{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/122059"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/122059","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Structural evolution of lipid droplets in murine liver cancer","abstract":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-03-01 without embargo terms","abstract_html":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-03-01 without embargo terms","abstract_has_math":false,"creators":["Li, Lily Xueqi"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Materials Science & Engr","degree_department":null,"school":null,"contributors":["Leal, Cecilia"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2023,"date_issued":"2023-12","date_published":"2023-12","updated_at":"2026-07-22T22:25:00Z","subjects":["Lipid Droplets","Cancer","Triacylglycerols","Cholesterol Esters"],"languages":["en","eng"],"rights":["Copyright 2023 Lily Li"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/122059","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Leal, Cecilia"]},{"key":"dc:creator","label":"Author","values":["Li, Lily Xueqi"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2023-12","2023-12-07"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Materials Science & Engr"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Lipid Droplets","Cancer","Triacylglycerols","Cholesterol Esters"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2023 Lily Li"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/122059"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-03-01 without embargo terms","The student, Lily Li, accepted the attached license on 2023-12-04 at 17:17.","The student, Lily Li, submitted this Thesis for approval on 2023-12-04 at 17:24.","This Thesis was approved for publication on 2023-12-07 at 16:46.","DSpace SAF Submission Ingestion Package generated from Vireo submission #20134 on 2024-03-01 at 13:15:25","Hepatocellular carcinoma (HCC) is a primary liver cancer that arises from hepatocytes, the main functional cells of the liver. It represents a major global health concern due to its high incidence and often late-stage diagnosis, making it one of the leading causes of cancer-related deaths worldwide. A notable observation in the context of HCC is its shared characteristics with fatty liver disease which is characterized by the accumulation of excess fat in liver cells. As lipid droplets (LDs) are cytosolic fat storage organelles, they have gained increasing recognition for their significant involvement in liver diseases from recent research. However, how LDs manage and pack a surplus of fat during tumorigenesis remains unknown. We conducted a comprehensive study utilizing micropipette aspiration, X-ray scattering, cryo-EM, and lipidomics to elucidate how the morphology, composition, structure, and mechanical properties of LDs evolve during tumorigenesis. Our study discovers that LDs extracted from mice with liver cancer display an unbalanced ratio of cholesterol esters (CEs) to triacylglycerols (TAGs). This imbalance induces an unexpected structural transformation where CEs and TAGs close pack into a multilamellar structure instead of being present in a liquid-like state. This structural evolution is accompanied by a remarkable increase in stiffness seen by a doubling of the area expansivity modulus of LDs compared to those from normal liver tissue. These findings provide valuable insights into the role of LDs’ biophysical properties during liver cancer progression."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Structural evolution of lipid droplets in murine liver cancer"]}]}],"canonical_facts":{"dc:contributor":["Leal, Cecilia"],"dc:creator":["Li, Lily Xueqi"],"dc:date":["2023-12","2023-12-07"],"dc:description":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-03-01 without embargo terms","The student, Lily Li, accepted the attached license on 2023-12-04 at 17:17.","The student, Lily Li, submitted this Thesis for approval on 2023-12-04 at 17:24.","This Thesis was approved for publication on 2023-12-07 at 16:46.","DSpace SAF Submission Ingestion Package generated from Vireo submission #20134 on 2024-03-01 at 13:15:25","Hepatocellular carcinoma (HCC) is a primary liver cancer that arises from hepatocytes, the main functional cells of the liver. It represents a major global health concern due to its high incidence and often late-stage diagnosis, making it one of the leading causes of cancer-related deaths worldwide. A notable observation in the context of HCC is its shared characteristics with fatty liver disease which is characterized by the accumulation of excess fat in liver cells. As lipid droplets (LDs) are cytosolic fat storage organelles, they have gained increasing recognition for their significant involvement in liver diseases from recent research. However, how LDs manage and pack a surplus of fat during tumorigenesis remains unknown. We conducted a comprehensive study utilizing micropipette aspiration, X-ray scattering, cryo-EM, and lipidomics to elucidate how the morphology, composition, structure, and mechanical properties of LDs evolve during tumorigenesis. Our study discovers that LDs extracted from mice with liver cancer display an unbalanced ratio of cholesterol esters (CEs) to triacylglycerols (TAGs). This imbalance induces an unexpected structural transformation where CEs and TAGs close pack into a multilamellar structure instead of being present in a liquid-like state. This structural evolution is accompanied by a remarkable increase in stiffness seen by a doubling of the area expansivity modulus of LDs compared to those from normal liver tissue. These findings provide valuable insights into the role of LDs’ biophysical properties during liver cancer progression."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/122059"],"dc:language":["en","eng"],"dc:rights":["Copyright 2023 Lily Li"],"dc:subject":["Lipid Droplets","Cancer","Triacylglycerols","Cholesterol Esters"],"dc:title":["Structural evolution of lipid droplets in murine liver cancer"],"dc:type":["text"],"thesis:degree_discipline":["Materials Science & Engr"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:00Z"}