{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/121500"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/121500","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Spatial light interference microscopy (slim) measurements of hypothermia in an experimental cellular model of traumatic brain injury","abstract":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2023-12-04 without embargo terms","abstract_html":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2023-12-04 without embargo terms","abstract_has_math":false,"creators":["Lee, Young Jae"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Neuroscience","degree_department":null,"school":null,"contributors":["Best-Popescu, Catherine","Chung, Hee Jung","Yoo, Dongwan","Allen, Jont"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2023,"date_issued":"2023-08","date_published":"2023-08","updated_at":"2026-07-22T22:24:57Z","subjects":["Traumatic Brain Injury","Tbi","Spatial Light Interference Microscopy","Slim","Fluid Percussion Injury","Fpi","Hypothermia","Therapeutic Hypothermia","Th","Quantitative Phase Imaging","Qpi","Dispersion-relation Phase Spectroscopy","Dps","Dry Mass"],"languages":["en","eng"],"rights":["Copyright 2023 Young Jae Lee"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/121500","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Best-Popescu, Catherine","Chung, Hee Jung","Yoo, Dongwan","Allen, Jont"]},{"key":"dc:creator","label":"Author","values":["Lee, Young Jae"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2023-08","2023-07-14"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Neuroscience"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Traumatic Brain Injury","Tbi","Spatial Light Interference Microscopy","Slim","Fluid Percussion Injury","Fpi","Hypothermia","Therapeutic Hypothermia","Th","Quantitative Phase Imaging","Qpi","Dispersion-relation Phase Spectroscopy","Dps","Dry Mass"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2023 Young Jae Lee"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/121500"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2023-12-04 without embargo terms","The student, Young Jae Lee, accepted the attached license on 2023-07-11 at 12:56.","The student, Young Jae Lee, submitted this Dissertation for approval on 2023-07-11 at 13:06.","This Dissertation was approved for publication on 2023-07-14 at 10:26.","DSpace SAF Submission Ingestion Package generated from Vireo submission #19623 on 2023-12-04 at 17:01:50","Current neuroscience research conceptualizes the brain as a dynamic, complex cellular network whose connections are constantly changing as we progress through life. Traumatic Brain Injury (TBI) is a disruption in normal brain function following a sudden, external, physical force to the brain parenchyma. TBI is a leading cause of mortality and morbidity among civilians and military personnel worldwide. According to the Centers for Disease Control (CDC) there were over 69,000 TBI-related deaths in the United States in 2021. This equals approximately 190-TBI-related deaths every day. Therefore, it is crucial to understand the ways in which healthy and damaged central nervous system (CNS) cells flourish, that is grow, arborize, link, communicate, and repair. Despite several decades of research, no curative therapies for TBI-associated neurological, behavioral, and cognitive deficits exist. Both the underlying pathobiology and the identification of exploitable neural repair mechanisms need to be more fully investigated; specifically, at the secondary brain injury, since this stage is thought to be responsible for the appearance, progression, and long-term persistence of cognitive, neuropsychiatric, behavioral, and neurological deficits following TBI. Therefore, effective TBI models of disease and standardized quantitative assessment measures for TBI are imperative for testing TBI therapeutics and to facilitate successful TBI drug discovery in this stage. My Ph.D. training has been centered around quantifying neuronal biophysical changes in a Fluid Percussion Instrument (FPI) cellular model of brain injury. This dissertation reviews my research endeavors here at the University of Illinois at Urbana Champaign. First, we examine healthy neurons, and neural network dynamics with a novel Quantitative Phase Imaging (QPI) technique called Spatial Light Interference Microscopy (SLIM). In my first study, I observed that neurons display a significantly higher rate of neurite length growth under lower confluence conditions especially with faster axonal growth and more dynamic changes of dendritic growth over time. In higher confluence conditions, neuronal network also depicted ‘contact inhibition’ which shows slower or no growth over time. SLIM is an ideal imaging technique to study FPI induced neuronal damage to represent TBI because it provides time-lapse imaging of living cells over weeks while preserving cell viability. SLIM allows for the measurement of FPI-induced changes in neuronal survival and iii health overtime, and for the investigation of Therapeutic Hypothermia (TH) efficacy following TBI. Through these biophysical measurements from SLIM, we examined the effects of TH on FPI-induced injury on murine cortical neurons. We found that a five-hour treatment period of 32°C TH, administered within 2 hours of injury was optimally beneficial to cortical neurons in vitro following mild and moderate FPI, from less than 40% to 80% of live cell survivability. We didn’t find any beneficial effects of 32°C TH when it is administered before FPI injury. These findings may act as a springboard for standardizing future in vitro TBI therapeutic investigational studies to support the development of successful TBI therapeutics and furthermore to in vivo and clinical TBI studies."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Spatial light interference microscopy (slim) measurements of hypothermia in an experimental cellular model of traumatic brain injury"]}]}],"canonical_facts":{"dc:contributor":["Best-Popescu, Catherine","Chung, Hee Jung","Yoo, Dongwan","Allen, Jont"],"dc:creator":["Lee, Young Jae"],"dc:date":["2023-08","2023-07-14"],"dc:description":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2023-12-04 without embargo terms","The student, Young Jae Lee, accepted the attached license on 2023-07-11 at 12:56.","The student, Young Jae Lee, submitted this Dissertation for approval on 2023-07-11 at 13:06.","This Dissertation was approved for publication on 2023-07-14 at 10:26.","DSpace SAF Submission Ingestion Package generated from Vireo submission #19623 on 2023-12-04 at 17:01:50","Current neuroscience research conceptualizes the brain as a dynamic, complex cellular network whose connections are constantly changing as we progress through life. Traumatic Brain Injury (TBI) is a disruption in normal brain function following a sudden, external, physical force to the brain parenchyma. TBI is a leading cause of mortality and morbidity among civilians and military personnel worldwide. According to the Centers for Disease Control (CDC) there were over 69,000 TBI-related deaths in the United States in 2021. This equals approximately 190-TBI-related deaths every day. Therefore, it is crucial to understand the ways in which healthy and damaged central nervous system (CNS) cells flourish, that is grow, arborize, link, communicate, and repair. Despite several decades of research, no curative therapies for TBI-associated neurological, behavioral, and cognitive deficits exist. Both the underlying pathobiology and the identification of exploitable neural repair mechanisms need to be more fully investigated; specifically, at the secondary brain injury, since this stage is thought to be responsible for the appearance, progression, and long-term persistence of cognitive, neuropsychiatric, behavioral, and neurological deficits following TBI. Therefore, effective TBI models of disease and standardized quantitative assessment measures for TBI are imperative for testing TBI therapeutics and to facilitate successful TBI drug discovery in this stage. My Ph.D. training has been centered around quantifying neuronal biophysical changes in a Fluid Percussion Instrument (FPI) cellular model of brain injury. This dissertation reviews my research endeavors here at the University of Illinois at Urbana Champaign. First, we examine healthy neurons, and neural network dynamics with a novel Quantitative Phase Imaging (QPI) technique called Spatial Light Interference Microscopy (SLIM). In my first study, I observed that neurons display a significantly higher rate of neurite length growth under lower confluence conditions especially with faster axonal growth and more dynamic changes of dendritic growth over time. In higher confluence conditions, neuronal network also depicted ‘contact inhibition’ which shows slower or no growth over time. SLIM is an ideal imaging technique to study FPI induced neuronal damage to represent TBI because it provides time-lapse imaging of living cells over weeks while preserving cell viability. SLIM allows for the measurement of FPI-induced changes in neuronal survival and iii health overtime, and for the investigation of Therapeutic Hypothermia (TH) efficacy following TBI. Through these biophysical measurements from SLIM, we examined the effects of TH on FPI-induced injury on murine cortical neurons. We found that a five-hour treatment period of 32°C TH, administered within 2 hours of injury was optimally beneficial to cortical neurons in vitro following mild and moderate FPI, from less than 40% to 80% of live cell survivability. We didn’t find any beneficial effects of 32°C TH when it is administered before FPI injury. These findings may act as a springboard for standardizing future in vitro TBI therapeutic investigational studies to support the development of successful TBI therapeutics and furthermore to in vivo and clinical TBI studies."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/121500"],"dc:language":["en","eng"],"dc:rights":["Copyright 2023 Young Jae Lee"],"dc:subject":["Traumatic Brain Injury","Tbi","Spatial Light Interference Microscopy","Slim","Fluid Percussion Injury","Fpi","Hypothermia","Therapeutic Hypothermia","Th","Quantitative Phase Imaging","Qpi","Dispersion-relation Phase Spectroscopy","Dps","Dry Mass"],"dc:title":["Spatial light interference microscopy (slim) measurements of hypothermia in an experimental cellular model of traumatic brain injury"],"dc:type":["text"],"thesis:degree_discipline":["Neuroscience"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:57Z"}