{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/121965"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/121965","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Effects of lateralized hippocampal damage and pituitary gonadotrope dysfunction in a mouse model of temporal lobe epilepsy","abstract":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-03-01 without embargo terms","abstract_html":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-03-01 without embargo terms","abstract_has_math":false,"creators":["Cutia, Cathryn A"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Neuroscience","degree_department":null,"school":null,"contributors":["Christian-Hinman, Catherine","Llano, Daniel","Raetzman, Lori","Gritton, Howard"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2023,"date_issued":"2023-12","date_published":"2023-12","updated_at":"2026-07-22T22:25:00Z","subjects":["Reproductive Endocrine Dysfunction","Mouse Model","Temporal Lobe Epilepsy"],"languages":["en","eng"],"rights":["Copyright 2023 Cathryn Cutia"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/121965","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Christian-Hinman, Catherine","Llano, Daniel","Raetzman, Lori","Gritton, Howard"]},{"key":"dc:creator","label":"Author","values":["Cutia, Cathryn A"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2023-12","2023-11-01"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Neuroscience"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Reproductive Endocrine Dysfunction","Mouse Model","Temporal Lobe Epilepsy"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2023 Cathryn Cutia"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/121965"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-03-01 without embargo terms","The student, Cathryn Cutia, accepted the attached license on 2023-10-27 at 11:28.","The student, Cathryn Cutia, submitted this Dissertation for approval on 2023-10-27 at 11:36.","This Dissertation was approved for publication on 2023-11-01 at 15:47.","DSpace SAF Submission Ingestion Package generated from Vireo submission #19877 on 2024-03-01 at 13:14:01","Reproductive endocrine dysfunction is a common comorbidity of temporal lobe epilepsy (TLE). The specific mechanisms that link temporal lobe seizures to reproductive endocrine dysfunction remain unknown. However, clinical reports suggest that epilepsy-associated hypothalamic-pituitary-gonadal (HPG) axis dysfunction likely underlies the development of reproductive endocrine disorders. The intrahippocampal kainic acid (IHKA) mouse model of TLE also shows reproductive endocrine dysfunction as a majority of female IHKA treated animals display estrous cycle disruption. Furthermore, IHKA mice display alterations in the HPG axis as the activity of, and input to, hypothalamic gonadotropin-releasing hormone (GnRH) neurons is changed in IHKA females that develop estrous cycle elongation. However, further work in this model is needed to identify a specific mechanism that links the epilepsy-associated changes in hypothalamic circuitry to the development of reproductive endocrine dysfunction. The overall goal of this dissertation is to evaluate the progression and robustness of the reproductive endocrine dysfunction phenotype in female IHKA mice and to determine if epilepsy-associated hypothalamic changes in this model impact the pituitary. Chapter 2 investigates the differences in the presentation and progression of pituitary and gonadal phenotypic outcomes in female mice injected in the left vs. right dorsal hippocampus with KA. Chapter 3 continues to investigate the impacts of IHKA injection site by characterizing seizure burden differences in the left vs. right IHKA mice. Chapter 4 investigates the epilepsy-associated changes in pituitary release of gonadotropins and sensitivity to GnRH. The results from Chapter 2 and 3 indicate that the side of IHKA injection does not impact the development of comorbid estrous cycle disruption in females, but that KA injection site can impact pituitary gene expression and seizure burden in a sex- and estrous cycle-dependent manner. The results from Chapter 4 indicate that there are epilepsy-associated elevations in pituitary sensitivity to GnRH on certain estrous cycle stages in the absence of changes to the pattern of gonadotropin release. Overall, the data in this dissertation demonstrate that, within the IHKA model, lateralization of hippocampal damage can have implications on phenotypes present, and that temporal lobe seizures lead to altered pituitary function. This data demonstrates the usefulness of the IHKA model in the study of mechanisms linking temporal lobe seizures to altered reproductive endocrine function and vice versa."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Effects of lateralized hippocampal damage and pituitary gonadotrope dysfunction in a mouse model of temporal lobe epilepsy"]}]}],"canonical_facts":{"dc:contributor":["Christian-Hinman, Catherine","Llano, Daniel","Raetzman, Lori","Gritton, Howard"],"dc:creator":["Cutia, Cathryn A"],"dc:date":["2023-12","2023-11-01"],"dc:description":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-03-01 without embargo terms","The student, Cathryn Cutia, accepted the attached license on 2023-10-27 at 11:28.","The student, Cathryn Cutia, submitted this Dissertation for approval on 2023-10-27 at 11:36.","This Dissertation was approved for publication on 2023-11-01 at 15:47.","DSpace SAF Submission Ingestion Package generated from Vireo submission #19877 on 2024-03-01 at 13:14:01","Reproductive endocrine dysfunction is a common comorbidity of temporal lobe epilepsy (TLE). The specific mechanisms that link temporal lobe seizures to reproductive endocrine dysfunction remain unknown. However, clinical reports suggest that epilepsy-associated hypothalamic-pituitary-gonadal (HPG) axis dysfunction likely underlies the development of reproductive endocrine disorders. The intrahippocampal kainic acid (IHKA) mouse model of TLE also shows reproductive endocrine dysfunction as a majority of female IHKA treated animals display estrous cycle disruption. Furthermore, IHKA mice display alterations in the HPG axis as the activity of, and input to, hypothalamic gonadotropin-releasing hormone (GnRH) neurons is changed in IHKA females that develop estrous cycle elongation. However, further work in this model is needed to identify a specific mechanism that links the epilepsy-associated changes in hypothalamic circuitry to the development of reproductive endocrine dysfunction. The overall goal of this dissertation is to evaluate the progression and robustness of the reproductive endocrine dysfunction phenotype in female IHKA mice and to determine if epilepsy-associated hypothalamic changes in this model impact the pituitary. Chapter 2 investigates the differences in the presentation and progression of pituitary and gonadal phenotypic outcomes in female mice injected in the left vs. right dorsal hippocampus with KA. Chapter 3 continues to investigate the impacts of IHKA injection site by characterizing seizure burden differences in the left vs. right IHKA mice. Chapter 4 investigates the epilepsy-associated changes in pituitary release of gonadotropins and sensitivity to GnRH. The results from Chapter 2 and 3 indicate that the side of IHKA injection does not impact the development of comorbid estrous cycle disruption in females, but that KA injection site can impact pituitary gene expression and seizure burden in a sex- and estrous cycle-dependent manner. The results from Chapter 4 indicate that there are epilepsy-associated elevations in pituitary sensitivity to GnRH on certain estrous cycle stages in the absence of changes to the pattern of gonadotropin release. Overall, the data in this dissertation demonstrate that, within the IHKA model, lateralization of hippocampal damage can have implications on phenotypes present, and that temporal lobe seizures lead to altered pituitary function. This data demonstrates the usefulness of the IHKA model in the study of mechanisms linking temporal lobe seizures to altered reproductive endocrine function and vice versa."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/121965"],"dc:language":["en","eng"],"dc:rights":["Copyright 2023 Cathryn Cutia"],"dc:subject":["Reproductive Endocrine Dysfunction","Mouse Model","Temporal Lobe Epilepsy"],"dc:title":["Effects of lateralized hippocampal damage and pituitary gonadotrope dysfunction in a mouse model of temporal lobe epilepsy"],"dc:type":["text"],"thesis:degree_discipline":["Neuroscience"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:00Z"}