject":["Vaccine","Cholera","Pre-clinical","Mice","Rabbits","Mefa","In-silico","Computational","Cholera Mefa","Vibrio Cholerae"],"dc:title":["Development of a broadly protective protein-based multivalent vaccine against cholera and beyond"],"dc:type":["text"],"thesis:degree_discipline":["VMS - Pathobiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:00Z"}