{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/122072"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/122072","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Factors influencing vitamin a secretion in macrophages","abstract":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-03-01 without embargo terms","abstract_html":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-03-01 without embargo terms","abstract_has_math":false,"creators":["Sim, Jaeyoung"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Food Science & Human Nutrition","degree_department":null,"school":null,"contributors":["Amengual, Jaume"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2023,"date_issued":"2023-12","date_published":"2023-12","updated_at":"2026-07-22T22:25:00Z","subjects":["Vitamin A","Macrophages"],"languages":["en","eng"],"rights":["Copyright 2023 Jaeyoung Sim"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/122072","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Amengual, Jaume"]},{"key":"dc:creator","label":"Author","values":["Sim, Jaeyoung"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2023-12","2023-12-08"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Food Science & Human Nutrition"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Vitamin A","Macrophages"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2023 Jaeyoung Sim"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/122072"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-03-01 without embargo terms","The student, Jaeyoung Sim, accepted the attached license on 2023-12-06 at 18:03.","The student, Jaeyoung Sim, submitted this Thesis for approval on 2023-12-06 at 18:09.","This Thesis was approved for publication on 2023-12-08 at 09:10.","DSpace SAF Submission Ingestion Package generated from Vireo submission #20161 on 2024-03-01 at 13:15:30","Vitamin A plays a crucial role in modulating macrophage function. Our group recently showed that retinoic acid, the transcriptionally active form of vitamin A, is released by cultured macrophages, which could influence the differentiation of other cell types such as T-cells. The objective of this study is to elucidate the mechanisms that regulate vitamin A secretion from macrophages. Using a method developed in our lab that allows us to load cells with vitamin A, we compared the release of retinoids (retinol and retinyl esters) between macrophages and hepatocytes by performing kinetic studies. Next, we focused on the mechanisms that regulate vitamin A secretion in the macrophage. We measured intracellular (cell lysates) and extracellular (media) retinoids by high-performance liquid chromatography. To examine the mechanisms implicated in vitamin A release, cells were exposed to different experimental conditions such as chloroquine to block lysosomal degradation and GW4869 to block exosome formation. We also utilized the macrophages isolated from apolipoprotein E and retinol-binding protein 4 deficient mice to test the role of these transporters on vitamin A release in macrophages. Blocking lysosomal activity led to an increase in intracellular retinyl ester levels without affecting retinoid secretion. The inhibition of exosome formation reduced retinoid secretion from macrophages, and vitamin A was present in purified exosomes, suggesting that the exosomal pathway contributes to vitamin A secretion from the macrophage."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Factors influencing vitamin a secretion in macrophages"]}]}],"canonical_facts":{"dc:contributor":["Amengual, Jaume"],"dc:creator":["Sim, Jaeyoung"],"dc:date":["2023-12","2023-12-08"],"dc:description":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-03-01 without embargo terms","The student, Jaeyoung Sim, accepted the attached license on 2023-12-06 at 18:03.","The student, Jaeyoung Sim, submitted this Thesis for approval on 2023-12-06 at 18:09.","This Thesis was approved for publication on 2023-12-08 at 09:10.","DSpace SAF Submission Ingestion Package generated from Vireo submission #20161 on 2024-03-01 at 13:15:30","Vitamin A plays a crucial role in modulating macrophage function. Our group recently showed that retinoic acid, the transcriptionally active form of vitamin A, is released by cultured macrophages, which could influence the differentiation of other cell types such as T-cells. The objective of this study is to elucidate the mechanisms that regulate vitamin A secretion from macrophages. Using a method developed in our lab that allows us to load cells with vitamin A, we compared the release of retinoids (retinol and retinyl esters) between macrophages and hepatocytes by performing kinetic studies. Next, we focused on the mechanisms that regulate vitamin A secretion in the macrophage. We measured intracellular (cell lysates) and extracellular (media) retinoids by high-performance liquid chromatography. To examine the mechanisms implicated in vitamin A release, cells were exposed to different experimental conditions such as chloroquine to block lysosomal degradation and GW4869 to block exosome formation. We also utilized the macrophages isolated from apolipoprotein E and retinol-binding protein 4 deficient mice to test the role of these transporters on vitamin A release in macrophages. Blocking lysosomal activity led to an increase in intracellular retinyl ester levels without affecting retinoid secretion. The inhibition of exosome formation reduced retinoid secretion from macrophages, and vitamin A was present in purified exosomes, suggesting that the exosomal pathway contributes to vitamin A secretion from the macrophage."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/122072"],"dc:language":["en","eng"],"dc:rights":["Copyright 2023 Jaeyoung Sim"],"dc:subject":["Vitamin A","Macrophages"],"dc:title":["Factors influencing vitamin a secretion in macrophages"],"dc:type":["text"],"thesis:degree_discipline":["Food Science & Human Nutrition"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:00Z"}